Antibiotic‐resistant Shiga toxin‐producing <i>Escherichia coli</i>: An overview of prevalence and intervention strategies

Mir, Raies A.; Kudva, Indira T.

doi:10.1111/zph.12533

Cited by 58 publications

(59 citation statements)

References 139 publications

(243 reference statements)

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“…E. coli O157: H7 AC188 was less susceptible to STP than ATCC 43888; this is correlated with previous reports that have shown the development of resistance of clinical isolates to aminoglycosides because of indiscriminate use of antimicrobials [10,26]. Unlike that observed with STP, the Ib-M peptides showed similar MICs in both strains of E. coli O157: H7.…”

Section: Discussionsupporting

confidence: 88%

“…To extend the previous findings on the antimicrobial activity of the Ib-M peptides, this work evaluated its antibacterial properties against E. coli O157: H7, the most prevalent serotype of shigatoxigenic E. coli (STEC), a well-established bacterial foodborne pathogen, whose overall incidence is estimated in approximately 2.8 million cases of acute gastrointestinal disease annually [8]. The STEC-induced illness is characterized by bloody diarrhea, hemorrhagic colitis, and complications associated with the development of hemolytic uremic syndrome [9,10].…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7

Prada-Prada¹,

Flórez-Castillo

Farfán-García³

et al. 2020

PLoS ONE

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The development of new antimicrobial peptides has become an attractive alternative to conventional antibiotics due to the increasing rates of microbial drug resistance. Ib-M corresponds to a family of cationic synthetic peptides, 20 amino acids in length, that have shown inhibitory effect against the non-pathogenic strain Escherichia coli K-12. This work evaluated the antimicrobial potential of Ib-M peptides against the pathogenic E. coli O157: H7 using a reference strain and a clinical isolate. The Ib-M peptides showed antibacterial activity against both strains of E. coli O157: H7; the minimum inhibitory concentration of Ib-M peptides ranged from 1.6 to 12.5 μM and the minimum bactericidal concentration ranged from 3.7 to 22.9 μM, being Ib-M1 and Ib-M2 the peptides that presented the highest inhibitory effect. Time-kill kinetics assay showed a reduction of the bacterial population by more than 95% after 4 hours of exposure to 1xMIC of Ib-M1. Low cytotoxicity was observed in VERO cells with 50% cytotoxic concentration in the range from 197.5 to more than 400 μM. All peptides showed a random structure in hydrophilic environments, except Ib-M1, and all of them transitioned to an α-helical structure when the hydrophobicity of the medium was increased. In conclusion, these findings support the in vitro antimicrobial effect of Ib-M peptides against the pathogenic bacteria E. coli O157: H7 and prove to be promising molecules for the development of new therapeutic alternatives.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7

Prada-Prada¹,

Flórez-Castillo

Farfán-García³

et al. 2020

PLoS ONE

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“…Studies have shown that individuals with a low bacterial richness are characterized by a more inflammatory phenotype [56]. The association between increased Enterobacter and decreased renal function has been confirmed in many studies [57,58]. The mechanisms behind these phenomena may be attributable to the inflammatory immune response and intestinal susceptibility of IgAN.…”

Section: Discussionmentioning

confidence: 89%

Fecal microbiota characteristics of Chinese patients with primary IgA nephropathy: a cross-sectional study

Xie

et al. 2020

BMC Nephrol

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Background: Growing evidence has shown that the gut-renal connection and gut microbiota dysbiosis play a critical role in immunoglobulin A nephropathy (IgAN). However, the fecal microbiome profile in Chinese patients with IgAN remains unknown. A cross-sectional study was designed for the first time to investigate the fecal microbiota compositions in patients with primary IgAN in China and to evaluate the relationship between the fecal microbiome and IgAN clinical presentation. Methods: Fecal samples were collected from 17 IgAN patients and 18 age-, sex-, and body mass index-matched healthy controls, and bacterial DNA was extracted for 16S ribosomal RNA gene sequencing targeting the V3-V4 region. Results: Fecal samples from the IgAN patients and healthy controls showed differences in gut microbiota community richness and compositions. Compared to the healthy controls, IgAN patients at the phylum level had an increased abundance of Fusobacteria, but a decreased abundance of Synergistetes. The significantly increased genera in the IgAN group were Escherichia-Shigella, Hungatella, and Eggerthella, all of which possess pathogenic potential. Furthermore, the genus Escherichia-Shigella was negatively associated with the estimated glomerular filtration rate (eGFR) but was positively associated with the urinary albumin-to-creatinine ratio (uACR). However, the genus rectale_group was present in the IgAN group with a low abundance and was negatively associated with the uACR. Functional analysis disclosed that infection-related pathways were enriched in the IgAN group. Conclusions: We demonstrate that gut microbiota dysbiosis occurs in patients with IgAN, and that changes in gut bacterial populations are closely related to IgAN clinical features, suggesting that certain specific gut microbiota may be a potential therapeutic target for IgAN.

