Expression and Association of SCD Gene Polymorphisms and Fatty Acid Compositions in Chicken Cross

Furqon, Ahmad; Gunawan, Asep; Ulupi, Niken; Suryati, Tuti; Sumantri, Cece

doi:10.5398/medpet.2017.40.3.151

Cited by 8 publications

(7 citation statements)

References 22 publications

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“…HSD17B7 had been shown in multiple chicken breeds to code for one of the factors controlling fat deposition and muscle growth rate [ 43 , 44 , 45 ]. Acyl-CoA synthetase bubblegum family member 2 ( ACSBG2 ), stearoyl-CoA desaturase ( SCD ), and insulin induced gene 1 ( INSIG1 ) were other DEGs identified in multiple heat-control contrasts within the muscle, and all three genes had been previously identified in various chicken tissues as having transcriptional changes in response to changes in energy metabolism, particularly the metabolism of fatty acids [ 44 , 46 , 47 ]. The muscle DEGs identified are in agreement with changes in glycogen and energy utilization that are known to occur in muscles in response to heat stress [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Response of Liver and Muscle in Heat-Stressed Laying Hens

Wang

Jia

Hsieh

et al. 2021

Genes

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Exposure to high ambient temperature has detrimental effects on poultry welfare and production. Although changes in gene expression due to heat exposure have been well described for broiler chickens, knowledge of the effects of heat on laying hens is still relatively limited. In this study, we profiled the transcriptome for pectoralis major muscle (n = 24) and liver (n = 24), during a 4-week cyclic heating experiment performed on layers in the early phase of egg production. Both heat-control and time-based contrasts were analyzed to determine differentially expressed genes (DEGs). Heat exposure induced different changes in gene expression for the two tissues, and we also observed changes in gene expression over time in the control animals suggesting that metabolic changes occurred during the transition from onset of lay to peak egg production. A total of 73 DEGs in liver were shared between the 3 h heat-control contrast, and the 4-week versus 3 h time contrast in the control group, suggesting a core set of genes that is responsible for maintenance of metabolic homeostasis regardless of the physiologic stressor (heat or commencing egg production). The identified DEGs improve our understanding of the layer’s response to stressors and may serve as targets for genetic selection in the future to improve resilience.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Response of Liver and Muscle in Heat-Stressed Laying Hens

Wang

Jia

Hsieh

et al. 2021

Genes

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“…Genetic groups of ruminants have shown significant differences in the activity of the SCD gene product (Ivanović et al, 2016). In different organisms, there are five variants of SCD genes that are known as, SCD1-SCD5, placed at several chromosomal positions (Furqon et al, 2017). Furthermore, the expression of SCD in the muscle tissues can be affected by nutrition composition, especially n-3 PUFA contents.…”

Section: Stearoyl-coa Desaturases Effects On Human Healthmentioning

confidence: 99%

The Effects of Grass-Based versus Grain-Based Feeding of Ruminants on the Human Hygienic Status, a Review.

Al‐Shuhaib¹,

Al-Shuhaib²

2019

jwpr

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Ruminant meat quality is one of the important factors contributing to the recent spreading of several diseases, such as obesity, cancer, and cardiovascular problems, which have increased predominately. Feeding regiment plays an important role in the determination of the composition of fatty acids and meat quality in ruminants. This review aims to highlight the main factors that lie behind the variability of ruminant meat quality and its effect on human being's health. The reduction in grass-feeding decreases saliva levels in the ruminants, which has several consequences on the rumen, including a reduction in pH level, along with a reduction in the microorganism activities and conjugated linoleic acid levels. In adipose tissues, the expression of the stearoyl-CoA desaturases gene is negatively affected by the decreased conjugated linoleic acid levels in the rumen, which leads to a decreased transformation of saturated fatty acids to monounsaturated fatty acids. Therefore, the lower monounsaturated fatty acids and the parallel increase in the proportion of saturated fatty acids in the consumed meat can be associated with some human diseases. Thus, the present study provided a molecular explanation for the superiority of grass-based feeding in ruminants raised at pasture in term of production of meat with a healthier quality for consumers than those raised on grains.

