Evaluation the Safety of Thymoquinone Loaded on Gold Nanoparticles in the Treatment of Hamster Buccal Carcinogenesis

El-Mansy, Merhan N.; Hassan, Magda; El-Nour, Kholoud M. Abou; El-Hosary, Wafaa H

doi:10.21608/scumj.2017.46086

Cited by 5 publications

(6 citation statements)

References 16 publications

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“…In the present study, GII revealed increase in total WBCs (14x10 3 cells/ µl) compared to that of GI (8x10 3 cells/ µl), this reflected by extremely significant difference (p-value < 0.001), this is in line with that reported by Khan et al (2022) (37) This indicated the high local & systemic toxicity of DMBA in the hamster animal model. (22,28,38) Similarly, Leucocytosis is prevalent in individuals with progressive OSCC. as Tumor-related leukocytosis this results explained hematopoietic colony-stimulating factors and inflammatory cytokines Production of cytokines, chemokines and granule proteins promotes, which promotes tumor growth, angiogenesis, and increase its metastasis potential.…”

Section: Discussionmentioning

confidence: 99%

“…Following that, the animals in GII (positive control) underwent no other medication, whereas those in GIII (cetuximab-3w) received cetuximab 1 mg/animal IP via insulin syringe at 3-day intervals for 3 weeks. (21,22) (Fig. 1B), whereas those in GIV (cetuximab-6w) were injected IP by insulin syringe with cetuximab 1 mg/animal at 3 days intervals for 6 weeks.…”

Section: Experimental Designmentioning

confidence: 99%

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Therapeutic efficacy of time-dependent cetuximab on experimentally induced hamster buccal pouch carcinoma

Shamia

Abd-Alhafez

Alqalshy

et al. 2022

ijhs

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Purpose: This study aimed to determine the cetuximab therapeutic potential in the time-dependent fashion in experimentally induced hamster buccal pouch (HBP) carcinoma. Material and methods: Forty Syrian male hamsters were classified into four equal groups (G) of ten each. GI: The animals remained untreated to act as negative controls. The right pouches of animals in GII, GIII, and GIV were painted three times a week for 14 weeks (w)s with 7,12-dimethylbenz (a) anthracene (DMBA). GII: No additional treatment was administered. While, the animals in GIII and GIV were treated differently. Those in GIII received cetuximab intraperitoneally (IP) three intervals a week for three (w)s, whereas those in GIV received cetuximab IP three intervals for six (w)s. After the end of the experiment, the gross observations were made, and blood samples (2ml) were withdrawn from the inner canthus of the eye for analysis of whole white blood cells (WBCs) and oxidative stress markers [glutathione (GSH) and malondialdehyde (MDA) levels]. All pouches were surgically bisected for preparation for Hematoxylin and Eosin (H&E) stain and immunohistochemistry (IHC) stain using epidermal growth factor receptor (EGFR). Other fresh tissue was used for DNA detection through a flow cytometry (FCM) test.

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Section: Discussionmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

Therapeutic efficacy of time-dependent cetuximab on experimentally induced hamster buccal pouch carcinoma

Shamia

Abd-Alhafez

Alqalshy

et al. 2022

ijhs

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“…Those in GIV (TQ G): The animals were injected IP by insulin syringe (Fig. 1C) with TQ 50 mg/kg thrice a week for 3 ws (58) .…”

Section: Experimental Designmentioning

confidence: 99%

Chemopreventive and therapeutic efficacy of thymoquinone on experimentally induced hamster buccal pouch carcinogenesis

Shamia

Abd-Alhafez

Soliman³

et al. 2022

ijhs

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Aim: The present study aimed to investigate the chemopreventive and therapeutic effect of thymoquinone (TQ) on experimentally induced hamster buccal pouch carcinogenesis. Methodology : 40 Syrian male hamsters were classified into 4 equal groups (G(s)) of 10 each. GI: The animals remained untreated. The pouches of animals in GII, GIII, and GIV were painted 3 times a week for 14 weeks (ws). with 7,12-dimethylbenz (a) anthracene (DMBA). GII: No additional treatment was administered. Those in GIII: oral administration of TQ at a dose of 30 mg kg-1, one week before, as well as, during the application of DMBA on alternate days for 14 ws, whereas in GIV: The animals were injected intraperitoneally (IP) with TQ 50 mg/kg body weight thrice a week for 3ws. After the end of the experiment, the gross observations were made. Then, the fresh pouch specimens were surgically bisected into two pieces, one for Hematoxylin and Eosin (H&E) stain and immunohistochemistry (IHC) stain using Bcl-2. The other piece was used for apoptosis detection through a flow cytometry (FCM) test. Results: Gross observation showed variation in reduction of the tumor size in chemoprevention and treated group compared to the DMBA treated group.

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“…• Sub-group B3: Six animals were injected intraperitoneal with 0.001 GNPs-TQ/3 times/ week for six weeks 19 .…”

Section: Experimental Designmentioning

confidence: 99%

A trial of thymoquinon loaded on gold nanoparticle as a therapy for induced squamous cell carcinoma in buccal pouch of hamster

Shata

Taha

2019

Egyptian Dental Journal

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Objectives: Combining the anti-tumor effect of thymoquinone (TQ) with the efficient penetration of gold nanoparticles (GNPs) into cells and nuclei as an attempt to treat the induced oral squamous cell carcinoma in buccal pouch of hamster. Materials and methods: This study was carried out on forty two male Syrian golden hamsters (n=42), its age ranged from 6 to 7 weeks, weighting 90-110 gm. Animals were housed with controlled temperature and were given pellets formed of seeds, grain, cracked corn and tap water ad libitum. The hamsters were divided into: Group A: (Control groups) n=24, Group A1: eighteen animals (n=18) were served as "negative control". Subgroup A1a: Six animals were sacrificed at day zero. Subgroup A1b: Six animals were sacrificed at 14th week. Subgroup A1c: Six animals were sacrificed at 20th week. Group A2: Six animals (n=6) were considered as "positive control". DMBA was painted to the left cheek pouches, three times /week for 14weeks. Group B: Eighteen animals (n=18) were painted with DMBA to left cheek pouches three times/week for 14weeks, and subdivided into: Subgroup B1: Six animals were injected intra-peritoneal with GNPs/3 times/week for six weeks. Subgroup B2: Six animals were injected intra-peritoneal with TQ/3 times/week for six weeks.Sub-group B3: Six animals were injected intra-peritoneal with GNPs-TQ/3 times/ week for six weeks. All pouches were extracted and prepared to examined through histological examination for any structural changes and immunohistological detection of COX2. Results: Improve superior anti-inflammatory role of TQ when loaded with GNPS in COX-2 retardation and tumor size regression. Conclusion: Thymoquinon loaded on nanogold particle showed protective role in the oral cancer.

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Evaluation the Safety of Thymoquinone Loaded on Gold Nanoparticles in the Treatment of Hamster Buccal Carcinogenesis

Cited by 5 publications

References 16 publications

Therapeutic efficacy of time-dependent cetuximab on experimentally induced hamster buccal pouch carcinoma

Therapeutic efficacy of time-dependent cetuximab on experimentally induced hamster buccal pouch carcinoma

Chemopreventive and therapeutic efficacy of thymoquinone on experimentally induced hamster buccal pouch carcinogenesis

A trial of thymoquinon loaded on gold nanoparticle as a therapy for induced squamous cell carcinoma in buccal pouch of hamster

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