Background: Diabetes mellitus is an extremely common endocrine metabolic disorder that results in chronic hyperglycemia. It has effects on various tissues of the body. Due to this increased blood glucose levels considerable cellular changes occur in oral cavity as well. This field has attracted little research. The aim of the study was to analyze the changes in morphology and cytomorphometric measurements in the buccal mucosal cells of type 2 diabetic patients.

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Background:  Prostate cancer is the world’s foremost and second cause of cancer associated death typically in males after lung cancer. Histopathological diagnosis of prostatic hyperplasia and prostatic carcinoma can be challenging. The expression of p63 can be used in diagnosis and differentiation of benign pro-static hyperplasia from carcinoma of prostate.Materials and Methods:  We studied sixty prostatic biopsies obtained by TURP and radical prostatectomy. For each case, clinical data was collected. The tissue sections were then diagnosed on basis of routine hematoxylin and eosin. Then immunohistochemical (IHC) analysis was performed on routinely processed, forma-lin-fixed, paraffin embedded tissue.  We also analyzed P63 expression in regions of benign prostatic hyperplasia and prostatic adenocarcinoma. Moreover, detailed examination of tissue sections was observed with light microscopy.Results:  Mean age of patients with adenocarcinoma was 70 ± 12 years however mean age in prostate hyperplasia cases was 66 ± 8 years. With the use of Receiver Operative Characteristic Curve (ROC), the optimal cut point found of PSA was 30.0ng/ml. The results showed that prostate specific antigen (PSA) at this cutoff had a sensitivity of 71.4%, with specificity of 74.4% and accuracy of 73.3%. So PSA could not be considered reliable independently for the diagnosis of carcinoma of prostate. Benign cases in the present study were exclusively positive for immunohistoche-mical expression of p63 while all the cases of prostatic carcinomas expressed negative pattern of staining.Conclusion:   Immunostaining with p63 is useful to differentiate benign prostatic hyperplasia from pro-static carcinoma, so it may be used as valuable tool in the diagnosis of prostatic carcinoma.

Background: Streptococcus mutans is considered as a major microorganism causing tooth decay, affecting individuals globally. Different types of chewing sticks possess anti cariogenic properties, traditionally been consumed in maintaining dental hygiene. Usage of these sticks can be economical in developing and rural regions where dental caries is a major health concern. Objective: The current study aims to evaluate antibacterial action of different type of chewing sticks extract on Streptococcus mutans. Methods: Aqueous extracts of Azadiracta indica (Neem), Melia azedarach (Bakain), Mangifera indica (Mango), Salvadora persica (Peelu), Terminalia chebula (Harhar), Dalbeigia sissoo (Tali) and Juglans regia (Akhrot) plants which have justified folk use were prepared in Punjab University College of Pharmacy, Punjab University. Next, in vitro antimicrobial activity was studied by broth dilution method in Post Graduate Medical Institute, Lahore. Moreover, minimum inhibitory concentration (MIC) as well as the minimum bactericidal concentration (MBC) were quantitatively evaluated on basis of turbidity index. Results: There is a significant effect of aqueous extracts of plants on the bacterial inhibition. MIC and MBC values were in the range of 6.125 to 100 mg/mL against S. mutans. The notable effect occurred with aqueous extracts of Azadiracta indica showing MIC and MBC values as 6.25mg/mL and 12.5 mg/mL, respectively. Alternatively, Salvadora persica demonstrated 8.33mg/mL and 16.66 mg/mL values of these parameters Conclusions: Each plant studied exhibited moderate to high antibacterial activity against tested bacterial strain. However, Azadirachta indica and Salvadora persica aqueous extracts showed promising effect against Streptococcus mutans

To assess parents knowledge and awareness about oral health and hygiene and to investigate oral hygiene practices of their children . Materials and Methods :A questionnaire based survey was conducted among randomly selected parents of 300 children attending the outdoor Paedodontic department of Punjab dental hospital, Lahore from different cities of Punjab province. Total 300 distributed questionnaires, each having two parts were filled and returned by parents on next appointment visit of their children. The data was analyzed using SPSS software version 21.0. Ethical clearance for the study was obtained from institutional/hospital Ethical Committee. Results:74.35%  parents acknowledged the importance of oral hygiene. 35.3% parents considered good oral health important for general health. Most of the parents (64.7%) didn’t know that primary teeth affect permanent teeth and 70% of parents didn’t think that primary carious teeth require treatment. Majority of the parents (78.3% and 55.7% respectively) didn’t know that pacifiers/bottles(feeders) affect the infants /childs oral health and should be replaced by feeding cups when the child is able to hold it. 82.7% parents knew that sweet sticky food causes caries however 61.3% didn’t consider frequent intake of artically flavoured juices,beverages detrimental for oral health. Most of the parents (75%) considered tooth brushing important but 57.7% didn’t know that bedtime brushing is extremely beneficial for oral health. 62.3% parents did not know that fluoride toothpastes provide protection against dental caries and  51% of the parents didn’t consider routine dental checkups necessary. According to the data, 39.7% parents initiated teeth brushing of their children at the age of 19-24 months, followed by 25-30 months (25%). Majority of the children were supervised/ helped in cleaning teeth (92%). Most of the children were found to brush their teeth only once a day (73%). Most frequently used tooth cleaning aid was tooth brush & tooth paste (66%) followed by maswak (18% ). Most of the children spent thirty seconds on brushing (62.3%) followed by one minute (17.7%). Most of the children brush their teeth in horizontal direction (57%) followed by haphazard (34%) direction.  65.7%) of the parents didn’t remember when they last changed their child’s tooth brush. Majority of the parents (45.3%) took their child for dental visit more than one year ago and main reason for visit was pain (71%). Most of the children took sweet sticky food several times a day (65%). Conclusion:Parents awareness about oral health and hygiene is the need of hour to improve oral health of children in our country. The results of this study lay emphasis on conduction of various educational and motivational programmes among the parents about significance of oral health and  hygiene at grassroots level.