Expression of a myeloid marker on TdT-positive acute lymphocytic leukemic cells: evidence by double-fluorescence staining

Bettelheim, Peter; Paietta, Elisabeth; Majdic, Otto; Gadner, Helmut; Schwarzmeier, J.D.; Knapp, Walter

doi:10.1182/blood.v60.6.1392.bloodjournal6061392

Cited by 49 publications

(8 citation statements)

References 24 publications

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“…Pui has demonstrated the presence of CALLA in 3 cases of peroxidase positive acute leukaemia (23). Alternatively, the expression of a myeloid marker has been demonstrated in 2 cases of ALL (24). In these rare cases, the coexistence of lymphoid and myeloid markers on the same cells could be explained by the existence of a common progenitor cell which could retain both phenotypes during leukaemic transformation.…”

Section: Discussionmentioning

confidence: 99%

Expression of a B‐lymphoid differentiation antigen (CD 19) on acute non‐lymphoblastic leukaemia cells

Campos

Guyotat

Gentilhomme

et al. 1987

European J of Haematology

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The expression of CD 19 (B4) surface antigen was investigated in 41 cases of acute myeloid leukaemia (AML), and 8 cases of acute undifferentiated leukaemia (AUL).Diagnosis was carried out by usual cytological and cytochemical means, and leukaemias were classified according to the FAB classification. CD 19 antigen is thought to be expressed on B lymphoid cells only. However, 2 cases out of 9 FAB M1, M2 and M3 subtypes were weakly stained (20 to 25%) by B4 antibody; 7 out of 11, and 17 out of 18 cases of M4 and M5 subtypes were markedly stained (25 to 70% of cells). 3 cases of AUL were also stained. Moreover, B4 expression was often correlated with expression of monocyte-associated antigens, as recognized by CRIS6, My9, KiMl and UCHMl antibodies. It is concluded that B4 antigen is probably expressed before B differentiation and is retained in early stages of monocytic differentiation.

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Section: Discussionmentioning

confidence: 99%

Expression of a B‐lymphoid differentiation antigen (CD 19) on acute non‐lymphoblastic leukaemia cells

Campos

Guyotat

Gentilhomme

et al. 1987

European J of Haematology

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“…It should be noted that Bettelheim and co-workers have also described two cases of acute leukaemia in which overlapping staining for TdT and a granulocyte-associated antigen, detected by Mab VIM-D5, was observed [29]. However, both appeared to be cases of childhood ALL.…”

Section: Discussionmentioning

confidence: 60%

Coexpression of p165 myeloid surface antigen and terminal deoxynucleotidyl transferase: A comparison of acute myeloid leukaemia and normal bone marrow cells

et al. 1986

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A double immunofluorescence technique, using antibodies to terminal transferase (TdT) and a 165-kilodalton myeloid differentiation antigen (p165), has been used to investigate the phenomenon of TdT expression in cases of acute myeloid leukaemia (AML). Five cases of AML were shown to have significant (18-90%) numbers of leukaemic cells that concurrently expressed both TdT and p165 myeloid surface antigen. Examination of nonleukaemic bone marrow cells showed that the vast majority of normal TdT+ cells are p165 negative. However, in 5 of the 11 samples analyzed, rare cells staining for both p165 and TdT were found. These results suggest that some cases of TdT+ AML may arise from the clonal expansion of rare "biphenotypic" precursor cells existing in normal bone marrow.

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“…The finding that colony cells of patient 4 expressed the common thymocyte related antigen T6 was unexpected. Earlier, the expression of myeloid markers on acute lymphoblastic leukaemia cells has been reported (Bettelheim et al, 1982). It was suggested that this phenomenon may reflect derepression of gene activity.…”

Section: Discussionmentioning

confidence: 97%

Variable differentiation of human acute myeloid leukaemia during colony formation in vitro: a membrane marker analysis with monoclonal antibodies

Touw

Löwenberg

1985

Br J Haematol

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The capacities of human acute myeloid leukaemia (AML) cells for differentiation were evaluated by the degree of maturation of cells following in vitro colony formation. For this purpose highly purified blast cells were seeded into culture. Exogenous differentiation inducers, other than the colony stimulating materials, were not added to the cultures. Phenotypes of the colony cells were determined by use of monoclonal antibodies directed against a number of maturation antigens. In all 10 cases marked changes in the surface phenotypes of the cells post culture were seen. Differentiation was incomplete and quite variable as compared with normal myeloid colonies. Following colony formation myeloid maturation antigens became apparent on the cells of the one patient with morphologically undifferentiated leukaemia. The residual differentiation capacities among individual cases of human AML, as revealed in vitro, are diverse and do not parallel the morphological maturation features of the blasts prior to culture according to the FAB nomenclature. This approach may be utilized to improve the classification of human AML and to disclose the lineage relationship of certain cytologically unclassifiable leukaemias.

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Expression of a myeloid marker on TdT-positive acute lymphocytic leukemic cells: evidence by double-fluorescence staining

Cited by 49 publications

References 24 publications

Expression of a B‐lymphoid differentiation antigen (CD 19) on acute non‐lymphoblastic leukaemia cells

Expression of a B‐lymphoid differentiation antigen (CD 19) on acute non‐lymphoblastic leukaemia cells

Coexpression of p165 myeloid surface antigen and terminal deoxynucleotidyl transferase: A comparison of acute myeloid leukaemia and normal bone marrow cells

Variable differentiation of human acute myeloid leukaemia during colony formation in vitro: a membrane marker analysis with monoclonal antibodies

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