Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolate Possessing the Shiga Toxin Gene (
            <i>stx</i>
            <sub>1</sub>
            ) Belonging to the O64 Serogroup Associated with Human Disease in India

Dutta, T. K.; Warjri, Iadarilin; Roychoudhury, P.; Lalzampuia, H.; Samanta, Indranil; Joardar, S. N.; Bandyopadhyay, S.; Chandra, Rajesh

doi:10.1128/jcm.00575-13

Cited by 25 publications

(28 citation statements)

References 24 publications

(12 reference statements)

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“…Thus, the exact burden of E. coli diarrhea among the hospitalized children across India especially Northeast India is still unclear. In the present study, the samples were collected from Mizoram, located in the North Eastern part of India adjoining to Myanmar and Bangladesh where limited study has been conducted on the prevalence of diarrhea associated with DEC [15, 21]. This is the first report on an in-depth study on the prevalence of EPEC and EIEC associated with diarrhea in children less than 5 years of age in North-eastern Indian population.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of multi drug resistant enteropathogenic and enteroinvasive Escherichia coli isolated from children with and without diarrhea in Northeast Indian population

Chellapandi

Dutta

Sharma

et al. 2017

Ann Clin Microbiol Antimicrob

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BackgroundDiarrheagenic Escherichia coli are associated with infantile diarrhea in the developing countries. The present study was conducted to determine the occurrence and antimicrobial resistance pattern of enteropathogenic and enteroinvasive E. coli associated with diarrhoea among the paediatric patients.MethodsA total of 262 stool samples were collected from children with and without diarrhea from Mizoram, Northeast India. E. coli were isolated and subjected to multiplex PCR to detect virulent genes of EPEC (eaeA and bfpA) and EIEC (ial). Isolates were subjected to antimicrobial sensitivity assay using disc diffusion method. Selected eaeA genes were sequenced for identification and genetic relationship.ResultsA total of 334 E. coli was isolated, of which 17.37% were carrying at least one virulent gene. Altogether, 14.97 and 2.40% isolates were categorized as EPEC and EIEC, respectively. Among the DEC isolates, 4.79% were EPEC and 7.78% were EIEC. A total of 8 (2.40%) isolates were EIEC (ial+), of which 6 (1.80%) and 2 (0.60%) were from diarrhoeic and non-diarrhoeic patients, respectively. A total of 24 (41.40%) DEC isolates were MDR (resistance against ≥5 antimicrobials).ConclusionsA high frequency of EPEC pathotypes associated with paediatric diarrhea was observed in Mizoram, Northeast India and majority of the isolates are resistant to antibiotics with a high frequency of MDR, which is a matter of concern to the public health. This also raises an alarm to the world communities to monitor the resistance pattern and analyse in a global scale to combat the problems of resistance development.

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Section: Discussionmentioning

confidence: 99%

Prevalence of multi drug resistant enteropathogenic and enteroinvasive Escherichia coli isolated from children with and without diarrhea in Northeast Indian population

Chellapandi

Dutta

Sharma

et al. 2017

Ann Clin Microbiol Antimicrob

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“…Ahmed and Shimamoto reported the presence of plasmid‐mediated quinolone resistance genes, qnr B and qnr S, in 12.0% of O157 isolates from meat products in Egypt (Ahmed & Shimamoto, ). Recent studies reported several β‐lactamase genes, such as bla CTX‐M , bla AmpC , bla CMY‐1 , bla TEM and bla DHA‐1 in STEC serotypes O26:H11, O64, O91:H14, O106:H18, O111:H8 and O157, recovered from humans and animals; some of these β‐lactamase genes were encoded on plasmids (Arvand et al, ; Dutta et al, ; Ishii et al, ; Iweriebor et al, ; Jost et al, ; Valat et al, ). Seven O157 isolates harbouring bla CMY‐2 , obtained from clinical samples at the Osaka Prefecture, Japan, had antibiotic resistance genes encoded on a IncI1 plasmid that has high transmissibility across bacterial species (Kawahara et al, ).…”

Section: Acquisition and Transmission Of Antibiotic Resistance In Ar‐mentioning

confidence: 99%

“…Ahmed and Shimamoto reported the presence of plasmidmediated quinolone resistance genes, qnrB and qnrS, in 12.0% of O157 isolates from meat products in Egypt (Ahmed & Shimamoto, 2015). Recent studies reported several β-lactamase genes, such as bla CTX-M , bla AmpC , bla CMY-1 , bla TEM and bla DHA-1 in STEC serotypes O26:H11, O64, O91:H14, O106:H18, O111:H8 and O157, recovered from humans and animals; some of these β-lactamase genes were encoded on plasmids (Arvand et al, 2015;Dutta et al, 2013;Ishii et al, 2005;Iweriebor et al, 2015;Jost et al, 2016;Valat et al, 2012).…”

Section: Acqu Is Iti On and Tr An S Miss I On Of Antib I Oti C Re Smentioning

confidence: 99%

Antibiotic‐resistant Shiga toxin‐producing Escherichia coli: An overview of prevalence and intervention strategies

