Rare sugar l-sorbose exerts antitumor activity by impairing glucose metabolism

Xu, Haitao; Zhou, Xiaoman; Chen, Shuai; Xu, Si; Li, Zijie; Nakanishi, Hideki; Gao, Xiao‐Dong

doi:10.1038/s42003-023-04638-z

Cited by 7 publications

(5 citation statements)

References 45 publications

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“…Sorbose is a monosaccharide ketose sugar (Wen et al, 2015), that limits glycolysis in some cell types, such as canine erythrocyte and murine cancer cells (Kistler and Keller, 1978;Xu et al, 2023). Additionally, in murine neoplastic cells, sorbose stimulates ROS production and contributes to apoptosis (Xu et al, 2023). In the present study, sorbose generally was limited at GV and MI, but was significantly greater in abundance at MIIC in all cell types, suggesting a possible association with culture.…”

Section: Amino Acids and Derivativessupporting

confidence: 45%

“…The function of myo-inositol in cumulus and granulosa cells requires further study. Sorbose is a monosaccharide ketose sugar (Wen et al, 2015), that limits glycolysis in some cell types, such as canine erythrocyte and murine cancer cells (Kistler and Keller, 1978;Xu et al, 2023). Additionally, in murine neoplastic cells, sorbose stimulates ROS production and contributes to apoptosis (Xu et al, 2023).…”

Section: Amino Acids and Derivativesmentioning

confidence: 99%

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Maturation and culture affect the metabolomic profile of oocytes and follicular cells in young and old mares

Bresnahan,

Catandi,

Peters

et al. 2024

Front. Cell Dev. Biol.

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Introduction: Oocytes and follicular somatic cells within the ovarian follicle are altered during maturation and after exposure to culture in vitro. In the present study, we used a nontargeted metabolomics approach to assess changes in oocytes, cumulus cells, and granulosa cells from dominant, follicular-phase follicles in young and old mares.Methods: Samples were collected at three stages associated with oocyte maturation: (1) GV, germinal vesicle stage, prior to the induction of follicle/oocyte maturation in vivo; (2) MI, metaphase I, maturing, collected 24 h after induction of maturation in vivo; and (3) MIIC, metaphase II, mature with collection 24 h after induction of maturation in vivo plus 18 h of culture in vitro. Samples were analyzed using gas and liquid chromatography coupled to mass spectrometry only when all three stages of a specific cell type were obtained from the same mare.Results and Discussion: Significant differences in metabolite abundance were most often associated with MIIC, with some of the differences appearing to be linked to the final stage of maturation and others to exposure to culture medium. While differences occurred for many metabolite groups, some of the most notable were detected for energy and lipid metabolism and amino acid abundance. The study demonstrated that metabolomics has potential to aid in optimizing culture methods and evaluating cell culture additives to support differences in COCs associated with maternal factors.

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Section: Amino Acids and Derivativessupporting

confidence: 45%

Section: Amino Acids and Derivativesmentioning

confidence: 99%

Maturation and culture affect the metabolomic profile of oocytes and follicular cells in young and old mares

Bresnahan,

Catandi,

Peters

et al. 2024

Front. Cell Dev. Biol.

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“…Although minimally studied in mammalian follicles, sorbose is a ketose belonging to the monosaccharide group of sugars ( Wen et al, 2015 ). In other cell types, such as canine erythrocytes and murine cancer cells, sorbose inhibits hexokinase, thus limiting glycolysis ( Kistler and Keller, 1978 ; Xu et al, 2023 ). In murine neoplastic cells, sorbose impairs mitochondrial function, stimulates ROS production, and attenuates cellular antioxidant defenses, ultimately contributing to apoptosis ( Xu et al, 2023 ).…”

Section: Discussionmentioning

confidence: 99%

Equine maternal aging affects the metabolomic profile of oocytes and follicular cells during different maturation time points

Catandi,

Bresnahan,

Peters

et al. 2023

Front. Cell Dev. Biol.

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Introduction: Oocyte quality and fertility decline with advanced maternal age. During maturation within the ovarian follicle, the oocyte relies on the associated somatic cells, specifically cumulus and granulosa cells, to acquire essential components for developmental capacity.Methods: A nontargeted metabolomics approach was used to investigate the effects of mare age on different cell types within the dominant, follicular-phase follicle at three time points during maturation. Metabolomic analyses from single oocytes and associated cumulus and granulosa cells allowed correlations of metabolite abundance among cell types.Results and Discussion: Overall, many of the age-related changes in metabolite abundance point to Impaired mitochondrial metabolic function and oxidative stress in oocytes and follicular cells. Supporting findings include a higher abundance of glutamic acid and triglycerides and lower abundance of ceramides in oocytes and somatic follicular cells from old than young mares. Lower abundance of alanine in all follicular cell types from old mares, suggests limited anaerobic energy metabolism. The results also indicate impaired transfer of carbohydrate and free fatty acid substrates from cumulus cells to the oocytes of old mares, potentially related to disruption of transzonal projections between the cell types. The identification of age-associated alterations in the abundance of specific metabolites and their correlations among cells contribute to our understanding of follicular dysfunction with maternal aging.

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“…In fact, in addition to fructose, GLUT5 can also transport L-sorbose, the C-3 epimer of D-fructose, into cells. This is a rare sugar that induces apoptosis after entering cancer cells through GLUT5 [194] . This suggests that the contribution of GLUT5 to normal or cancer cells may be more than related to fructose.…”

Section: Discussionmentioning

confidence: 99%

GLUT5: structure, functions, diseases and potential applications

Song¹,

Mao²,

Wei³

2023

ABBS

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Glucose transporter 5 (GLUT5) is a membrane transporter that specifically transports fructose and plays a key role in dietary fructose uptake and metabolism. In recent years, a high fructose diet has occupied an important position in the daily intake of human beings, resulting in a significant increase in the incidence of obesity and metabolic diseases worldwide. Over the past few decades, GLUT5 has been well understood to play a significant role in the pathogenesis of human digestive diseases. Recently, the role of GLUT5 in human cancer has received widespread attention, and a large number of studies have focused on exploring the effects of changes in GLUT5 expression levels on cancer cell survival, metabolism and metastasis. However, due to various difficulties and shortcomings, the molecular structure and mechanism of GLUT5 have not been fully elucidated, which to some extent prevents us from revealing the relationship between GLUT5 expression and cell carcinogenesis at the protein molecular level. In this review, we summarize the current understanding of the structure and function of mammalian GLUT5 and its relationship to intestinal diseases and cancer and suggest that GLUT5 may be an important target for cancer therapy.

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Rare sugar l-sorbose exerts antitumor activity by impairing glucose metabolism

Cited by 7 publications

References 45 publications

Maturation and culture affect the metabolomic profile of oocytes and follicular cells in young and old mares

Maturation and culture affect the metabolomic profile of oocytes and follicular cells in young and old mares

Equine maternal aging affects the metabolomic profile of oocytes and follicular cells during different maturation time points

GLUT5: structure, functions, diseases and potential applications

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