Experimental model of traumatic ulcer in the cheek mucosa of rats

Abstract

Purpose:To establish an experimental model of traumatic ulcer in rat cheek mucosa for utilization in future alternative therapy studies. Methods: A total of 60 adult male rats (250 -300g) were used. Ulceration of the left cheek mucosa was provoked by abrasion using a nº 15 scalpel blade. The animals were observed for 10 days, during which they were weighed and their ulcers were measured. The histological characteristics were analyzed and scored according to the ulcer phase. In the statistical analysis, a value of p<0.01 was considered a statistically significant response in all cases. Results: During the five first days, the animals lost weight (Student t test, p<0.01). The ulcerated area receded linearly over time and was almost completely cicatrized after 10 days (ANOVA, Tendency posttest, p<0.0001). Groups on days 1, 2 and 3 days displayed similar results, but a decrease in scores were observed after the 4th day. Conclusion: The proposed cheek mucosa ulcer model in rats can be considered an efficient, low-cost, reliable, and reproducible method. Key words: Ulcer. Mouth Mucosa. Wound Heling. Rats. RESUMOObjetivo: Estabelecer um modelo experimental de úlcera traumática em mucosa jugal de ratos para utilização em futuros testes de terapias alternativas. Métodos: Foram utilizados 60 ratos, adultos, machos, pesando entre 250 a 300g. A ulceração na mucosa jugal esquerda foi provocada por meio da abrasão desta com uma lâmina de bisturi número 15. Os animais foram observados por um período de 10 dias, sendo estes pesados e suas escoriações mensuradas. As características histológicas foram analisadas e descritas adotando escores para comparação dos estágios da fase da úlcera. Na análise estatística um valor de p<0,01 foi considerado uma resposta estatisticamente significante em todos os casos. Resultados: Durante os cinco primeiros dias os animais perderam peso ("t" Student -p<0,01). A área da úlcera regrediu linearmente com o tempo, estando quase que completamente cicatrizada ao final de dez dias (ANOVA, pós-teste de Tendência -p<0,0001). Os grupos do 1º, 2º e 3º dias tiveram comportamento semelhante havendo uma diminuição dos escores a partir do 4º dia. Conclusão: O modelo de úlcera na mucosa jugal de ratos proposto pode ser considerado eficaz, apresentando reprodutibilidade confiável e baixo custo. Descritores: Úlcera. Mucosa Bucal. Cicatrização de Feridas. Ratos. -Acta Cirúrgica Brasileira -Vol. 26 (3) 2011Alves APNN et al.

Ketoprofen-loaded Eudragit electrospun nanofibers for the treatment of oral mucositis

“…On the second day, the inflammatory manifestations continued and a white fibrous tissue was formed in both groups ( Figure 10C and D) where the ulcer reached its developmental peak. 59 By day 6, there was a marked reduction in the severity of mucositis in the treated group, showing healing of ulcerations with mild Figure 10 Photographs of rabbit mucosal pouch: (A) normal control, (B) rabbit mucosa immediately after induction, (C) treated group at day 2, (D) nontreated group at day 2, (E) treated group at day 6 and (F) nontreated group at day 6. Notes: arrow in (B) indicates the presence of hemorrhagic areas and extensive ulcerations.…”

An experimental model of chemically-induced ulceration of the buccal mucosa of Mus musculus

“…Same results were also found from the previous studies related to oral mucosa ulceration. 6,7 Ulcer on the oral mucosa can be observed as the lesions with the disintegration and loss of the epithelial tissue. 15 These parameters were also applied in observing the ulceration in our study, besides clinical sign of inflammation (redness and swelling).…”

“…4 Besides the use of chemical agents, ulcer models can also be produced by mechanical trauma or by thermallyinduced trauma. 6,7 For example, De Barros Silva, et al created a model of a mechanical trauma-induced ulcer in a Wistar-type mouse using a no.15 scalpel knife to form an 8 mm-wide ulcer to the buccal mucosa. 8 Shortcomings of previous studies include the procedures for the formation of non-standardized ulcers as well as the choice and use of tools, the use of chemicals, and the means of exposure of the experimental animals.…”

Human keratinocyte (HaCaT) stimulation and healing effect of the methanol fraction from the decoction from leaf from Sideroxylon obtusifolium (Roem. & Schult.) T.D. Penn on experimental burn wound model

“…Histopathological changes were evaluated with optical microscopy (Olympus BX 51, Japan). The histopathological parameters were determined and scored from 0 to 4, where zero score corresponding: absence of ulcer (AU), remodeled connective tissue (RCT); score 1: AU, fibrosis (F), slight chronic inflammation (CI); score 2: presence of ulcer (PU), F, moderate CI; score 3: PU, chronic inflammation process (granulation tissue) and score 4: PU, acute process (dilated vessels, mixed inflammatory infiltrate with neutrophils) (Adapted from Cavalcante et al, 2011). Stained with picrosirius red (PSR) were performed to measure the collagen fibers in the connective tissue of burn wounds.…”