Experimental assessment of small intestinal submucosa as a bladder wall substitute

Intestinal regeneration by a novel surgical procedure

“…Vacanti's group, using polyglycolic acid scaffolds seeded with intestinal epithelial organoid units harvested from neonatal rats, successfully obtained an enteric cyst, which has similar mucosal architecture and basic physiology to that of native bowel but lacks the normal tubular appearance and properly oriented muscle 13 . Porcine small intestine submucosa has been widely studied for use in arterial and venous grafts 14,15 , and to repair the urinary bladder 16 , ligaments and tendons 17 , and defects in the body wall 18 . Chen and Badylak 19 produced three regenerated layers of intestine using a patch of small intestine submucosa in a canine model, but they failed to produce regeneration with a tube of small intestine submucosa.…”

Aspects of Urological Tissue Engineering

“…(Atala, 1993(Atala, , 1998 Concurrently, studies by Knapp et al and others were evaluating the use of small intestinal submucosa (SIS) as an alternative biological scaffold for bladder tissue engineering. (Knapp, 1994;Kropp, 1995) The results from these studies seemed to suggest that SIS was a suitable biological material for the bladder augmentation process. However, in two separate studies by Ashley et al, unseeded SIS would be found to be highly pro-inflammatory.…”

Tissue-Engineered Extracellular Matrices (ECMs) as Adjuvant Scaffolds for Endovascular Aneurysmal Repair (EVAR)

“…Reparation of ureteral defect Porcine SIS graft replaced with urothelium and smooth muscle after 9 weeks B.P. Kropp (1995) Urinary bladder regeneration Rat Urothelium, lamina propria and smooth muscle replaced within 3 weeks (Ahn et al 2007, Cimini et al 2005, Freytes et al 2008, Hodde et al 2002, Roeder et al 1999, Sellaro et al 2007, Teebken et al 2000. These characterisation methods have led to a greater understanding of an ECM's biological, structural and mechanical properties.…”