Fabrication, characterization, and <i>in vitro</i> evaluation of silver‐containing arabinoxylan foams as antimicrobial wound dressing

Aduba, Donald C.; An, Seon Sook; Selders, Gretchen S.; Wang, Juan; Yeudall, W. Andrew; Bowlin, Gary L.; Kitten, Todd

doi:10.1002/jbm.a.35783

Cited by 12 publications

(10 citation statements)

References 35 publications

(70 reference statements)

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“…A similar behavior was reported by Samuelsen et al [46] where the treatment of AX from barley (0.5–3 mg/mL AX) had no significant effect on the proliferation of two human colon cancer cell lines, HT-29 and Caco-2. Moreover, in a previous study, AX foams prepared by peroxidase/H 2 O 2 enzymatic cross-linking reaction did not have a cytotoxic effect on NIH3T3 fibroblasts [67]. In that study, the cell viability values (~96%) were close to those observed in the non-cancerous cell line (CCD 841 CoN) treated with AXM, which was evaluated in the present study.…”

Section: Discussionsupporting

confidence: 72%

Arabinoxylan-Based Particles: In Vitro Antioxidant Capacity and Cytotoxicity on a Human Colon Cell Line

Méndez-Encinas¹,

Carvajal-Millán²,

Rascón‐Chu³

et al. 2019

Medicina

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Background and objectives: Arabinoxylans (AX) can gel and exhibit antioxidant capacity. Previous studies have demonstrated the potential application of AX microspheres as colon-targeted drug carriers. However, the cytotoxicity of AX gels has not been investigated so far. Therefore, the aim of the present study was to prepare AX-based particles (AXM) by coaxial electrospraying method and to investigate their antioxidant potential and cytotoxicity on human colon cells. Materials and Methods: The gelation of AX was studied by monitoring the storage (G′) and loss (G′′) moduli. The morphology of AXM was evaluated using optical and scanning electron microscopy (SEM). The in vitro antioxidant activity of AX before and after gelation was measured using the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods. In addition, the effect of AX and AXM on the proliferation of human colon cells (CCD 841 CoN) was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: The final G′ and G′′ values for AX gels were 293 and 0.31 Pa, respectively. AXM presented spherical shape and rough surface with a three-dimensional and porous network. The swelling ratio and mesh size of AXM were 35 g water/g AX and 27 nm, respectively. Gelation decreased the antioxidant activity of AX by 61–64 %. AX and AXM did not affect proliferation or show any toxic effect on the normal human colon cell line CCD 841 CoN. Conclusion: The results indicate that AXM could be promising biocompatible materials with antioxidant activity.

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Section: Discussionsupporting

confidence: 72%

Arabinoxylan-Based Particles: In Vitro Antioxidant Capacity and Cytotoxicity on a Human Colon Cell Line

Méndez-Encinas¹,

Carvajal-Millán²,

Rascón‐Chu³

et al. 2019

Medicina

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“…2d). The hydrogel's antibacterial properties could be attributed to the reversible Ag-S bond produced from crosslinking SH-PEG and AgNO 3 and the hydrogel's interaction with sulfur-containing proteins in the bacterial cell membrane 26,27 . The enhanced prevention of bacterial infections for wound healing was evident in the DFO-hydrogel's measured inhibition zone, which was larger in comparison to that of the hydrogel.…”

Section: Resultsmentioning

confidence: 99%

An injectable self-healing coordinative hydrogel with antibacterial and angiogenic properties for diabetic skin wound repair

et al. 2019

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We report here an injectable, self-healing coordinative hydrogel with antibacterial and angiogenic properties for diabetic wound regeneration. The hydrogel was prepared by coordinative cross-linking of multi-arm thiolated polyethylene glycol (SH-PEG) with silver nitrate (AgNO3). Due to the dynamic nature of Ag-S coordination bond and bacteria-killing activity of Ag+, the resultant coordinative hydrogel featured self-healing, injectable and antibacterial properties. In this study, we synchronously loaded an angiogenic drug, desferrioxamine (DFO), in the coordinative hydrogel during cross-linking. We finally obtained a multifunctional hydrogel that is manageable, resistant to mechanical irritation, antibacterial and angiogenic in vitro. Our in vivo studies further demonstrated that the injectable self-healing hydrogel could efficiently repair diabetic skin wounds with low bacteria-infection and enhance angiogenic activity. In short, besides diabetic skin wound repair, such dynamic multifunctional hydrogel scaffolds would show great promise in the regeneration of different types of exposed wounds, in particular, in situations with disturbed physiological functions, high risk of bacterial infections, and external mechanical irritation.

