Preparation and Evaluation of Bonded Linear Polymethacrylate Stationary Phases for Open Tubular Capillary Electrokinetic Chromatography

Tan, Zhixin Jessica; Remcho, Vincent T.

doi:10.1021/ac960894z

Cited by 81 publications

(46 citation statements)

References 12 publications

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“…: 186-411-369-3409; fax: 186-411-gradually to include textile dyes, food coloring, stationary phases used in CEC also have expanded to chemical grade, and the other reagents used were of C derivatized silica, ion-exchange packings, chiral the analytical reagent grade. Ultra-pure water used 8 materials and various polymer phases [5,7,[13][14][15][16][17][18]. for preparation of solutions was produced by a MilliRecently, a specially designed stationary phase for Q water system (Millipore, Bedford, MA, USA).…”

Section: Introductionmentioning

confidence: 99%

Separation of peptides by strong cation-exchange capillary electrochromatography

Zou

Liu

et al. 2000

Journal of Chromatography A

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Section: Introductionmentioning

confidence: 99%

Separation of peptides by strong cation-exchange capillary electrochromatography

Zou

Liu

et al. 2000

Journal of Chromatography A

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“…Earlier studies [4][5][6][7][8] have demonstrated that the fabrication of thermally initiated PLOT type columns is possible, however, stationary phase layers could only be obtained in capillaries of small ID (typically less than 25 m) where they were successfully applied to capillary LC [5], but these dimensions are too small for application in GC. Chuang et al [9] has reported the thermal polymerisation of a polystyrene 'monolithic' layer inside a 75 m ID capillary, yet the layer thickness achieved was only 300 nm with little or no porosity.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Bonded Monolithic Porous Layer Open Tubular (monoPLOT) Columns in Wide Bore Capillary by Laminar Flow Thermal Initiation

et al. 2013

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A novel scalable procedure for the thermally initiated polymerisation of bonded monolithic porous layers of controlled thickness within open tubular fused silica capillaries (monoPLOT columns) is presented. Porous polymer layers of either polystyrenedivinylbenzene or butyl methacrylate-ethylene dimethacrylate, of variable thickness and morphology were polymerised inside fused silica capillaries utilising combined thermal initiation and laminar flow of the polymerisation mixture. The procedure enables the production through thermal initiation of monoPLOT columns of varying length, internal diameter, user defined morphology and layer thickness for potential use in both liquid and gas chromatography. The morphology and thickness of the bonded polymer layer on the capillary wall is strongly dependent on the laminar flow properties of the polymerisation mixture and the changing shear stress within the fluid across the inner diameter of the open capillary. Owing to the highly controlled rate of polymerisation and its dependence on fluid shear stress at the capillary wall, the procedure was demonstrably scalable, as illustrated by the polymerisation of identical layers within different capillary diameters.

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“…Several techniques for the manufacture of PLOT columns exist [1][2][3][4][5][6][7][8][9] and include both thermal and photo-initiated methods. However, in the majority of these techniques, it was difficult to precisely control layer thickness and morphology.…”

Section: Introductionmentioning

confidence: 99%

In-process phase growth measurement technique in the fabrication of monolithic porous layer open tubular (monoPLOT) columns using capacitively coupled contactless conductivity

Collins

Nesterenko

Brabazon

et al. 2013

Analyst

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A technique for the in-process measurement of polymer stationary phase growth inside fused silica capillaries during the fabrication of monolithic porous layer open tubular (monoPLOT) columns is presented. In this work, capacitively coupled contactless conductivity detection (C4D) was applied as an online measurement tool for porous polymer layer growth within fused silica capillaries. The relationship between effective capillary diameter and C4D response was investigated for two polymers, butyl methacrylate-ethylene dimethacrylate (BuMA-EDMA) and polystyrene-divinylbenzene (PS-DVB) over a range of capillary diameters and layer thicknesses. The described technique can be used with both thermal and photo-initiated approaches for monoPLOT fabrication and provides an accurate, realtime measurement of the porous layer growth within the capillary, which should vastly improve columnto-column reproducibility. The technique was shown to be very precise, with a measured %RSD < 10%.

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Preparation and Evaluation of Bonded Linear Polymethacrylate Stationary Phases for Open Tubular Capillary Electrokinetic Chromatography

Cited by 81 publications

References 12 publications

Separation of peptides by strong cation-exchange capillary electrochromatography

Separation of peptides by strong cation-exchange capillary electrochromatography

Fabrication of Bonded Monolithic Porous Layer Open Tubular (monoPLOT) Columns in Wide Bore Capillary by Laminar Flow Thermal Initiation

In-process phase growth measurement technique in the fabrication of monolithic porous layer open tubular (monoPLOT) columns using capacitively coupled contactless conductivity

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