Fabrication of columns for open-tubular liquid chromatography using photopolymerization of acrylates

Eguchi, S.; Kloosterboer, J. G.; Zegers, C.P.G.; Schoenmakers, Peter J.; Tock, P.P.H.; Kraak, J.C.; Poppe, H.

doi:10.1016/s0021-9673(01)89272-4

Cited by 22 publications

(5 citation statements)

References 20 publications

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“…Much effort has been made to covalently bond a thick polymer film inside a fused-silica capillary, perhaps most notably in the work of Poppe's group. − In situ photopolymerization was used to incorporate thick polyacrylate films in 5−10 μm i.d. capillaries.…”

mentioning

confidence: 99%

Preparation and Evaluation of Bonded Linear Polymethacrylate Stationary Phases for Open Tubular Capillary Electrokinetic Chromatography

Tan

Remcho

1997

Anal. Chem.

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mentioning

confidence: 99%

Preparation and Evaluation of Bonded Linear Polymethacrylate Stationary Phases for Open Tubular Capillary Electrokinetic Chromatography

Tan

Remcho

1997

Anal. Chem.

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“…The main obstacle has been the lack of techniques for the formation of a sufficient amount of stationary phase on the capillary walls to yield a useful retention capacity. Using nonextractable stationary phases, the highest retention capacity was obtained in columns coated with a highly hydrophobic coating [13]. Such stationary films were prepared by in situ photopolymerization of a silicone acrylate and lauryl acrylate solution in transparent fused silica capillaries.…”

Section: Considerable Progress In Column Technologymentioning

confidence: 99%

“…Immobilized polysiloxanes behave mainly as liquid-like stationary phases. Attempts to overcome the relatively low retention observed on most nonextractable phases have been made through the formation of thick polymer films in OTCs [7,8,11,13]. The coating techniques available allow the deposition of stationary phase films of different thickness in 5-10-pm i.d.…”

Section: Considerable Progress In Column Technologymentioning

confidence: 99%

Narrow (5‐50‐μm i.d.) open tubular columns in liquid chromatography using immobilized polymethyloctadecylsiloxane as stationary phase

Göhlin

Larsson

1991

J Microcolumn Separations

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Abstract. This paper presents in detail the preparation of highly efficient, stable open tubular (OT) columns for reversed-phase liquid chromatography. Chromatographic properties of 5-50-pm i.d. fused silica columns (1.5-5 m in length) are described, and details of the coating procedure are discussed. On-column fluorescence detection was used for the model substituted-anthracene test solutes. Gas chromatography was used as a complementary technique for column characterization.The stationary phase studied, immobilized polymethyloctadecylsiloxane, PMSCI8, was chosen because of its more lipophilic character compared to other siloxanes. As expected, a higher retention power was found compared to polydimethylsiloxane coatings.The polymeric stationary phase showed good properties. More than 900,000 plates were achieved in 25 min (k = 0.1) in a 5-pm i.d. fused silica OT column. The stationary phase contribution to band broadening was less than 10% in an 11-pm i.d. column at a flow rate thirty times uOpt (k = 1.3).

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“…Although OT‐LC was introduced in 1978 , its use was limited by several obstacles such as extracolumn volumes, loadability, and the LODs of detectors . The loadability of OT columns was improved by introducing porous layers (PLOT columns) by etching silica and cross‐linking polymeric layers . Although OT‐LC was already combined with MS in 1989 , a renewed interest in OT‐LC began with the introduction of nano‐electrospray in combination with OT columns .…”

Section: Introductionmentioning

confidence: 99%

Separation optimization of long porous‐layer open‐tubular columns for nano‐LC–MS of limited proteomic samples

Røgeberg

Vehus

Grutle

et al. 2013

J of Separation Science

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The single-run resolving power of current 10 μm id porous-layer open-tubular (PLOT) columns has been optimized. The columns studied had a poly(styrene-co-divinylbenzene) porous layer (~0.75 μm thickness). In contrast to many previous studies that have employed complex plumbing or compromising set-ups, SPE-PLOT-LC-MS was assembled without the use of additional hardware/noncommercial parts, additional valves or sample splitting. A comprehensive study of various flow rates, gradient times, and column length combinations was undertaken. Maximum resolution for <400 bar was achieved using a 40 nL/min flow rate, a 400 min gradient and an 8 m long column. We obtained a 2.3-fold increase in peak capacity compared to previous PLOT studies (950 versus previously obtained 400, when using peak width = 2σ definition). Our system also meets or surpasses peak capacities obtained in recent reports using nano-ultra-performance LC conditions or long silica monolith nanocolumns. Nearly 500 proteins (1958 peptides) could be identified in just one single injection of an extract corresponding to 1000 BxPC3 beta catenin (-/-) cells, and ~1200 and 2500 proteins in extracts of 10,000 and 100,000 cells, respectively, allowing detection of central members and regulators of the Wnt signaling pathway.

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Fabrication of columns for open-tubular liquid chromatography using photopolymerization of acrylates

Cited by 22 publications

References 20 publications

Preparation and Evaluation of Bonded Linear Polymethacrylate Stationary Phases for Open Tubular Capillary Electrokinetic Chromatography

Preparation and Evaluation of Bonded Linear Polymethacrylate Stationary Phases for Open Tubular Capillary Electrokinetic Chromatography

Narrow (5‐50‐μm i.d.) open tubular columns in liquid chromatography using immobilized polymethyloctadecylsiloxane as stationary phase

Separation optimization of long porous‐layer open‐tubular columns for nano‐LC–MS of limited proteomic samples

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