Human keratinocyte (HaCaT) stimulation and healing effect of the methanol fraction from the decoction from leaf from Sideroxylon obtusifolium (Roem. & Schult.) T.D. Penn on experimental burn wound model

Abstract

The larger number of plants, with therapeutic potential, popularly used in Northeastern Brazil is due to their easy access and the great Brazilian biodiversity. Previously, was demonstrated that the methanol fraction from Sideroxylon obtusifolium (MFSOL) promoted an anti-inflammatory and healing activity in excisional wounds. Thus, this work aimed to investigate the healing effects of MFSOL on human keratinocytes cells (HaCaT) and experimental burn model injuries. HaCaT cells were used to investigate migration and proliferation of cell rates. Female Swiss mice were subjected to seconddegree superficial burn protocol and divided into four treatment groups: Vehicle (cream-base), 1.0% Silver Sulfadiazine (Sulfa), and 0.5% or 1.0% MFSOL cream (CrMFSOL). Samples were collected for quantification of the inflammatory mediators and histological analyses after 3, 7 and 14 days on evaluation. As result, MFSOL (50 μg/ml) stimulated HaCaT cells by increasing proliferation and migration rates.Moreover, CrMFSOL 0.5% attenuated myeloperoxidase (MPO) activity and also stimulated the release of IL-1β and IL-10, after 3 days with treatment. CrMFSOL 0.5% enhanced wound contraction, promoted tissue remodeling improvement and highest collagen production after 7 days, and VEGF release after 14 days. Therefore, MFSOL evidenced the stimulation of human keratinocyte (HaCaT) cells and improvements on wound healing via inflammatory modulation on burn injuries.

Synthesis, characterization, and wound healing properties of α/γ hybrid peptides

“…Various research documented the migration of keratinocytes at the wound site as the early and important factor behind refurbishing of the wound. [30][31][32][33][34][35][36][37] Considering the higher cell viability of S1 and S2 against HaCaT and RAW 264.7 cells, we proceeded with further studies with these peptides. We performed in vitro cell scratch assay in order to investigate the effect of S1 and S2 on keratinocyte migration at 10 μg/ml concentration.…”

“…The revamping of the wound starts with stimulation of dermal fibroblasts, which are activated due to platelet‐derived growth factor (PDGF) and TGF‐β secreted by keratinocytes. Various research documented the migration of keratinocytes at the wound site as the early and important factor behind refurbishing of the wound 30–37 …”