Intestinal regeneration by a novel surgical procedure

Jwo, Shyh-Chuan; Chiu, Jen‐Hwey; Ng, Koon‐Kwan; Chen, H.-Y.

doi:10.1002/bjs.6069

Cited by 8 publications

(9 citation statements)

References 23 publications

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“…In addition to the benefits described above, our unique model for generating neointestine will enable accurate comparative studies between neointestinal regeneration and intestinal adaptation to be conducted in future investigations. This new model is also superior to our previous model in that it reduces observational interference in neointestinal regeneration. Because regenerated tissues can be easily identified at both ends of donor intestine in the central location of a silastic tube and also in a relatively isolated space near to the liver surface, it should prevent measurement bias from central scar contracture in end healing or peripheral tissue adhesions.…”

Section: Discussionmentioning

confidence: 99%

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A novel model for simultaneous study of neointestinal regeneration and intestinal adaptation

Jwo

Tang

Chen

et al. 2013

Wound Repair Regeneration

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The use of autologous grafts, fabricated from tissue-engineered neointestine, to enhance insufficient compensation of intestinal adaptation for severe short bowel syndrome is a compelling idea. Unfortunately, current approaches and knowledge for neointestinal regeneration, unlike intestinal adaptation, are still unsatisfactory. Thus, we have designed a novel model of intestinal adaptation with simultaneous neointestinal regeneration and evaluated its feasibility for future basic research and clinical application. Fifty male Sprague-Dawley rats weighing 250-350 g underwent this procedure and sacrificed at 4, 8, and 12 weeks postoperatively. Spatiotemporal analyses were carried out by gross, histology, and DNA/protein quantification. Three rats died of operative complications. In early experiments, the use of hard silicone stent as tissue scaffold in 11 rats was unsatisfactory for neointestinal regeneration. In later experiments, when a soft silastic tube was used, the success rate increased up to 90.9%. Further analyses revealed that no neointestine developed without donor intestine; regenerated lengths of mucosa and muscle were positively related to time postsurgery but independent of donor length with 0.5 or 1 cm. Other parameters of neointestinal regeneration or intestinal adaptation showed no relationship to both time postsurgery and donor length. In conclusion, this is a potentially important model for investigators searching for solutions to short bowel syndrome.

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Section: Discussionmentioning

confidence: 99%

“…However, neointestinal regeneration is complex and still in its infancy. Various experimental models for tissue‐engineered neointestine have been developed, but no ideal model has yet been identified. This may be attributed to our lack of understanding of the key factors involved in neointestinal regeneration apart from intestinal adaptation.…”

mentioning

confidence: 99%

A novel model for simultaneous study of neointestinal regeneration and intestinal adaptation

Jwo

Tang

Chen

et al. 2013

Wound Repair Regeneration

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“…Harmon and colleagues used Dacron as a patch to repair defects in the ileum of rabbits (Harmon et al, 1979). Although such studies on the use of prosthetic materials as a patch for repair of bowel defects were initially thought to be unsuccessful because the materials used were non-resorbable, the use of non-resorbable materials for studying intestinal morphogenesis and regeneration continues to be of interest (Jwo et al, 2008). Although such studies on the use of prosthetic materials as a patch for repair of bowel defects were initially thought to be unsuccessful because the materials used were non-resorbable, the use of non-resorbable materials for studying intestinal morphogenesis and regeneration continues to be of interest (Jwo et al, 2008).…”

Section: Small Intestinementioning

confidence: 99%

“…Intestinal regeneration occurred but muscle regeneration in an organized manner was not observed. An alternative model using dysfunctioned bowel has been used by Jwo and colleagues that involved grafting a 3 cm silicone tube into the bowel after Roux-en-Y bypass surgery (Jwo et al, 2008). The tubular graft was interposed in the middle of a Thiry-Vella loop (a defunctionalized segment of ileum that is brought out as a double ileostomy) in Lewis rats.…”

Section: Small Intestinementioning

confidence: 99%

Alimentary Tract

Day

2011

Principles of Regenerative Medicine

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“…Using regenerative medicine, many organs and tissues are expected to be manufactured by tissue engineering [9–11]. Various experimental models for tissue-engineered neointestine have been developed [12–17], but no ideal model has yet been identified. Previous studies focused on either intestinal adaptation [2, 4, 18–21] or intestinal transplantation [22–25] alone.…”

Section: Introductionmentioning

confidence: 99%

Identifying the Growth Factors for Improving Neointestinal Regeneration in Rats through Transcriptome Analysis Using RNA-Seq Data

Jwo

Chung

Wang³

et al. 2018

BioMed Research International

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Using our novel surgical model of simultaneous intestinal adaptation “A” and neointestinal regeneration “N” conditions in individual rats to determine feasibility for research and clinical application, we further utilized next generation RNA sequencing (RNA-Seq) here in normal control tissue and both conditions (“A” and “N”) across time to decipher transcriptome changes in neoregeneration and adaptation of intestinal tissue at weeks 1, 4, and 12. We also performed bioinformatics analyses to identify key growth factors for improving intestinal adaptation and neointestinal regeneration. Our analyses indicate several interesting phenomena. First, Gene Ontology and pathway analyses indicate that cell cycle and DNA replication processes are enhanced in week 1 “A”; however, in week 1 “N”, many immune-related processes are involved. Second, we found some growth factors upregulated or downregulated especially in week 1 “N” versus “A”. Third, based on each condition and time point versus normal control tissue, we found in week 1 “N” BMP2, BMP3, and NTF3 are significantly and specifically downregulated, indicating that the regenerative process may be inhibited in the absence of these growth factors. This study reveals complex growth factor regulation in small neointestinal regeneration and intestinal adaptation and provides potential applications in tissue engineering by introducing key growth factors identified here into the injury site.

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Intestinal regeneration by a novel surgical procedure

Cited by 8 publications

References 23 publications

A novel model for simultaneous study of neointestinal regeneration and intestinal adaptation

A novel model for simultaneous study of neointestinal regeneration and intestinal adaptation

Alimentary Tract

Identifying the Growth Factors for Improving Neointestinal Regeneration in Rats through Transcriptome Analysis Using RNA-Seq Data

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