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“…Several SNP have been described recently by our group by comparing breast muscle (BM) with divergent unsaturated fatty acids (Gunawan et al, 2017). Among them, a SNP of SLC23A3 c.22385690A>C gene was selected for association study.…”

Section: Dna Isolation Pcr Amplification and Snp Detectionmentioning

confidence: 99%

“…Maharani et al (2013) revealed that FASN and SCD gene association with fatty acid traits in broilers. Furthermore, Furqon et al (2017) reported that gen expression and single nucleotide polymorphisms (SNP) detection of SCD gene were significantly different on polyunsaturatd fatty acids in Indonesian kampung-broiler crossbred chickens. Investigation of novel SNP within genes cooperating with fatty acid compositions and their genomic analysis throught association analysis and mRNA expression is represent functional analysis generally applied tools to identify key important genes.…”

Section: Introductionmentioning

confidence: 99%

Preliminary study of solute carrier family 23 member 3 (SLC23A3) gene as candidate marker for fatty acid traits in Kampung-Broiler crossbred chickens

Gunawan¹,

Basril²,

Listyarini³

et al. 2018

J. Indonesian Trop. Anim. Agric.

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ABSTRAK SLC23A3 merupakan gen yang memiliki peranan penting yang mengontrol komposisi asam lemak pada daging. Tujuan dari penelitian ini adalah untuk mengidentifikasi asosiasi dan mengukur ekspresi mRNA gen SLC23A3 sebagai kandidat gen untuk komposisi asam lemak. Sebanyak 62 ekor ayam persilangan F2 Kampung × Broiler digunakan dalam penelitian ini. Komposisi asam lemak diukur pada ayam umur 12 minggu dari sampel otot paha ayam sebagai parameter penelitian. Metode analisis yang digunakan adalah PCR-RFLP dan qRT-PCR masing-masing untuk menentukan keterkaitan antara gen SLC23A3 dengan komposisi asam lemak dan mengukur tingkat ekspresi gen SLC23A3 pada fenotipe ayam dengan perbedaan komposisi asam lemak. Hasil penelitian menunjukkan SNP gen SLC23A3 pada daerah exon c.22385690 A>C berasosiasi secara nyata (P<0.05) dengan komposisi asam lemak diantaranya asam stearat (C18:0), asam elaidat (C18:1n9t) dan asam linoleat (C18:2n6c). Ekspresi gen SLC23A3 dideteksi terekspresi tinggi di hati baik pada sampel dengan kandungan asam lemak tinggi (HFA) maupun kandungan asam lemak rendah (LFA). Namun, ekspresi gen SLC23A3 tidak ditemukan adanya perbedaan pada HFA dan LFA. Hasil ini memberikan pemahaman lebih baik dan komprehensif mengenai peranan penting gen SLC23A3 terhadap komposisi asam lemak pada hati dan lebih menerangkan secara jelas kandidat seleksi berbasis genomik pada ayam terkait komposisi asam lemak.Kata kunci: keragaman, ekspresi mRNA, SLC23A3, seleksi, asam lemak tak jenuh ABSTRACT SLC23A3 is one of the key genes which control the properties of the of fatty acids content in the meat. The aim of this study was to identify the association of SNP and mRNA expression of SLC23A3 as a candidate marker for fatty acid traits (FAs). Sixty two F2 Kampung × Broilers crossbred chickens population were used in this study. FA composition was measured at 12 weeks of age from thigh meat of crossbred chicken. The PCR-RFLP and qRT-PCR were used for association study and expression analysis of SLC23A3 gene with divergent fatty acid composition. A SNP in coding region c.22385690 A>C of the SLC23A3 gene was associated (P<0.05) with fatty acid composition including stearic acid (C18:0), elaidic acid (C18:1n9t), and linoleic acid (C18:2n6c). The SLC23A3 was detected in liver from SLC23A3 as Candidate Marker for Fatty Acid Traits in Crossbred Chickens (A. Gunawan et al.) 201 high fatty acids (HFA) and low fatty acid (LFA) composition. However, gene expression of SLC23A3 were not differentially expressed between HFA and LFA. These results will explain better understanding of the key important role of the SLC23A3 in fatty acid traits within the liver and will propose SLC23A3 as a potential genomic selection for selection of chickens with fatty acid composition.

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Expression and Association of SCD Gene Polymorphisms and Fatty Acid Compositions in Chicken Cross

Cited by 8 publications

References 22 publications

Transcriptome Response of Liver and Muscle in Heat-Stressed Laying Hens

Transcriptome Response of Liver and Muscle in Heat-Stressed Laying Hens

The Effects of Grass-Based versus Grain-Based Feeding of Ruminants on the Human Hygienic Status, a Review.

Preliminary study of solute carrier family 23 member 3 (SLC23A3) gene as candidate marker for fatty acid traits in Kampung-Broiler crossbred chickens

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