Periodontal Ligament Stem Cells

Maeda, Hidefumi; Wada, Naohisa; Fujii, Shinsuke; Tomokiyo, Atsushi; Akamine, Akifumi

doi:10.5772/23749

Cited by 9 publications

(6 citation statements)

References 113 publications

(130 reference statements)

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“…Previous studies have shown that PDL tissue contains undifferentiated cells that can differentiate into PDL fibroblasts, osteoblasts and cementoblasts (Maeda et al. ), and thus these undifferentiated cells have crucial roles in regenerating PDL tissue (Seo et al. , Fujii et al.…”

Section: Introductionmentioning

confidence: 99%

Expression and effects of glial cell line‐derived neurotrophic factor on periodontal ligament cells

Yamamoto

Maeda

Tomokiyo

et al. 2012

J Clinic Periodontology

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Section: Introductionmentioning

confidence: 99%

Expression and effects of glial cell line‐derived neurotrophic factor on periodontal ligament cells

Yamamoto

Maeda

Tomokiyo

et al. 2012

J Clinic Periodontology

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“…16 Dental pulp (DP) forms dentin, whereas PDL is tooth-supportive connective tissue that ensures gently tooth anchorage to the alveolar bone, both providing tooth nutrition, protection and sensory perception, together contributing to the tooth longevity. [17][18][19][20] Since PDL and DP are soft, connective tissues surrounded by hard, mineralized tissues, regulation of mineralization level is the main physiological demand within these tissues in order to adapt functional changes. 18,21,22 While DPSCs contribute to replacement of damaged tissue and repair of complete tooth, PDLSCs have predominant role in tooth functions and development.…”

mentioning

confidence: 99%

IL‐33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells

et al. 2018

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Objectives Soluble IL‐33 (interleukin (IL)‐1‐like cytokine) acts as endogenous alarm signal (alarmin). Since alarmins, besides activating immune system, act to restore tissue homeostasis, we investigated whether IL‐33 exerts beneficial effects on oral stem cell pull. Materials and Methods Clonogenicity, proliferation, differentiation and senescence of stem cells derived from human periodontal ligament (PDLSCs) and dental pulp (DPSCs) were determined after in vitro exposure to IL‐33. Cellular changes were detected by flow cytometry, Western blot, immunocytochemistry and semiquantitative RT‐PCR. Results IL‐33 stimulated proliferation, clonogenicity and expression of pluripotency markers, OCT‐4, SOX‐2 and NANOG, but it inhibited ALP activity and mineralization in both PDLSCs and DPSCs. Higher Ki67 expression and reduced β‐galactosidase activity in IL‐33‐treated cells were demonstrated, whereas these trends were more conspicuous in osteogenic medium. However, after 7‐day IL‐33 pretreatment, differentiation capacity of IL‐33‐pretreated cells was retained, and increased ALP activity was observed in both cell types. Results showed that IL‐33 regulates NF‐κB and β‐catenin signalling, indicating the association of these molecules with changes observed in IL‐33‐treated PDLSCs and DPSCs, particularly their proliferation, pluripotency‐associated marker expression and osteogenesis. Conclusions IL‐33 treatment impairs osteogenesis of PDLSCs and DPSCs, while increases their clonogenicity, proliferation and pluripotency marker expression. After exposure to IL‐33, osteogenic capacity of cells stayed intact. NF‐κB and β‐catenin are implicated in the effects achieved by IL‐33 in PDLSCs and DPSCs.

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“…The loss of PDL due to inflammation, injury or aging leads to ankylosis, which causes many clinical problems, such as root resorption. Although the PDL is composed of heterogeneous cell types such as fibroblasts, osteoblasts, osteoclasts, cementoblasts, endothelial cells, sensory cells and their progenitor or stem cells (Beertsen et al, 1997;Maeda et al, 2011;Ueda-Maeda, 2006), fibroblasts are responsible for the fibrous attachment of the PDL. The components of PDL include type I collagen (70-80%), other types of collagen (type III, IV, V, VI and XII collagens), and other extracellular matrix proteins such as periostin (Postn), glycoproteins and proteoglycans, all of which are mainly produced by PDL fibroblasts (Aukkarasongsup et al, 2013;Beertsen et al, 1997;Ma et al, 2011;Rios et al, 2005;Sloan, 1979;Ahuja, 2012).…”

Section: Introductionmentioning

confidence: 99%

Mohawk transcription factor regulates homeostasis of the periodontal ligament

et al. 2016

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The periodontal ligament (PDL), which connects the teeth to the alveolar bone, is essential for periodontal tissue homeostasis. Although the significance of the PDL is recognized, molecular mechanisms underlying PDL function are not well known. We report that mohawk homeobox (Mkx), a tendon-specific transcription factor, regulates PDL homeostasis by preventing its degeneration. Mkx is expressed in the mouse PDL at the age of 10 weeks and expression remained at similar levels at 12 months. In Mkx −/− mice, agedependent expansion of the PDL at the maxillary first molar (M1) furcation area was observed. Transmission electron microscopy (TEM) revealed that Mkx −/− mice presented collagen fibril degeneration in PDL with age, while the collagen fibril diameter gradually increased in Mkx +/+ mice. PDL cells lost their shape in Mkx −/− mice, suggesting changes in PDL properties. Microarray and quantitative polymerase chain reaction (qPCR) analyses of Mkx −/− PDL revealed an increase in osteogenic gene expression and no change in PDL-and inflammatory-related gene expression. Additionally, COL1A1 and COL1A2 were upregulated in Mkxoverexpressing human PDL fibroblasts, whereas osteogenic genes were downregulated. Our results indicate that Mkx prevents PDL degeneration by regulating osteogenesis.

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Periodontal Ligament Stem Cells

Cited by 9 publications

References 113 publications

Expression and effects of glial cell line‐derived neurotrophic factor on periodontal ligament cells

Expression and effects of glial cell line‐derived neurotrophic factor on periodontal ligament cells

IL‐33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells

Mohawk transcription factor regulates homeostasis of the periodontal ligament

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