Ex vivo studies of polymorphonuclear neutrophils from patients with early‐onset‐periodontitis (II). Chemiluminescence response analysis

Abstract

Previous studies have indicated that oxygen-free radicals may cause damage to the periodontal tissues. This study compared the luminol-dependent chemiluminescence response (after stimulation with either opsonized zymosan or phorbol myristate acetate (PMA) of peripheral blood polymorphonuclear leukocytes (PMN) isolated from human subjects with a healthy periodontium (n = 7), gingivitis (n = 8), adult periodontitis (n = 8), or early-onset periodontitis (n = 17). These results were also compared with those obtained in a larger reference group which consists of 50 subjects without infection or inflammation, selected on the basis of laboratory investigations. An enhanced response was defined as being 2 standard deviations above the reference group mean; a reduced response was defined as being 2 standard deviations below this mean. Although PMN from patients with either gingivitis or periodontitis were often functionally activated (when compared to the PMN from the reference group), no significant differences could be found between the 4 groups, with regard to the chemiluminescence response means obtained in a basal state or after stimulation.

Expression of the α‐subunit of CR4(CD11c) by peripheral blood and crevicular fluid neutrophils

“…No significant difference could be found between the groups, neither for the percentages of positive PB-PMNs or CF-PMNs, nor for the expression of the a subunit of CR4 {Kruskall and Wallis non-parametric test). These results were not surprising, since all the subjects were in good health, and since their PB-PMNs were perfectly functional as much for their mobihty behavior as for their oxidative metabolism (4,5). Similar results have been obtained earlier for LFA-1 and CR3 (6).…”

Oxidative Injury and Inflammatory Periodontal Diseases : the Challenge of Anti-Oxidants to Free Radicals and Reactive Oxygen Species

“…PMN from both PLS patients and controls produced similar amounts of 02'-when stimulated with opsonized bacteria or PMA, but both resting PMN and PMN stimulated with polyhistidine from the PLS patients released larger amounts of 02' than PMN from the controls (Bimstein et al, 1990). In AP, only a slight increase in CL was observed (Whyte et al, 1989), while there were minor differences (Zafiropoulos et al, 1991), no differences (Mouynet et al, 1994), or a CL decrease (Gomez et al, 1994) among healthy subjects or patients with gingivitis, juvenile periodontitis, or AP PMN from AP patients have higher CL after Fcgreceptor stimulation than do those from healthy subjects (Gustafsson and Asman, 1996). In RPP patients was found a lowering of CL (Zafiropoulos et al, 1988), and only minor differences in the CL responses between PE patients and healthy controls were found in terms of the oxidative metabolism of PMN (Zafiropoulos et al, 1991).…”

Constitutionally Hyperreactive Neutrophils in Periodontitis

“…This agrees with our previous findings and those of others (Table 1). [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] We tried to avoid possible interference by inflammation with a careful examination to confirm good general health and excluding cigarette smokers to clarify the pathogenesis of periodontitis.…”

“…However, Fcγ-receptor (FcγR)-mediated stimulation has consistently shown an increase in the generation of oxygen radicals from activated neutrophils of patients with periodontitis (Table 1). [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] The primary aim of this study was to compare the neutrophil generation of oxygen radicals from patients with chronic periodontitis and matched controls after stimulation via various activating pathways. We therefore activated the neutrophils via FcγR (IgG-opsonized Staphylococcus aureus), complement receptor CR3, (complement-opsonized zymosan), and directly using activation of the intra-cellular protein kinase C enzyme (PKC) by phorbol myristate acetate (PMA).…”