Expressão de marcadores de proliferação celular e apoptose no carcinoma espinocelular de pele e ceratose actínica

Dornelas, Marilho Tadeu; Rodrigues, Michele Fernandes; Machado, Dequitier Carvalho; Gollner, Ângela Maria; Ferreira, Ana Paula

doi:10.1590/s0365-05962009000500004

Cited by 19 publications

(13 citation statements)

References 11 publications

(21 reference statements)

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“…Some authors evaluated the expression of PCNA, Ki-67, p53 and bcl-2 in patients with squamous skin carcinoma (n=10) and actinic keratosis (n=10), and confirmed the absence of Ki-67 expression in two cases, confirming the variability in this marker's immunoreactivity 18 . However, it is interesting to note that in several studies the Ki-67 presents a tendency to strong direct correlation with the lesion malignancy degree, being valuable as a prognostic predictor 17,18 .…”

Section: Discussionmentioning

confidence: 81%

“…The Ki-67 immunoreactivity relates to evidence of cell proliferation, so that it is expressed in all cell cycle phases but G0, in which cells are quiescent 18 . According to Sousa et al 19 , immunohistochemical analysis of this marker is an effective method for assessing the human malignancies growth fraction, providing valuable information about prognosis.…”

Section: Discussionmentioning

confidence: 99%

“…However, it is interesting to note that in several studies the Ki-67 presents a tendency to strong direct correlation with the lesion malignancy degree, being valuable as a prognostic predictor 17,18 . The p16 INK4a tumor suppressor gene, encoding the p16 protein, is inactivated by hypermethylation in several types of malignancies, including oral squamous cell carcinoma, constituting a crucial event in the early stages of malignant transformation of the affected tissue.…”

Section: Discussionmentioning

confidence: 99%

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Expression of Ki-67 and P16 INK4a in chemically-induced perioral squamous cell carcinomas in mice.

Alves

Ribeiro

Lima

et al. 2016

Rev. Col. Bras. Cir.

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Objective: to evaluate the influence of INK4a proteins immunohistochemical expressions on the clinical and morphological parameters of perioral squamous cell carcinoma induced with 9,10-dimethyl-1,2-benzanthracene (DMBA) in mice. Methods: we topically induced the lesions in the oral commissure of ten Swiss mice for 20 weeks, determining the time to tumors onset and the average tumor volume up to 26 weeks. In histopathological analysis, the variables studied were histological malignancy grade and the immunohistochemical expression of INK4a proteins. The correlation between variables was determined by application of the Spearman correlation test. Results: the mean time to onset of perioral lesions was 21.1 ± 2.13 weeks; mean tumor volume was 555.91 ± 205.52 mm3. Of the induced tumors, 80% were classified as low score and 20% high score. There was diffuse positivity for Ki-67 in 100% of lesions -Proliferation Index (PI) of 50.1 ± 18.0. There was a strong direct correlation between Ki-67 immunoreactivity and tumor volume (R = 0.702) and a low correlation with the malignancy score (R = 0.486). The P16INK4a protein expression was heterogeneous, showing a weak correlation with tumor volume (R = 0.334). There was no correlation between the immunohistochemical expression of the two proteins studied. Conclusion: in an experimental model of DMBA-induced perioral carcinogenesis, tumor progression was associated with the tumor proliferative fraction (Ki-67 positive cells) and with tumor histological grading, but not with P16 INK4a expression.Keywords: Carcinogenesis. 9,10-Dimetil-1,2-benzanthracene. Immunohistochemistry. Ki-67 Antigen. Genes, p16. MTSI, CDK4I or CDKN2) located on chromosome 9p, locus 21, involved in cell cycle progression blocking process. It is inactive in a wide range human malignancies. The loss of p16 INK4a immunoexpression has been observed in the early stages of oral carcinogenesis and has been considered a molecular event of significant value in the prognostic analysis of such tumors 9,10 . This study evaluated the influence of Ki-67 and p16 proteins immunohistochemical expression on morphological parameters (mean tumor volume and histological malignancy grade) of DMBA-induced perioral SCC in mice. In addition, it sought to verify the existence of correlation between the immunoreactivity of p16 and Ki-67 proteins. METHODSThe development of the study had the approval of the Ethics in Research Committee of the Universidade Tiradentes -Aracaju / SE, with protocol number 191208. Animals and chemical carcinogenesis induction procedureWe used ten Swiss mice without distinction between gender, from the vivarium of the Universidade Tiradentes, with a body mass of about 150 ± 30g (Average age 100 days).We induced the oral lesions in the mice left oral commissure by the topical application of 9,10-dimethyl-1,2-benzanthracene (DMBA), diluted to 0.5% in acetone, on a weekly basis in three alternate days for 20 weeks 11 . After this period, the animals were kept under observation for six weeks, and we duly regis...

