Exploring Dose-Dependent Cytotoxicity Profile of Gracilaria edulis-Mediated Green Synthesized Silver Nanoparticles against MDA-MB-231 Breast Carcinoma

Abstract

Green-based synthesis of metal nanoparticles using marine seaweeds is a rapidly growing technology that is finding a variety of new applications. In the present study, the aqueous extract of a marine seaweed, Gracilaria edulis, was employed for the synthesis of metallic nanoparticles without using any reducing and stabilizing chemical agents. The visual color change and validation through UV-Vis spectroscopy provided an initial confirmation regarding the Gracilaria edulis-mediated green synthesized silver nanoparticles. The dynamic light scattering studies and high-resolution transmission electron microscopy pictographs exhibited that the synthesized Gracilaria edulis-derived silver nanoparticles were roughly spherical in shape having an average size of 62.72 ± 0.25 nm and surface zeta potential of - 15.6 ± 6.73 mV. The structural motifs and chemically functional groups associated with the Gracilaria edulis-derived silver nanoparticles were observed through X-ray diffraction and attenuated total reflectance Fourier transform infrared spectroscopy. Further, the synthesized nanoparticles were further screened for their antioxidant properties through DPPH, hydroxyl radical, ABTS, and nitric oxide radical scavenging assays. The phycosynthesized nanoparticles exhibited dose-dependent cytotoxicity against MDA-MB-231 breast carcinoma cells having IC50 value of 344.27 ± 2.56 μg/mL. Additionally, the nanoparticles also exhibited zone of inhibition against pathogenic strains of Bacillus licheniformis (MTCC 7425), Salmonella typhimurium (MTCC 3216), Vibrio cholerae (MTCC 3904), Escherichia coli (MTCC 1098), Staphylococcus epidermidis (MTCC 3615), and Shigella dysenteriae (MTCC9543). Hence, this investigation explores the reducing and stabilizing capabilities of marine sea weed Gracilaria edulis for synthesizing silver nanoparticles in a cost-effective approach with potential anticancer and antimicrobial activity. The nanoparticles synthesized through green method may be explored for their potential utility in food preservative film industry, biomedical, and pharmaceutical industries.

Polianthes tuberosa-Mediated Silver Nanoparticles from Flower Extract and Assessment of Their Antibacterial and Anticancer Potential: An In Vitro Approach

“…After treatment with PTAgNPs, the A431 cells were re-suspended in ice-cold PBS [ 51 ]. In a glass slide, approximately, 10,000 cells in a volume of 100 mL were coated with a thin layer ona 1.0% ( w / v ) agarose frosted glass slide and covered with a coverslip and incubated in cold condition for 10 min.…”

Comparative assessment of the biological activity of the green synthesized silver nanoparticles and aqueous leaf extract of Perilla frutescens (L.)

“…7 a,b). Similarly, Mohanta et al 69 reported that the IC 50 values for biosynthesized GE-AgNP treatment were significantly lower in MDA-MB-231 cells than in L-929 cells after exposure for 24 or 48 h. Also, the biosynthesized AgNPs using Pyrostegia venusta leaf aqueous indicated significantly increased cytotoxic activity against COS-7 cells in vitro, with IC 50 value of 50.48 µg/mL 70 . Heidari et al 71 reported that Thymus vulgaris L. extract possesses less toxicity (70% cell mortality) on T47D breast cancer cells than biosynthesized AgNPs (90% cell mortality).…”

Biosynthesis Nanoparticles And Molecular Study To Detect Bacterial Isolated From Patients With Diabetic Foot Ulcers In Babylon Province

“…For determination of MIC were concentrations of ZnO NPs (31.255μg /mL, 62.5μg /mL, 125μg/mL, 250μg/mL, 500μg /mL) incubated all these tubes at 37°C for 24 hr, with dark conditions, MIC is a lowest ZnO nanoparticles concentration that inhibited completely the bacterial growth and can detected and determined the concentration of tube first one 125μg/mL with to be the Minimum inhibitory concentration [18].…”