Expression of TGF-β1, SNAI1 and MMP-9 is associated with lymph node metastasis in papillary thyroid carcinoma

Abstract

TGF-β1, SNAI1 and MMP-9 are implicated in tumor invasion and metastasis. The purpose of this study was to examine TGF-β1, SNAI1 and MMP-9 expression in papillary thyroid carcinoma (PTC), and to assess association of TGF-β1, SNAI1 and MMP-9 expression with several clinicopathological indicators of PTC. TGF-β1, SNAI1 and MMP-9 protein expression in 83 PTCs and their matched normal thyroid specimens were analyzed using immunohistochemistry. The mRNA expression levels of TGF-β1, SNAI1 and MMP-9 in 12 fresh PTC specimens with lymph node metastasis (LNM), 12 fresh PTC specimens without LNM and their matched normal thyroid specimens were assessed by real-time RT-PCR. The results showed that the mRNA and protein expression levels of TGF-β1, SNAI1 and MMP-9 were significantly higher in PTCs than in their matched normal thyroid tissues. There were not significant differences in TGF-β1, SNAI1 and MMP-9 protein expression relative to age, gender, tumor size and TNM stage, except for MMP-9 whose protein expression correlated with tumor size. However, high mRNA and protein expression levels of TGF-β1, SNAI1 and MMP-9 were significantly correlated with LNM. Furthermore, TGF-β1, SNAI1 and MMP-9 protein expression were significantly correlated with one another. Concomitant expression of any two or all of the three molecules had stronger correlation with LNM than did each alone. Collectively, the present results indicate that immunohistochemical and real-time RT-PCR evaluation of TGF-β1, SNAI1 and MMP-9 expression in PTC may be useful to predict the risk of LNM in PTC patients.

Tinospora crispa extract inhibits MMP-13 and migration of head and neck squamous cell carcinoma cell lines

“…Finally, the results confirmed a higher expression of MMP-13 by metastatic cell lines compared to the primary cell line. The latter finding is consistent with studies which showed that MMP-13 expression was significantly increased in tumors from lymph node metastases in head and neck squamous cell carcinomas and papillary thyroid carcinomas patients [11,24] .…”

“…Numerous findings presented in the literature demonstrated a role for MMP-13 in cell migration. MMP-13 silencing was shown to decrease tumor cell migration, whereas over-expression of MMP-13 increased cell migration and promoted metastasis [8,24]. Whether such a role of MMP-13 also counts for the cell types we have used, needs additional experiments.…”

Sorafenib in the treatment of thyroid cancer

“…It has been suggested that EGFR is highly expressed in aggressive PTC with lymph node metastasis and plays a role in the progression of TC [37], but these results are in contrast with the findings of other studies [38].…”

Fructus phyllanthi tannin fraction induces apoptosis and inhibits migration and invasion of human lung squamous carcinoma cells in vitro via MAPK/MMP pathways

“…Interestingly, 15 mg/L of the PTF significantly inhibited ERK activation and NCI-N1703 cell migration and invasion, but its reduction of MMP-2 and MMP-9 expression was not significant (P=0.058 and 0.061, respectively). Accordingly, we speculated that other molecular targets, such as other MMPs, may be involved in this antimetastasis mechanism that requires further investigation [29][30][31] . Taken together, the significant findings of this study include the following: the PTF may induce tumor cell apoptosis by regulating the ERK1/2 and JNK MAPK signaling pathway.…”