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Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Expression of Ki-67 and P16 INK4a in chemically-induced perioral squamous cell carcinomas in mice.

Alves

Ribeiro

Lima

et al. 2016

Rev. Col. Bras. Cir.

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“…al, 2006). Studies by DORNELAS et al (2009) suggested that the prolonged stay of the cell in the G1 phase of the cycle may lead it to apoptosis, and also that the inappropriate output of the G1 phase forces the p53 protein to suppress cell growth more pronouncedly. Figure 4 shows the important role of p53 in maintaining the genome integrity, acting systematically against injuries to the cell genetic material.…”

Section: Mechanisms Of Action Of P53 In Neoplasiasmentioning

confidence: 99%

P53 gene: major mutations in neoplasias and anticancer gene therapy

et al. 2012

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“…2 The lethality of nonmelanoma skin cancer is considered low; however, in some cases in which diagnosis is delayed, this cancer may lead to ulcerations and severe physical deformity. 2,3 There is, almost certainly, considerable under-registration as a result of under-diagnosis and also because prognosis is excellent with this type of neoplasia and the likelihood of complete cure is high if the condition is treated adequately and opportunely. 2 The majority of skin cancers result from excess sun exposure.…”

Section: Introductionmentioning

confidence: 99%

Comparação entre a concentração de mastócitos em carcinomas espinocelulares da pele e da cavidade oral

Parizi¹,

Barbosa

Parizi

et al. 2010

An. Bras. Dermatol.

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FUNDAMENTS:The lethality of squamous cell carcinomas (SCC) of the skin is considered low. SCC in the mouth is usually associated with poor prognosis. Current evidence suggests that mast cells in the normal tissue contribute to the tumorigenesis of SCC, probably by promoting angiogenesis. OBJECTIVE: The aim of this study was to compare the concentration of mast cells in SCC of the mouth and skin and evaluate whether there is a correlation with the degree of differentiation of these tumors. MATERIAL AND METHODS: Thirty cases of SCC of the skin and 34 of the mouth were investigated. Toluidine blue staining was used to identify mast cells in blocks containing the central portion of the neoplasm. RESULTS: A concentration of between 0 and 10 mast cells was found in one single case of SCC of the skin and there were no cases of SCC of the mouth with concentrations of mast cells in the tumor >201. In the majority of cases of SCC of the mouth (47%; n=16), mast cell concentration was between 0 and 10, with a concentration >51 mast cells in 80% of cases of SCC of the skin. All the cases of SCC of the mouth with a concentration of mast cells between 100 and 200 and 80% of those with a concentration of 51-99 were located on the lip. The concentration of mast cells was unrelated to the degree of differentiation of the tumor. CONCLUSION: The concentration of mast cells is lower in SCC of the mouth except when the tumor is located on the lip. This may reflect a lower need for cell activation in the microenvironment to improve vascularization in oral cancer.

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Expressão de marcadores de proliferação celular e apoptose no carcinoma espinocelular de pele e ceratose actínica

Cited by 19 publications

References 11 publications

Expression of Ki-67 and P16 INK4a in chemically-induced perioral squamous cell carcinomas in mice.

Expression of Ki-67 and P16 INK4a in chemically-induced perioral squamous cell carcinomas in mice.

P53 gene: major mutations in neoplasias and anticancer gene therapy

Comparação entre a concentração de mastócitos em carcinomas espinocelulares da pele e da cavidade oral

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