Mir

Kudva

2018

Zoonoses and Public Health

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Shiga toxin‐producing Escherichia coli (STEC) are foodborne pathogens that can cause severe diseases, including bloody diarrhoea and kidney failure, in humans, while remaining harmless to its primary reservoir hosts, cattle. Antibiotics such as azithromycin, fosfomycin and meropenem are being used and recommended in the treatment of early‐stage STEC (mainly E. coli O157:H7) infections, as these are reportedly effective in preventing Shiga toxin release and kidney failure while eliminating the pathogen. However, antibiotic resistance among STEC isolates could negatively impact these and other similar treatment options while contributing towards the spread of antibiotic resistance genes especially if encoded on mobile genetic elements like plasmids. Antibiotic resistance among STEC isolates recovered from animals and patients is being reported globally. A comprehensive understanding of the prevalence of antibiotic‐resistant STEC (AR‐STEC) and the mechanisms promoting this resistance among these bacteria could help direct therapies and develop strategies to effectively reduce/eliminate these pathogens. Here, we have reviewed literature from the past three decades to gain insights on this prevalence and its impact on human infections. In addition, we have reviewed various strategies proposed by researchers to control STEC that in turn would be applicable to AR‐STEC as well.

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“…The major reservoir of the STEC strains in the nature is the intestinal content of cattle and other small ruminants (Kaper et al ). The occurrence of STEC are also detected in pigs, poultry, soil and water (Diallo et al ; Dutta et al ; Mandakini ; Kusumoto et al ; Kylla et al ).…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, resistance may be promoted in STEC commensally present in the intestinal tracts of animals and humans (Ramachandra et al ; King et al ). Although, STEC isolates are not considered as reservoir of extended spectrum β‐lactamases (ESBLs), a handful of reports have been published on the association of STEC isolates with ESBL/beta‐lactamase genes (Ishii et al ; Buvens et al ; Valat et al , ; Dutta et al ; Torpdahl et al ; Lupindu et al ; Nagy et al ; Mandakini ; Kusumoto et al ).…”

Section: Introductionmentioning

confidence: 99%

Extended spectrum beta‐lactamase producing Shiga‐toxin producing‐Escherichia coliin piglets, humans and water sources in North East region of India

Puii

Dutta

Roychoudhury

et al. 2019

Lett Appl Microbiol

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Significance and Impact of the Study: Multidrug resistant (MDR) enteric bacteria are global concern. Association of MDR traits in STEC isolates are another rising issue in human and animal health perspective. The interaction of such organisms among the human, domestic animals and adjoining water sources require to be analysed systematically. The present study exhibited the possible transmission of MDR-STEC among the human, domestic animals and water sources in the North eastern states of India. To the best of our knowledge, this is the first report of such kind in India. AbstractExtended spectrum b-lactamases (ESBL) producing Shiga toxin producing E. coli (STEC) are posing a constant threat to public health throughout the world leading to serious infections and raising key therapeutic issues. A total of 219 fecal samples were collected from piglets with diarrheoa, pig farmers and water sources in North East India; and were processed for isolation of Escherichia coli. The isolates were screened for antimicrobial resistance and suspected isolates for ESBLs production by double-disk synergy test (DDST). Escherichia coli isolates positive for DDST were subjected for detection of selected ESBL/beta-lactamase genes and virulence associated genes by PCR. By DDST, 337 (67Á94%) E. coli isolates were detected as ESBLs producer, of which 211 (66Á98%), 117 (70Á91%) and 9 (56Á25%) isolates were from piglets, humans and water sources respectively. A total of 64 (12Á90%) isolates were recorded as STEC, of which 48 (9Á68%), 6 (1Á21%) and 10 (2Á02%) were from human, piglets and water respectively. Majority of the STEC isolates (64Á06%) possessed multiple virulence genes, of which 59Á38% also harboured ESBL/ beta-lactamase genes with 32Á81% STEC isolates being positive for multiple ESBL/beta-lactamase genes.Letters in Applied Microbiology ISSN 0266-8254

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Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolate Possessing the Shiga Toxin Gene ( stx ₁ ) Belonging to the O64 Serogroup Associated with Human Disease in India

Cited by 25 publications

References 24 publications

Prevalence of multi drug resistant enteropathogenic and enteroinvasive Escherichia coli isolated from children with and without diarrhea in Northeast Indian population

Prevalence of multi drug resistant enteropathogenic and enteroinvasive Escherichia coli isolated from children with and without diarrhea in Northeast Indian population

Antibiotic‐resistant Shiga toxin‐producing Escherichia coli: An overview of prevalence and intervention strategies

Extended spectrum beta‐lactamase producing Shiga‐toxin producing‐Escherichia coliin piglets, humans and water sources in North East region of India

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Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolate Possessing the Shiga Toxin Gene ( stx 1 ) Belonging to the O64 Serogroup Associated with Human Disease in India

Cited by 25 publications

References 24 publications

Prevalence of multi drug resistant enteropathogenic and enteroinvasive Escherichia coli isolated from children with and without diarrhea in Northeast Indian population

Prevalence of multi drug resistant enteropathogenic and enteroinvasive Escherichia coli isolated from children with and without diarrhea in Northeast Indian population

Antibiotic‐resistant Shiga toxin‐producing Escherichia coli: An overview of prevalence and intervention strategies

Extended spectrum beta‐lactamase producing Shiga‐toxin producing‐Escherichia coliin piglets, humans and water sources in North East region of India

Contact Info

Product

Resources

About

Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolate Possessing the Shiga Toxin Gene ( stx ₁ ) Belonging to the O64 Serogroup Associated with Human Disease in India