Expression of protein p27 is associated with progression and prognosis in laryngeal cancer

Fan, Guo-Kang; Fujieda, Shigeharu; Sunaga, Hiroshi; Tsuzuki, Hideaki; Ito, Nobuhisa; Saitô, Hitoshi

doi:10.1097/00005537-199905000-00025

Cited by 32 publications

(27 citation statements)

References 17 publications

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“…The percentage of p21-and p27-positive tumor cells was expressed as a ratio of positive cells to the total number of tumor cells counted. In accordance with previous studies (12,15,16), tumors with ≤10% of p21-and p27-positive cells were considered to be negative for p21 and p27 expression, respectively.…”

Section: Immunohistological Stainingmentioning

confidence: 71%

“…With regard to correlation with apoptosis, recent immunohistological study showed a significant correlation between p27 expression and increased apoptosis in oral and oropharygeal SCC (11). With regard to correlation with clinical outcomes, some reports have indicated a significant correlation between decreased expression levels of p21 or p27 and poorer prognoses in laryngeal SCC (12), but contradictory results were reported (10). Although a significant correlation between higher proliferation activities of tumor cells and poorer prognoses was reported for head and neck SCC except for maxillary SCC (13), no correlation was reported in laryngeal SCC (14).…”

Section: Introductionmentioning

confidence: 99%

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Loss of p21 expression is associated with p53 mutations and increased cell proliferation and p27 expression is associated with apoptosis in maxillary sinus squamous cell carcinoma

Bandoh

Hayashi

Takahara

et al. 2005

Acta Oto-Laryngologica

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Section: Immunohistological Stainingmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Loss of p21 expression is associated with p53 mutations and increased cell proliferation and p27 expression is associated with apoptosis in maxillary sinus squamous cell carcinoma

Bandoh

Hayashi

Takahara

et al. 2005

Acta Oto-Laryngologica

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“…Some authors have investigated the clinico-prognostic role of p27 in LSCCs. Absent p27 expression has been statistically associated with advanced clinical stage, lymph node involvement and distant metastasis [13]. However, Korkmaz et al [32] found that p27 overexpression was a [56] failed to find any statistically significant correlation between p53 expression and disease stage but did find statistically significant associations with histological grades (I, II, and III).…”

Section: Discussionmentioning

confidence: 99%

“…The evaluation of p27 expression has been considered clinically relevant to HNSCC. In patients with oral and laryngeal cancers, reduced p27 expression has been shown to be correlated with poor prognoses, unfavorable treatment responses and metastases [13,34,50,61].…”

Section: Introductionmentioning

confidence: 99%

Possible implication of Mdm2 as a prognostic marker in invasive laryngeal carcinoma

Hassumi‐Fukasawa

Miranda-Camargo

Guimarães

et al. 2012

Eur Arch Otorhinolaryngol

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Laryngeal squamous cell carcinoma is one of the most common malignant neoplasms of the head and neck. In Brazil, laryngeal tumors represent 2% of all cancers and are associated with approximately 3,000 deaths annually. Human papillomavirus (HPV) has been reported to play an important role in the etiology of laryngeal cancer. The aim of the present study was to evaluate the expression of p53, p27, and Mdm2 in laryngeal carcinomas. Sixty-three larynx biopsies were selected for the study, including 9 in situ laryngeal carcinomas, 27 laryngeal carcinomas without metastasis and 27 laryngeal carcinomas with metastasis. Twenty-seven cervical lymph nodes from patients with metastatic lesions were also evaluated. The expression levels of p53, p27, and Mdm2 were assessed by immunohistochemistry using a computer-assisted system. HPV detection and typing were performed using PCR, and the HPV types that were evaluated included HPV 6, 11, 16, 18, 31 and 33. Out of 63 patients, 53 (84.1%) were positive for β-globin (internal control), and 10 (15.9%) were β-globin negative and therefore excluded from the evaluation. Thus, 7 (13.2%) out of 53 patients were HPV positive, and 46 (86.8%) out of 53 patients were HPV negative. Statistically significant differences (p < 0.05) in Mdm2 expression levels were observed in the in situ laryngeal carcinoma samples compared with the laryngeal carcinoma samples with metastasis. No statistically significant differences (p > 0.05) in either p53 or p27 expression levels were detected. These findings suggest that Mdm2 may be associated with the invasiveness and aggressiveness of laryngeal carcinomas.

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“…Positive staining for COX-2 was located mainly in the cytosol, and p27 in the nucleus. Immunocytochemical staining was classified as follows [10] : number of positive cells < 10% was deemed as negative (-), 10%-25% as (+), 25%-50% as (++), and > 50% as (+++). …”

Section: Methodsmentioning

confidence: 99%

Influence of paeonol on expression of COX-2 and p27 in HT-29 cells

Ye¹,

Deng²,

Zhang³

2009

WJG

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AIM:To investigate the effect of paeonol on controlling the proliferation of colorectal cancer cell line HT-29 and to discuss its possible mechanism. METHODS:The inhibitory effect of paeonol on proliferation of HT-29 cells was detected by MTT assay. The results of apoptosis were measured by flow cytometry. Immunocytochemical staining w a s p e r f o r m e d t o d e t e c t t h e e x p r e s s i o n o f cyclooxygenase-2 (COX-2) and protein p27 in HT-29 cells treated with paeonol at different concentrations. Reverse transcription-polymerase chain reaction (RT-PCR) was used for mRNA analysis. RESULTS:From the data of both MTT and flow cytometry, we observed that cell proliferation was inhibited by different concentrations of paeonol. By immunocytochemical staining, we found that HT-29 cells treated with paeonol (0.024-1.504 mmol/L) reflected reduced expression of COX-2 and increased expression of p27 in a dose-dependent manner. RT-PCR showed that paeonol down-regulated COX-2 and up-regulated p27 in a dose-and time-dependent manner in HT-29 cells. CONCLUSION:One of the apoptotic mechanisms of paeonol is down-regulation of COX-2. p27 is upregulated simultaneously and plays an important part in controlling cell proliferation and is a crucial factor in the Fas/FasL apoptosis pathway.

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Expression of protein p27 is associated with progression and prognosis in laryngeal cancer

Abstract: We concluded that assessment of p27 expression is useful as a prognostic factor for laryngeal SCC and of value in selecting patients with laryngeal SCC for aggressive therapy.

Cited by 32 publications

References 17 publications

Loss of p21 expression is associated with p53 mutations and increased cell proliferation and p27 expression is associated with apoptosis in maxillary sinus squamous cell carcinoma

Loss of p21 expression is associated with p53 mutations and increased cell proliferation and p27 expression is associated with apoptosis in maxillary sinus squamous cell carcinoma

Possible implication of Mdm2 as a prognostic marker in invasive laryngeal carcinoma

Influence of paeonol on expression of COX-2 and p27 in HT-29 cells

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