Expression of p63 Protein to Differentiate Benign Prostatic Hyperplasia and Carcinoma of Prostate in Pakistani Population

Nisar, Bushra; Sarwar, Naureen; Sharif, Sadia; Hameed, Asim; Naz, Shazia

doi:10.21649/akemu.v23i2.1576

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…13 It was observed that all 25 (100%) cases of NHP showed positive p63 expression and all 25 cases of adenocarcinoma showed negative expression of p63 as a basal cell marker. The findings of the current study were similar with findings of Nisar et al and Kalantari et al 15,16…”

Section: Figure 1: Age Distribution Of Study Population (N=50)supporting

confidence: 93%

Evaluation of mucin histochemistry in relation to p63 expression in nodular hyperplasia and adenocarcinoma of prostate

Sweety

Sultana²,

Islam³

et al. 2023

Int J Res Med Sci

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Background: Prostate cancer is a leading cause of death in men. Nodular hyperplasia and adenocarcinoma are common causes of prostatic enlargement. Diagnosis of these lesions on routine biopsies can be difficult for pathologists. Immunohistochemical stain p63 can help, but it is costly and not widely available. The present study aimed to evaluate the usefulness of mucin histochemistry in relation to p63 expression in differentiating nodular hyperplasia and adenocarcinoma of prostate. Methods: This study was conducted in the department of pathology at Dhaka medical college from July 2018 to June 2020. 50 cases of prostatic lesions (25 NHP and 25 adenocarcinoma) were examined using histomorphology. The sections were stained with periodic acid Schiff (PAS) to identify neutral mucins and Alcian blue (2.5 pH) to identify acidic mucins. Additionally, p63 antibody was used in immunohistochemistry. Results: NHP showed positivity for neutral mucin (96% with PAS stain) but not for acidic mucin (Alcian blue stain), while prostatic carcinoma showed positivity for both neutral mucin (28%) and acidic mucin (44%). The grade group 1 tumors of prostatic carcinoma showed 100% positivity for acid mucin, with a decrease in Alcian blue staining as the grade increased. P63 was positive in 100% of NHP cases and negative in 100% of prostatic carcinoma cases. Conclusions: Positivity for acidic mucins with Alcian blue stain can be a helpful diagnostic tool to differentiate well differentiated adenocarcinomas from benign lesions where facility for p63 immuno-stain is not available and poor people who cannot afford the cost of immunohistochemistry.

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Section: Figure 1: Age Distribution Of Study Population (N=50)supporting

confidence: 93%

Evaluation of mucin histochemistry in relation to p63 expression in nodular hyperplasia and adenocarcinoma of prostate

Sweety

Sultana²,

Islam³

et al. 2023

Int J Res Med Sci

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show abstract

“…No study has not been conducted on NKX3. 14,15,16 Our research ndings coincide with research carried out in Sudan. Out of the 40 cases, Immunohistochemistry revealed loss of NKX3.1 expression in 26 cases of prostatic adenocarcinoma, whereas 14 cases showed positive expression.…”

Section: Discussionsupporting

confidence: 58%

Immunohistochemical Expression of Nkx3.1 in Prostatic Adenocarcinoma and Benign Prostatic Hyperplasia at a Tertiary Care Hospital in Karachi

Hussain,

Raza,

Hussain

et al. 2023

J Gandhara Med Dent Sci

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OBJECTIVES To determine NKX3.1 expression in prostatic adenocarcinoma and benign prostate hyperplasia on Immunohistochemistry at a tertiary care hospital in Karachi. METHODOLOGY 74 prostatic specimens were recruited in this comparative cross-sectional study at the Department of Pathology, Pakistan Navy Station Shifa Hospital, Karachi, from January 2018 to February 2019. Of these, 37 specimens were of prostatic adenocarcinoma, and 37 were benign prostate hyperplasia. All specimens were subjected to immunohistochemical staining with NKX3.1. Statistical analysis was done by using SPSS version 23.0. The association of the extent of NKX3.1 staining between the adenocarcinoma and hyperplasia group was assessed using the Chi-square test x2. RESULTSOf the 37 cases of benign prostatic hyperplasia subjected to NKX3.1 staining, 32 showed positive staining with strong to moderate intensity. No staining was observed in 5 cases. 37 cases of adenocarcinoma prostate stained for NKX3.1 revealed positive staining in 30 cases with strong to moderate intensity. Negative staining was seen in 7 cases. The prostatic adenocarcinoma showed a statistically significant association of NKX3.1 positivity compared to benign prostatic hyperplasia cases. The p-value was found to be 0.03. CONCLUSION NKX3.1 staining was highly specific for prostate epithelium, as it was positive in most cases. This immune marker was useful for distinguishing prostatic origin in the context of metastatic lesions. Adding NKX3.1 protein staining to a panel of features may add value to the diagnosis if applied in the appropriate clinicopathologic context.

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Clinicopathological and immunohistochemical evaluation of lonidamine-entrapped lipid–polymer hybrid nanoparticles in treatment of benign prostatic hyperplasia: An experimental rat model

Sengel-Türk

Alçığır

Ekim

et al. 2020

European Journal of Pharmaceutics and Biopharmaceutics

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Expression of p63 Protein to Differentiate Benign Prostatic Hyperplasia and Carcinoma of Prostate in Pakistani Population

Cited by 3 publications

References 10 publications

Evaluation of mucin histochemistry in relation to p63 expression in nodular hyperplasia and adenocarcinoma of prostate

Evaluation of mucin histochemistry in relation to p63 expression in nodular hyperplasia and adenocarcinoma of prostate

Immunohistochemical Expression of Nkx3.1 in Prostatic Adenocarcinoma and Benign Prostatic Hyperplasia at a Tertiary Care Hospital in Karachi

Clinicopathological and immunohistochemical evaluation of lonidamine-entrapped lipid–polymer hybrid nanoparticles in treatment of benign prostatic hyperplasia: An experimental rat model

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