Facilitating the enzymatic conversion of lysineto cadaverine in engineered Escherichia coli with metabolic regulation by genes deletion

Enhanced production of l-sorbose by systematic engineering of dehydrogenases in Gluconobacter oxydans

“…However, these methods did not increase the conversion rate of D-sorbitol to l -sorbose and reduce by-products in the fermentation process. Thus, gene knockout was necessary to decrease or eliminate the formation of by-products and improve substrate conversion; for example, the cadaverine production increased threefold with the deletion of some genes [ 37 ]. The present study showed that deletion of dehydrogenases could indeed improve the conversion rate of D-sorbitol to l -sorbose and reduce the formation of by-products.…”

Cellular Engineering and Biocatalysis Strategies toward Sustainable Cadaverine Production: State of the Art and Perspectives

“…36 Besides, Huang et al created different types of recombinant E. coli strains by overexpressing cadA-cadB genes and inactivating speE, speG, ygjG, and puuA genes as shown in Figure 2. 57 Results indicated that both modified strains increased cadaverine productivity and displayed higher tolerance to substrates. Especially, when four genes mentioned above were deleted, the highest cadaverine production was obtained with yield of 17.06 g/L after 40 h fermentation.…”

“…Besides, Huang et al created different types of recombinant E. coli strains by overexpressing cadA-cadB genes and inactivating speE, speG, ygjG, and puuA genes as shown in Figure . Results indicated that both modified strains increased cadaverine productivity and displayed higher tolerance to substrates.…”

Green chemical and biological synthesis of cadaverine: recent development and challenges

“…The optimized feeding can produce 221 g L −1 cadaverine with a molar yield of 92% in 16 h. 48 The other engineered strain E. coli BL21Δ spe EΔ puu AΔ spe GΔ ygj G (pSITDuet- cadA-pelB-cadB ) can also increase the yield of cadaverine. 49 Even though the CadB can increase the transfer rate of the l -lysine and cadaverine, other intensification should be developed to further improve the catalytic productivity.…”