Expression of Bone Matrix Protein mRNAs by Primary and Cloned Cultures of the Regenerative Phenotype of Human Periodontal Fibroblasts

Abstract

The successful regeneration of periodontal tissues is dependent, in part, on the ability of cells to reconstitute the mineralized tissues of cementum and bone. The aim of the present study was to characterize regeneration-associated cells in terms of their ability to express mineralized tissue macromolecules. Following guided tissue regeneration, cell cultures were established from regenerating tissue, periodontal ligament, and gingiva. Additionally, these cells were transfected, and single-cell-derived clones were established. Following treatment with platelet-derived growth factor-BB and insulin-derived growth factor-1, the presence of mRNA for alkaline phosphatase, osteocalcin, bone sialoprotein, osteopontin, and bone morphogenetic proteins-2 and-4 was assessed. The three cell types expressed similar mRNA levels for alkaline phosphatase, bone morphogenetic protein-2, and bone morphogenetic protein-4, whereas the expression of osteopontin, osteocalcin, and bone sialoprotein was greater in the periodontal ligament and regenerating tissue fibroblasts compared with the gingival fibroblasts. The two growth factors did not affect the expression of any of the genes. This study has identified markers that correlate with the known ability of periodontal ligament and regenerating tissue-derived fibroblasts to facilitate regeneration of the mineralized tissues of the periodontium.

Periodontal regeneration following transplantation of proliferating tissue derived from periodontal ligament into class III furcation defects in dogs

“…None of these processes was noted in the control group. These results support the findings of our previous study showing that treatment without pPDL inhibited periodontal wound healing (12). In the present study, epithelial downgrowth occurred in the furcation defect in the control group.…”

“…This discovery was consistent with the findings reported by Ivanovski et al (12). They reported that mRNA expression of alkaline phosphatase, osteonectin, bone sialoprotein, and osteopontin increased in pPDL (12). The results of those studies suggest that periodontal regeneration is achieved through pPDL because of its abundant matrix components specific to distinct periodontal components.…”

Regulatory mechanisms of periodontal regeneration

“…They have established three cultured cell types from regenerating tissue, the periodontal ligament and gingiva following guided tissue regeneration. The three celltypes express similar mRNA levels for alkaline phosphatase, bone morphogenetic protein-2, and bone morphogenetic protein-4, whereas the expression of osteopontin, osteocalcin, and bone sialoprotein is greater in the periodontal ligament and regenerating tissue fibroblasts compared with gingival fibroblasts (Ivanovski et al, 2001a). King and Hughes (2001) examined the effects of different delivery systems upon bone morphogenetic protein-induced periodontal regeneration.…”

Establishment of immortalized clonal cells derived from periodontal ligament cells by induction of the hTERT gene

“…In addition, the transduction of HPL cells with the hTERT gene prevented cellular senescence and extended the cell life span. PDL cells appear to have osteogenic characteristics; unlike gingival fibroblasts, PDL cells produce osteoblastrelated extracellular matrix proteins and they also show higher ALP activity (26)(27)(28). In the present study, ALP activity differed between cell clones, suggesting that clones had been isolated during the differentiation stage.…”