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“…Experiments revealed arabinoxylan hydrogels to have a two-and-a-half-fold increase in swelling when introduced into water [ 43 ]. Lyophilized arabinoxylan gels fabricated in our study have demonstrated swelling ratios above 20 after 48 h [ 44 ]. Arabinoxylan is an intriguing polysaccharide for hydrogel wound dressing formation because it does not require the use of toxic organic solvents but instead uses water instead for solubilizing before crosslinking.…”

Section: Fabrication Platforms Of Polysaccharides As Wound Dressinmentioning

confidence: 99%

“…The study showed complete dermal regeneration after implantation of dextran hydrogels compared to non-treated and treated control groups. Our group has developed lyophilized arabinoxylan hydrogels that exhibited viabilities above 96% on fibroblasts cultured in vitro [ 44 ]. The results concluded that fibroblasts that play a prominent role in the proliferative and remodeling stages of wound healing maintain their cytocompatibility after exposure to arabinoxylan.…”

Section: Biocompatibility Assessment Of Polysaccharides As Wound Dmentioning

confidence: 99%

“…Crosslinked arabinoxylan gels have high water absorption capacity that enables potential drug delivery applications using therapeutics such as albumin and ibuprofen [ 41 , 106 ]. Our previous work demonstrated arabinoxylan foams impregnated with silver can effectively serve as an in vitro antimicrobial wound dressing material [ 44 ]. Alginate-pectin aerogel particles with pectin core shells have encapsulated doxycycline antibiotic in wound dressings for sustained drug release against tissue degrading MMPs during chronic wound healing [ 107 ].…”

Section: Biocompatibility Assessment Of Polysaccharides As Wound Dmentioning

confidence: 99%

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Polysaccharide Fabrication Platforms and Biocompatibility Assessment as Candidate Wound Dressing Materials

Aduba

Yang

2017

Bioengineering

Self Cite

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Wound dressings are critical for wound care because they provide a physical barrier between the injury site and outside environment, preventing further damage or infection. Wound dressings also manage and even encourage the wound healing process for proper recovery. Polysaccharide biopolymers are slowly becoming popular as modern wound dressings materials because they are naturally derived, highly abundant, inexpensive, absorbent, non-toxic and non-immunogenic. Polysaccharide biopolymers have also been processed into biomimetic platforms that offer a bioactive component in wound dressings that aid the healing process. This review primarily focuses on the fabrication and biocompatibility assessment of polysaccharide materials. Specifically, fabrication platforms such as electrospun fibers and hydrogels, their fabrication considerations and popular polysaccharides such as chitosan, alginate, and hyaluronic acid among emerging options such as arabinoxylan are discussed. A survey of biocompatibility and bioactive molecule release studies, leveraging polysaccharide’s naturally derived properties, is highlighted in the text, while challenges and future directions for wound dressing development using emerging fabrication techniques such as 3D bioprinting are outlined in the conclusion. This paper aims to encourage further investigation and open up new, disruptive avenues for polysaccharides in wound dressing material development.

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Fabrication, characterization, and in vitro evaluation of silver‐containing arabinoxylan foams as antimicrobial wound dressing

Cited by 12 publications

References 35 publications

Arabinoxylan-Based Particles: In Vitro Antioxidant Capacity and Cytotoxicity on a Human Colon Cell Line

Arabinoxylan-Based Particles: In Vitro Antioxidant Capacity and Cytotoxicity on a Human Colon Cell Line

An injectable self-healing coordinative hydrogel with antibacterial and angiogenic properties for diabetic skin wound repair

Polysaccharide Fabrication Platforms and Biocompatibility Assessment as Candidate Wound Dressing Materials

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