An injectable self-healing coordinative hydrogel with antibacterial and angiogenic properties for diabetic skin wound repair

Abstract

We report here an injectable, self-healing coordinative hydrogel with antibacterial and angiogenic properties for diabetic wound regeneration. The hydrogel was prepared by coordinative cross-linking of multi-arm thiolated polyethylene glycol (SH-PEG) with silver nitrate (AgNO3). Due to the dynamic nature of Ag-S coordination bond and bacteria-killing activity of Ag+, the resultant coordinative hydrogel featured self-healing, injectable and antibacterial properties. In this study, we synchronously loaded an angiogenic drug, desferrioxamine (DFO), in the coordinative hydrogel during cross-linking. We finally obtained a multifunctional hydrogel that is manageable, resistant to mechanical irritation, antibacterial and angiogenic in vitro. Our in vivo studies further demonstrated that the injectable self-healing hydrogel could efficiently repair diabetic skin wounds with low bacteria-infection and enhance angiogenic activity. In short, besides diabetic skin wound repair, such dynamic multifunctional hydrogel scaffolds would show great promise in the regeneration of different types of exposed wounds, in particular, in situations with disturbed physiological functions, high risk of bacterial infections, and external mechanical irritation.

Adhesive liposomes loaded onto an injectable, self-healing and antibacterial hydrogel for promoting bone reconstruction

“…The FTIR spectra collected from the A-LIP-PEG samples had vibration signals from the disulfide bonds (ν(S-S), 530 cm −1 ) (Fig. 3d) 41 . SH was prone to forming disulfide bonds, resulting in hydrogel formation.…”

“…The mechanism of self-healing might be that A-LIP-PEG was crosslinked by the coordination of Ag-S and disulfide bonds (Fig. 2j) 41 . When the A-LIP-PEG was cut, the bonds were broken, and when they came into contact with each other, the A-LIP-PEG was restored.…”

“…SH was prone to forming disulfide bonds, resulting in hydrogel formation. The coordination between Ag and S and the disulfide bond were two ways that the lipo-hydrogel formed 41 . A-LIP-PEG reached swelling equilibrium at 24 h, and the swelling ratio reached 1070 ± 140%; LIP-PEG reached swelling equilibrium at 24 h, and the swelling ratio reached 1000 ± 150% (Fig.…”

“…This performance was helpful for maintaining the drug concentration, inhibiting the dilution of the drug in the body fluids and allowing targeting and localization of a drug to a specific site 45 . To study an appropriate clinical treatment model of infection, S. aureus and Escherichia coli were adopted as representative Gram-positive and Gram-negative bacteria, respectively, and they were used to evaluate the antibacterial ability of our lipo-hydrogels 41 . At present, after clinical fracture surgery, there was still a potential risk of bacterial infection, such as improper operation procedures by medical staff and improper activity of patients.…”

Dual functional β-peptide polymer-modified resin beads for bacterial killing and endotoxin adsorption

“…Endotoxins can activate complex immune effectors to generate a hyperinflammatory response and even provoke severe endotoxic shock and multiorgan dysfunction [14][15][16][17][18]. Therefore, multifunctional antibacterial materials are highly desirable for both efficient bacterial killing and biosafety considerations [19][20][21][22][23][24][25].…”