Tinospora crispa extract inhibits MMP-13 and migration of head and neck squamous cell carcinoma cell lines

Phienwej, Kaninut; Swasdichira, Ih-si; Amnuoypol, Surattana; Pavasant, Prasit; Sumrejkanchanakij, Piyamas

doi:10.1016/j.apjtb.2015.07.001

Cited by 13 publications

(9 citation statements)

References 24 publications

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“…Furthermore, cells were maintained in normal growth medium with or without DAPT supplementation for 24 h. At 0 and 24 h, images of the wound were captured at the same location using mark on phase contrast microscope to directly compare gap of the wound. Relative migration ratio was calculated based on previous study (38).…”

Section: Wound Healing Assaymentioning

confidence: 99%

Expression and influence of Notch signaling in oral squamous cell carcinoma

Osathanon

Nowwarote

Pavasant

2016

Journal of Oral Science

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Section: Wound Healing Assaymentioning

confidence: 99%

Expression and influence of Notch signaling in oral squamous cell carcinoma

Osathanon

Nowwarote

Pavasant

2016

Journal of Oral Science

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“…Here we observed a high MMP-13 expression in MIA induced hADMSCs in comparison to DM preconditioned hADMSCs ( Figure 5e ). Prior studies conducted also indicates downregulation of MMP-13 has been reported in human HNSCC, HN22, HSC-3 and RAW 264.7 cell lines using Tinospora crispa and Morus alba plant extracts [ 81 , 82 ].…”

Section: Discussionmentioning

confidence: 99%

Daphne mucronataenhances cell proliferation and protects human adipose stem cells against monosodium iodoacetate induced oxidative stressin vitro

et al. 2020

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Mesenchymal stem cells (MSCs) are being used to treat many diseases as they exhibit great regenerative potential. However, MSC's transplantation sometimes does not yield the maximum regenerative outcome as they are unable to survive in inflammatory conditions. Several approaches including preconditioning are used to improve the survival rate of mesenchymal stem cells. One such recently reported approach is preconditioning MSCs with plant extracts. The present study was designed to evaluate the effect of Daphne mucronata extract on stressed human adipose-derived mesenchymal stem cells (hADMSCs). Isolated hADMSCs were preconditioned with different concentrations of Daphne muconata extract and the protective, proliferative, antioxidant and anti-inflammatory effect was assessed through various assays and expression analysis of inflammatory markers regulated through NF-κB pathway. Results suggest that preconditioning hADMSCs with Daphne mucronata increased the cell viability, proliferative and protective potential of hADMSCs with a concomitant reduction in LDH, ROS and elevation in SOD activity. Moreover, both the ELISA and gene expression analysis demonstrated down regulations of inflammatory markers (IL1-β, TNF-α, p65, p50, MMP13) in Daphne mucronata preconditioned hADMSCs as compared to stress. This is the first study to report the use of MIA induced oxidative stress against hADMSC's and effect of Daphne mucronata on stressed hADMSCs. Results of these studies provided evidence that Daphne mucronata protects the hADMSCs during stress conditions by down regulating the inflammatory markers and hence increase the viability and proliferative potential of hADMSCs that is crucial for transplantation purposes.

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“…In general, T. crispa stem extracts were able to reduce the cell viability of K562 cells with different incubation period. From the previous study, it was found that T. crispa stem extract reduced the cell viability of different panel of cell lines such as MCF-7, HeLa, HN22 and HSC with the highest concentration of 100 µg/mL for MCF-7 and HeLa (Ibahim et al, 2011;Phienwej et al, 2015). These studies emphasized the potential of T. crispa stem extracts have potential cytotoxic effect on K562 cell but not reducing until 50% of the cell viability.…”

Section: Aqueous Extractmentioning

confidence: 95%

Cytotoxic Activity of Tinospora crispa Crude Extracts (Stem) Against K562 Human Leukemia Cells

Awang

Ruzali

Mohamad

et al. 2019

OnLine Journal of Biological Sciences

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Tinospora crispa (T. crispa) is traditionally used as an herbal medicine for treatment of gout and is an analgesic, anti-inflammatory and antihyperuricemic agent. Cytotoxicity of hexane, dichloromethane, ethanol and aqueous extracts of T. crispa stem against K562 cells was assessed. Crude extracts of T. crispa stem were obtained by sonication and maceration methods. T. crispa stem powder was macerated and sonicated with hexane, dichloromethane, ethanol and aqueous (1:10 w/v). Extraction via sonication method yielded higher product compared to maceration method in which the yield percentage of crude extract was 0.97%, 1.92%, 5.27% and 12.80% for hexane, dichloromethane, ethanol and aqueous respectively. The yield percentage obtained from maceration method for hexane, dichloromethane, ethanol and aqueous was 0.91%, 1.71%, 3.01% and 12.05% respectively. The cytotoxicity of the crude extracts were tested against K562 cells using 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide assay (MTT) for 24, 48 and 72 h with the highest concentration of 200 µg/mL. The MTT assay result showed that T.crispa stem crude extract significantly reduced cell viability (p<0.005) at the concentration more than 200ug/ml for 72 h treatment using hexane, dichloromethane, ethanol and aqueous extract at concentrations 200 µg/mL, while 100 µg/mL for aqueous extract only."4). The IC 50 value for dichloromethane and ethanol extracts of T. crispa was 158±1.33 µg/mL and 172±6.00 µg/mL respectively. On the other hand, 72 h treatment caused significant effects on the viability of cells treated with hexane and ethanol extracts of T. crispa. Hence, this study indicated that dichloromethane and ethanol extracts of T. crispa were able to inhibit cell proliferation based on the results from the MTT assay. Further study should be conducted to elucidate the potential of T. crispa crude extract before it can be developed as a new anti-leukemic agent.

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Tinospora crispa extract inhibits MMP-13 and migration of head and neck squamous cell carcinoma cell lines

Cited by 13 publications

References 24 publications

Expression and influence of Notch signaling in oral squamous cell carcinoma

Expression and influence of Notch signaling in oral squamous cell carcinoma

Daphne mucronataenhances cell proliferation and protects human adipose stem cells against monosodium iodoacetate induced oxidative stressin vitro

Cytotoxic Activity of Tinospora crispa Crude Extracts (Stem) Against K562 Human Leukemia Cells

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