Establishment of immortalized clonal cells derived from periodontal ligament cells by induction of the hTERT gene

Establishment of immortalized periodontal ligament progenitor cell line and its behavioural analysis on smooth and rough titanium surface

“…Our results, however, are in line with studies dealing with gingival fibroblasts and chondrocytes, cell types exhibiting also superb morphology and proliferation on smoother titanium (Oates et al, 2005). Additionally, in a recent publication by Hacking et al, 2008 several cell types -MC3T3, TF274 (MSC cell line), murine femoral stromal cells and canine bone marrow cells were investigated and cell type-specific differences were observed. For example, the MC3T3, TF274 and murine femoral stromal cells, in contrast to the canine-derived cells, proliferated and osteogenically differentiated better on plastic and polished titanium than on irregularly textured titanium.…”

“…Due to their regenerative potential but limited life span in vitro, PDL-derived cells have been isolated and immortalized independently by several groups ( Fujita et al, 2005;Pi et al, 2007;Fujii et al, 2008;. Our group has recently described the use of a lentiviral gene transfer of human telomerase reverse transcriptase (hTERT) in human MSC.…”

Establishment of cell lines that exhibit pluripotency from miniature swine periodontal ligaments

“…These immotalized PDL cell lines expressed type I collagen, ALP, and periostin. Previous studies have shown that ALP activity increased and mineralizing nodule were produced by stimulation of bone morphogenetic protein-2 [18] or combination of ascorbic acid and β-glycerophosphate [27], indicating that the induction of osteoblastic phenotype were characterized in PDL fibroblasts. However, phenotype of vascular endothelial cells such as CD31, VE-cadherin and vWF expression has not been indicated in these PDL immortalized cell lines.…”

“…Human PDL cell lines were also succeeded in established by SV40T-antigen gene transduction [25,26]. Recently, human PDL fibroblasts were immmotalized by hTERT gene transduction [27] or combination of SV40T-antigen and hTERT gene transduction [28]. These immotalized PDL cell lines expressed type I collagen, ALP, and periostin.…”

Periodontal Ligament Stem Cells

“…Several genes, such as SV 40 large T-antigen, human telomerase transcriptase, human papillomavirus 16-related E6E7, Bmi-1 and BMP4, have been transduced into mammalian PDL cells. As a consequence, clonal PDL cell lines derived from mice (Saito et al, 2002), swine (Ibi et al, 2007) and humans (Kamata et al, 2004;Fujita et al, 2005;Pi et al, 2007;Mi et al, 2011) have been developed. In addition, clonal cell lines from mouse dental follicle (DF) cells as a source of PDLSCs have also been reported (Saito et al, 2005;Luan et al, 2006).…”