Examination of denture‐cleaning methods based on the quantity of microorganisms adhering to a denture

Abstract

Examination of denture-cleaning methods based on the quantity of microorganisms adhering to a denture Objectives: To investigate effective denture-cleaning methods, we examined the relationships between the quantity of microorganisms adhering to dentures and the use of a denture brush and the frequency of use of a denture cleanser. Subjects and Methods: Denture plaque was collected from the mucosal surface of the examined dentures, which were 142 and 80 upper and lower complete dentures, respectively, worn by 96 outpatients (mean age: 71.9 years) of a university hospital and 41 nursing home residents (mean age: 84.8 years). The collected microorganisms were counted in terms of isolated representative colonies that were cultured and identified using standard methods. The use of a denture brush, the frequency of use, and the type and soaking time of denture cleansers as denture-cleaning methods were surveyed. Results: The quantity of microorganisms was significantly lower in dentures of denture brush users than in those of non-users in the outpatients (p < 0.01, Mann-Whitney U test). The quantity of microorganisms was significantly lower in the dentures of outpatients who used a denture cleanser daily or 3-4 times a week than in those who used one once or less per month and in the dentures of nursing home residents who used one daily than in those who used one at other frequencies (p < 0.05, Kruskal-Wallis test, followed by Dunn's Multiple Comparison test). Conclusion: Within the limitations of this study, it was concluded that the use of a denture brush and daily use of denture cleanser should be recommended to complete dentures wearers as denture-cleaning methods that effectively reduce the quantity of microorganisms adhering to dentures.

Dry mouth and denture plaque microflora in complete denture and palatal obturator prosthesis wearers

“…In terms of the quality of the oral microbial flora, the predominant microorganisms in this study were Streptococcus spp., Neisseria spp. and Candida spp., which is consistent with other results. The prevalence of Streptococcus spp., which were the most common microorganisms in this study, showed no significant differences depending on the type of prosthesis or presence of dry mouth.…”

“…However, pouring specific culture media into a denture or impression allows for examination of only particular microorganisms, such as Candida spp. Therefore, examination of microbial flora is performed using a swab or spatula. In terms of the sampling area, Kashiwabara et al .…”

“…Nishi et al . reported that the number of microorganisms adhering to dentures is related to the denture‐cleaning method used. Therefore, further research will be required regarding differences among denture‐cleaning methods.…”

In Vitro Antifungal Evaluation of Seven Different Disinfectants on Acrylic Resins

“…Nishi et al [38] reported that daily soaking of dentures in a denture cleanser was effective method for reducing the quantities of microorganisms adhering to dentures. The guidelines outlined by the American College of Prosthodontics recommend that dentures should be cleaned daily by soaking and brushing with on effective, nonabrasive denture cleanser [39].…”

Antifungal, optical, and mechanical properties of polymethylmethacrylate material incorporated with silanized zinc oxide nanoparticles

“…1 Daily denture brushing is recommended to reduce the amount of microorganisms adhering to the denture surface. 2 However, oral candidiasis can still occur in patients with predisposing factors, such as immunocompromised status, xerostromia, and age of the denture. 1 Moreover, advancing age is also a risk factor for dentureinduced stomatitis in elderly patients due to their reduced cell-mediated immunity that may lead to fungal infection.…”