Enhanced production of l-sorbose by systematic engineering of dehydrogenases in Gluconobacter oxydans

Rare sugar l-sorbose exerts antitumor activity by impairing glucose metabolism

“…L-Sorbose is the intermediate of a fermentation process for manufacturing vitamin C 34 . Although it is rare in nature, L-sorbose can be efficiently produced by the bioconversion from Dsorbitol in Gluconobacter or Acetobacter 35 . Here, we show that the rare sugar L-sorbose induces apoptotic cell death in cancer cells.…”

Molybdenum sulfide-2D nanosheets offering multiple metallic sites enable different sugar epimerization reactions to rare sugars in water

“…The former proceeds via a 1,4-hydride shift, 54 while the latter proceeds via a C2-C1 carbon shift. 15 This hydride shift in a long-range carbon is normally achieved by biological enzymes, 55 and it may be difficult to enable this specific molecular rearrangement by a chemical substance containing a single active species. In this case, in postulation, the nucleophile sulfur induces the hydride shift, while Mo has an ionic interaction and forms a metal-sugar complex (as proposed in Scheme 2).…”

Fructose Epimerization to l-Sorbose in Water over Molybdenum Oxide: Reaction Kinetics and Mechanism Insights

“…Also, it serves as a potential feedstock for another important variety of rare sugar molecule preparation, such as tagatose via epimerization. , However, as of now, the majority of its production is achieved through a biochemical pathway by utilizing a variety of sugars, including fructose, sorbitol, and glucose, through a two-stage protocol. ,, For example, when fructose is used as a substrate, sorbitol is first dehydrogenated and subsequently dehydrated to yield sorbose . Biological strains, such as Gluconobacter and Acetobacter , are shown to be promising in sorbitol production . Despite several improvements made to the biological process systems, such as the supplementation of fermentation inducers (e.g., casamino acid) to increase sorbose productivity, a yield of less than 36% is reported after prolonged incubation (>48 h). , Notwithstanding, biological processing is challenging due to difficult microbe recycling, downstream product processing, and enzyme activity loss. ,, These factors can significantly affect the economics of sorbose production, thereby limiting its application as a regular sugar substitute.…”

“…Biological strains, such as Gluconobacter and Acetobacter , are shown to be promising in sorbitol production . Despite several improvements made to the biological process systems, such as the supplementation of fermentation inducers (e.g., casamino acid) to increase sorbose productivity, a yield of less than 36% is reported after prolonged incubation (>48 h). , Notwithstanding, biological processing is challenging due to difficult microbe recycling, downstream product processing, and enzyme activity loss. ,, These factors can significantly affect the economics of sorbose production, thereby limiting its application as a regular sugar substitute. From the literature, it is chemically producible via a single-step conversion using glucose.…”

“…16 Biological strains, such as Gluconobacter and Acetobacter, are shown to be promising in sorbitol production. 17 Despite several improvements made to the biological process systems, such as the supplementation of fermentation inducers (e.g., casamino acid) 18 to increase sorbose productivity, a yield of less than 36% is reported after prolonged incubation (>48 h). 14,17 Notwithstanding, biological processing is challenging due to difficult microbe recycling, downstream product processing, and enzyme activity loss.…”