Periodontal regeneration following transplantation of proliferating tissue derived from periodontal ligament into class III furcation defects in dogs

Abstract

The aim of this study was to evaluate the healing of class III furcation defects following

Autologous periodontal ligament cells in the treatment of class II furcation defects: a study in dogs

“…These findings are in accordance with previous studies, which demonstrated the potential of in situ tissue engineering using PDL cells for the periodontal regeneration (Nakahara et al 2004, Akizuki et al 2005, Murano et al 2006, Gault et al 2010). Nakahara et al (2004), evaluating the effect of PDL cells seeded on periodontal fenestration defects, demonstrated that these cells induced cementum regeneration on the root surface.…”

“…Fenestration and dehiscence defects, used in previous studies, have thin buccal alveolar plate and promote minimal space between the flap and the root surface contributing for the lack of effect in the bone regeneration. On the other hand, similar positive bone response was reported by Murano et al (2006) that applied PDL cells in class III furcation in dogs and observed an accelerated bone formation (84.8%) when compared with the control group (12.2%).…”

“…Recently, human periodontal ligament stem cells were isolated and characterized as a population of multipotent stem cells capable of forming cementum and periodontal ligament tissues upon in vivo transplantation (Seo et al 2004). Animal studies have suggested that periodontal ligament cells (PDL cells), when cultured in scaffolds and then transplanted into periodontal defects, have multiple differentiation properties to regenerate periodontal tissues (Nakahara et al 2004, Akizuki et al 2005, Murano et al 2006, Gault et al 2010.…”

Periodontal ligament‐derived cells for periodontal regeneration in animal models: a systematic review

“… reported a study involving 15 mixed‐breed dogs, in which the use of tissue derived from periodontal ligament to regenerate Class III furcation defects was evaluated, and identified that implantation of tissue containing periodontal ligament‐derived cells significantly increased the extent of periodontal tissue and cementum regeneration covering the circumference of the defect in dogs that received periodontal ligament tissue compared with controls. Additionally, implantation of periodontal ligament tissue increased the amount of new bone formation, with 84.8 ± 3.9% of the defect height being filled with new bone in the periodontal ligament tissue group compared with 12.2 ± 1.3% ( p < 0.01) in control animals . The periodontal ligament‐derived cells used in these experiments were obtained using a similar protocol to that used by Dogan et al .…”

bFGF‐loaded HA‐chitosan: A promising scaffold for periodontal tissue engineering

“…Tissue engineering principles may provide new approaches to overcome the obstacles encountered during the attempts to regenerate the periodontal tissues. Tissue engineering applications to engineer the periodontal tissues using PDL cells, cementoblasts, bone marrow stromal cells, and periosteal cells have provided promising result in animal models 5–9…”