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“…In addition, although antibiotics are not used to clear STEC in cattle, the presence of AR genes in STEC could contribute towards increased dissemination of antibiotic resistance in the environment. Several studies in the US and abroad have reported resistance to aminoglycosides, β-lactams, carbapenems, cephalosporins, erythromycin, phenicols, streptomycin, sulpha-drugs, and tetracyclines, besides multidrug resistance in STEC isolated from humans and animals [31,76,[79][80][81][82]. Meta-analysis of the AR data for the six control O157, as annotated in the Pathosystems Resource Integration Center (PATRIC) Bioinformatics Resource Center (https://www.…”

Section: Discussionmentioning

confidence: 99%

Supershed Escherichia coli O157:H7 Has Potential for Increased Persistence on the Rectoanal Junction Squamous Epithelial Cells and Antibiotic Resistance

Mir

Brunelle

Alt

et al. 2020

International Journal of Microbiology

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Supershedding cattle shed Escherichia coli O157:H7 (O157) at ≥ 104 colony-forming units/g feces. We recently demonstrated that a supershed O157 (SS-O157) strain, SS-17, hyperadheres to the rectoanal junction (RAJ) squamous epithelial (RSE) cells which may contribute to SS-O157 persistence at this site in greater numbers, thereby increasing the fecal O157 load characterizing the supershedding phenomenon. In order to verify if this would be the signature adherence profile of any SS-O157, we tested additional SS-O157 isolates (n = 101; each from a different animal) in the RSE cell adherence assay. Similar to SS-17, all 101 SS-O157 exhibited aggregative adherence on RSE cells, with 56% attaching strongly (>10 bacteria/cell; hyperadherent) and 44% attaching moderately (1–10 bacteria/cells). Strain typing using Polymorphic Amplified Typing Sequences (PATS) analysis assigned the 101 SS-O157 into 5 major clades but not to any predominant genotype. Interestingly, 69% of SS-O157 isolates were identical to human O157 outbreak strains based on pulsed field gel electrophoresis profiles (CDC PulseNet Database), grouped into two clades by PATS distinguishing them from remaining SS-O157, and were hyperadherent on RSE cells. A subset of SS-O157 isolates (n = 53) representing different PATS and RSE cell adherence profiles were analyzed for antibiotic resistance (AR). Several SS-O157 (30/53) showed resistance to sulfisoxazole, and one isolate was resistant to both sulfisoxazole and tetracycline. Minimum inhibitory concentration (MIC) tests confirmed some of the resistance observed using the Kirby–Bauer disk diffusion test. Each SS-O157 isolate carried at least 10 genes associated with AR. However, genes directly associated with AR were rarely amplified: aac (3)-IV in 2 isolates, sul2 in 3 isolates, and tetB in one isolate. The integrase gene, int, linked with integron-based AR acquisition/transmission, was identified in 92% of SS-O157 isolates. Our results indicate that SS-O157 isolates could potentially persist longer at the bovine RAJ but exhibit limited resistance towards clinical antibiotics.

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Antibiotic‐resistant Shiga toxin‐producing Escherichia coli: An overview of prevalence and intervention strategies

Cited by 58 publications

References 139 publications

Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7

Antimicrobial activity of Ib-M peptides against Escherichia coli O157: H7

Fecal microbiota characteristics of Chinese patients with primary IgA nephropathy: a cross-sectional study

Supershed Escherichia coli O157:H7 Has Potential for Increased Persistence on the Rectoanal Junction Squamous Epithelial Cells and Antibiotic Resistance

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