Expression of a B‐lymphoid differentiation antigen (CD 19) on acute non‐lymphoblastic leukaemia cells

Campos, Lydia; Guyotat, Denis; Gentilhomme, O; Treille, Daniele; Fière, D; Germain, D

doi:10.1111/j.1600-0609.1987.tb01167.x

Cited by 21 publications

(1 citation statement)

References 21 publications

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“…Moreover in normal myeloid cells we were not able to identify CD20 by the IE method. Although other B-lineage antigens, as recently shown for CD19 (53), may be demonstrated in AML, we agree with most other authors (6,9,15) that CD20 is usually not expressed in neoplastic and normal myeloid precursors.…”

Section: Reactivity Of the Anti-cdzo Mcab Bi And If5 With Aml Cells Isupporting

confidence: 92%

Immunological typing of acute leukemias: Immunoenzymatic staining of fixed cells compared with immunofluorescence staining of unfixed cells in suspension

Ly¹,

Beiske

Larsen³

1988

European J of Haematology

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A panel of 14 monoclonal antibodies (McAb) against hematopoietic cell surface antigens was applied on mononuclear blood or bone marrow cells from 40 cases of acute leukemia in order to compare immunoenzymatic staining (IE) (alkaline phosphatase) of fixed cells with immunofluorescence staining (IF) of unfixed suspended cells. According to the immunological results, 25 cases were phenotyped as ALL and 15 cases as AML. Cases with blast crisis secondary to chronic myelogeneous leukemia (CML‐BC) were not represented in this series. In all ALL cases the two methods gave an identical antigenic distribution. In 20 our of 21 cases of non‐T‐cell ALL, a B‐cell progenitor origin was demonstrated by a positive staining reaction with the anti‐CD 19 McAb AB1 or HD37, and in 10 cases additionally with the anti‐CD20 McAb B1 or 1F5. In contrast to the results obtained with IF, IE revealed a poor preservation of the AB1 epitope on CD19, whereas the HD37 epitope was equally well demonstrated by both methods. In 15 cases of AML the distribution of positive versus negative cells with IE or IF was identical for all McAb except J5 (anti‐CALLA) (CD10) and Bl (CD20). Thus, 10/15 AML cases expressed CALLA with IE compared to 2/15 with IF. The corresponding figures for Bl were 5/15 and 0/15, respectively. Accordingly, normal myeloid precursor cells were CALLA‐positive with IE but negative with IF. The discrepancy probably reflects the fact that, whereas both intracytoplasmatic and membrane‐bound antigens are exposed in IE, only the latter are in IF. If the alteration of antigenic accessibility after fixation is considered, IE can safely be used for immunophenotyping of acute leukemia.

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Section: Reactivity Of the Anti-cdzo Mcab Bi And If5 With Aml Cells Isupporting

confidence: 92%

Immunological typing of acute leukemias: Immunoenzymatic staining of fixed cells compared with immunofluorescence staining of unfixed cells in suspension

Ly¹,

Beiske

Larsen³

1988

European J of Haematology

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Immunophenotyping in the classification of acute leukemia in adults. Interpretation of multiple lineage reactivity

Kaplan

Penchansky

Štolc

et al. 1989

Cancer

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Fifty-nine adult patients with acute leukemia were classified using a combination of the French-American-British (FAB) criteria and characterization by immunophenotyping using flow cytometric study. The authors identified 51 patients with acute myeloblastic leukemia and eight with acute lymphoblastic leukemia. This procedure permitted lineage assignment in leukemias that otherwise might have been unclassifiable. In addition, the authors demonstrated that the leukemic blasts of 29% of patients with myeloblastic disease exhibited one or more T-cell antigens on their surface. The use of immunophenotyping has greatly enhanced the authors' ability to correctly identify the lineage of acute leukemias. The data, however, must be interpreted with caution with respect to diagnosing acute mixed lineage leukemias and must be integrated with the morphologic and cytochemical evaluation of traditional classification schemes. The possible significance of T-cell markers in myeloblastic leukemia is discussed. Cancer 63:1520-1527, 1989. CUTE LEUKEMIAS are classified according to their A stem cell of origin and to a subset within that cell lineage. This assignment is based upon morphologic and cytochemical characteristics according to the French-American-British (FAB) classification system. ' This system of standardization has been useful because it has allowed for more reliable comparison of data generated from different centers and because the natural course and the response to treatment of the leukemia may be predicted on the basis of lineage and, to a lesser extent, on the basis of subtype.'" The development and commercial availability of monoclonal antibodies to surface antigens on leukemic cells, together with advances in flow cytometric technology , have dramatically improved our ability to characterize leukemic cells with respect to cell lineage.5-" The widespread use of selected monoclonal antibodies (leu

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Immunodiagnosis of acute leukemia displaying ectopic antigens: Proposal for a classification of promiscuous phenotypes

Vecchio¹,

Schiavone

Ferrara³

et al. 1989

American J Hematol

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The nature of the blast cells in 163 cases of acute leukemia was investigated by immunophenotyping, with particular emphasis on the expression of "ectopic" surface membrane structures. Although no antigen included in our panel except CD3 revealed absolute lineage restriction, immunological typing allowed a definite characterization of blast cells in more than 90% of cases. Four groups of patients were identified (A, B, C, D) with different degrees of antigen ectopic expression. We classified as group A leukemias (74%) those expressing conventional antigenic patterns, in absence of cross-lineage markers. Samples classified as group B (18%) showed a single ectopic membrane specificity, apparently discordant with the overall composite phenotype; such a "low-grade deviation" did not prevent a definite immunodiagnosis. Pattern C specimens (5%) revealed a promiscuous coexpression of markers related to different lineages (biphenotypic leukemias), whereas group D included unclassifiable phenotypes, characterized by no antigen or DR-only expression. Our findings suggest the possibility of interpreting complex phenotypic constellations of membrane markers in a consistent and logical manner.

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Expression of a B‐lymphoid differentiation antigen (CD 19) on acute non‐lymphoblastic leukaemia cells

Cited by 21 publications

References 21 publications

Immunological typing of acute leukemias: Immunoenzymatic staining of fixed cells compared with immunofluorescence staining of unfixed cells in suspension

Immunological typing of acute leukemias: Immunoenzymatic staining of fixed cells compared with immunofluorescence staining of unfixed cells in suspension

Immunophenotyping in the classification of acute leukemia in adults. Interpretation of multiple lineage reactivity

Immunodiagnosis of acute leukemia displaying ectopic antigens: Proposal for a classification of promiscuous phenotypes

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