Establishment of immortalized periodontal ligament progenitor cell line and its behavioural analysis on smooth and rough titanium surface

Abstract

Periodontal ligament (PDL) can be obtained from patients undergoing orthodontic treatment. PDL contains progenitor cells that can be expanded and differentiated towards several mesenchymal lineages in vitro. Furthermore, PDL-derived cells have been shown to generate bone-and PDL-like structures in vivo. Thus, PDL cells, combined with suitable biomaterials, represent a promising tool for periodontitisrelated research and PDL engineering.Here, a new PDL cell line using lentiviral gene transfer of human telomerase reverse transcriptase (hTERT) was created. HTERT-expressing PDL cells showed similar morphology and population doubling time but an extended lifespan compared to the primary cells. In addition, PDLhTERT cells expressed several characteristic genes and upon osteogenic stimulation produced a calcified matrix in vitro. When cultivated on two topographically different titanium scaffolds (MA and SLA), PDL-hTERT cells exhibited augmented spreading, survival and differentiation on smooth (MA) compared to rough (SLA) surfaces. These findings differ from previously reported osteoblast behaviour, but they are in agreement with the behaviour of chondrocytes and gingival fibroblasts, suggesting a very cell type-specific response to different surface textures.In summary, we report the testing of titanium biomaterials using a new PDL-hTERT cell line and propose this cell line as a useful model system for periodontitis research and development of novel strategies for PDL engineering.

Periodontal ligament cells as alternative source for cell-based therapy of tendon injuries: in vivo study of full-size Achilles tendon defect in a rat model

“…In the study two standard cell lines characterised before, namely PDL-(hTERT) cell line from Docheva et al (2010) and human AT-(hTERT) cell line derivative from Kohler et al (2013) were implemented. Both cell lines were cultured in DMEM/Ham′s F-12 (1:1 mixture) medium supplemented with stable glutamine (365.3 mg/L), 1 × MEM amino acids (all from Biochrom, Berlin, Germany), 10 % FBS and 1 % L-ascorbic acid-2-phosphate (both from SigmaAldrich, Steinheim, Germany) in a humidified incubator at constant 37 °C and 5 % CO 2 .…”

“…Periodontal ligament (PDL)-derived cells are of particular importance as in comparison to tendon cells, in a way that they are much more easily accessible through the oral cavity and can be simply harvested especially from extracted teeth when patients have orthodontic treatments (Miura et al, 2003;Seo et al, 2004). Recently, several groups, including our, reported that PDL contains endogenous stem/progenitor cells (Bluteau et al, 2008;Docheva et al, 2010;Ivanovski et al, 2006), which express tendon-specific genes such as scleraxis and tenomodulin (Docheva et al, 2010).…”

Investigation Of Chromium-Cobalt Coated Scaffolds On Cell Cultures In Vitro

“…24 It has been proven that the surface of metal implants plays a crucial role for the cell behavior and survival, the expression of specifc molecules and cell differentiation. 21 The complex reparative cells -biocompatible scaffolds is one the most attractive approaches for tissue regeneration. 25 …”

“…The procedures for cell culturing and storing were performed according to. 21 BioCompatiBility teSting Samples were placed in 8-well glass slides with a lid for cell cultures. 6 x 10 4 cells were added to each well and incubated for 96 hours.…”

Periodontal ligament cell behavior on different titanium surfaces

“…The behavior of cellular population in contact with a metal depends directly on the microscopic properties of implants, such as topography, chemicals and surface energy [4,5]. While the effects of different types of surface treatments of titanium have been well-defined in osteoblastic cells and mesenchymal stem/stromal cells [13][14][15][16][17][18][19][20], studies focused on periodontal ligament (PDL) cells-titanium surface interactions are limited [21][22][23][24].…”