An experimental model of chemically-induced ulceration of the buccal mucosa of Mus musculus

Abstract

Background: Ulceration caused by chemical agents used in dental practice for in-office or home-used is a common event, resulting in discomfort and pain. Treatments for such conditions are still being developed, requiring extensive experiments both in vitro and in vivo studies. At present, a standardized experimental mouse model for mucosal ulceration caused by a chemical inducer to study the pathogenesis of ulceration and to develop medications for treatment of ulceration is still not available. The aim of this study was to create a chemically induced model of ulceration of the buccal mucosa of mice. Methods: An in vivo study model of ulceration using a total of 9 mice (Swiss Webster) was performed. All mice received 70% acetic acid application on the left buccal mucosa, while the right buccal mucosa received only saline. Clinical and histological observations of ulcer formation and healing were performed, including the presence of redness and swelling, ulcer diameter, bodyweight as well as epithelial disintegration, dilation of blood vessels, and infiltration of inflammatory cells. Results: Buccal mucosa application of 70% acetic acid generated ulcers on day 2, reached its peak on day 3 and recovered by day 14. The histological features of inflammation were also seen in the ulcer model, and the degree of inflammation was consistent with the day of ulcers. Conclusion: Chemical trauma by the administration of 70% acetic acid effectively induce ulceration on buccal mucosa in mice, and this method can be considered as a novel, reproducible, and clinically relevant model to study pathogenesis and therapeutic approach for treating oral mucosal ulceration.

Histological and Immunohistochemical Study of the Effect of Topically Applied Phenytoin on Chemically Induced Buccal Mucosal Ulcer in Albino Rats

“…These results are in accordance with Karavana et al [27] and Miao et al, [28] respectively. Moreover, Idrus et al, [29] reported complete healing of acetic acid induced ulceration of the buccal mucosa from 11 to 14 days and that was due to variation in healing capability and wound depth.…”

Anti-Inflammatory Activity and Wound Healing Effect of Kaempferia galanga L. Rhizome on the Chemical-Induced Oral Mucosal Ulcer in Wistar Rats

“…Induction of oral mucosal ulcer was using a cotton ball 10 mm in diameter, in a syringe without needle moistened with 70% acetic acid as much as 100 microliters, applied to the ventral of rat’s tongue for 2 minutes (modification from Kang and Kim, 2018; Miao et al, 2019; Idrus et al, 2019). 26 , 27 , 34 The induction technique was standardized for all animals and performed by the same operator.…”

Improved healing and macrophage polarization in oral ulcers treated with photobiomodulation (PBM)

“…An animal model of oral ulceration was used to observe the effect of PBM treatment. The method of induction was a modified version based on previously published animal models of chemically induced oral ulcers using acetic acid injections in the oral mucosa 29–31 . The animal experiments were carried out according to the guidelines set by the Institutional Animal Care and Use Committee at Dankook University (DK‐19‐046).…”

“…The method of induction was a modified version based on previously published animal models of chemically induced oral ulcers using acetic acid injections in the oral mucosa. [29][30][31] The animal experiments were carried out according to the guidelines set by the Institutional Animal Care and Use Committee at Dankook University (DK-19-046). Twelve 7-week-old Sprague-Dawley (SD) male rats weighing approximately 200-250 g (Orientbio Co.) were individually caged and acclimatized under laboratory conditions for 3 days before experimentation and provided with ad libitum food and water.…”

“…A chemically induced oral ulcer was selected as it allowed the creation of a consistent oral ulcer area in rats and was more relevant as a clinical stomatitis model. [29][30][31] Figure 2A shows the representative photographs of the chemically induced ulcers taken after each PBM treatment session. Gross examination shows that wound recovery was more evident in the treatment groups compared to the control group.…”