ABSTRAK SLC23A3 merupakan gen yang memiliki peranan penting yang mengontrol komposisi asam lemak pada daging. Tujuan dari penelitian ini adalah untuk mengidentifikasi asosiasi dan mengukur ekspresi mRNA gen SLC23A3 sebagai kandidat gen untuk komposisi asam lemak. Sebanyak 62 ekor ayam persilangan F2 Kampung × Broiler digunakan dalam penelitian ini. Komposisi asam lemak diukur pada ayam umur 12 minggu dari sampel otot paha ayam sebagai parameter penelitian. Metode analisis yang digunakan adalah PCR-RFLP dan qRT-PCR masing-masing untuk menentukan keterkaitan antara gen SLC23A3 dengan komposisi asam lemak dan mengukur tingkat ekspresi gen SLC23A3 pada fenotipe ayam dengan perbedaan komposisi asam lemak. Hasil penelitian menunjukkan SNP gen SLC23A3 pada daerah exon c.22385690 A>C berasosiasi secara nyata (P<0.05) dengan komposisi asam lemak diantaranya asam stearat (C18:0), asam elaidat (C18:1n9t) dan asam linoleat (C18:2n6c). Ekspresi gen SLC23A3 dideteksi terekspresi tinggi di hati baik pada sampel dengan kandungan asam lemak tinggi (HFA) maupun kandungan asam lemak rendah (LFA). Namun, ekspresi gen SLC23A3 tidak ditemukan adanya perbedaan pada HFA dan LFA. Hasil ini memberikan pemahaman lebih baik dan komprehensif mengenai peranan penting gen SLC23A3 terhadap komposisi asam lemak pada hati dan lebih menerangkan secara jelas kandidat seleksi berbasis genomik pada ayam terkait komposisi asam lemak.Kata kunci: keragaman, ekspresi mRNA, SLC23A3, seleksi, asam lemak tak jenuh ABSTRACT SLC23A3 is one of the key genes which control the properties of the of fatty acids content in the meat. The aim of this study was to identify the association of SNP and mRNA expression of SLC23A3 as a candidate marker for fatty acid traits (FAs). Sixty two F2 Kampung × Broilers crossbred chickens population were used in this study. FA composition was measured at 12 weeks of age from thigh meat of crossbred chicken. The PCR-RFLP and qRT-PCR were used for association study and expression analysis of SLC23A3 gene with divergent fatty acid composition. A SNP in coding region c.22385690 A>C of the SLC23A3 gene was associated (P<0.05) with fatty acid composition including stearic acid (C18:0), elaidic acid (C18:1n9t), and linoleic acid (C18:2n6c). The SLC23A3 was detected in liver from SLC23A3 as Candidate Marker for Fatty Acid Traits in Crossbred Chickens (A. Gunawan et al.) 201 high fatty acids (HFA) and low fatty acid (LFA) composition. However, gene expression of SLC23A3 were not differentially expressed between HFA and LFA. These results will explain better understanding of the key important role of the SLC23A3 in fatty acid traits within the liver and will propose SLC23A3 as a potential genomic selection for selection of chickens with fatty acid composition.
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