Evaluation of the use of fibrin and microcrystalline chitosan membranes as carriers for transforming growth factor beta‐1

Abstract

The aim of this study was to describe the mechanical features of homogeneous and layered chitosan (Ch) and fibrin–chitosan (Fb–Ch) membranes as well the kinetics of transforming growth factor beta‐1 (TGF‐β1) release from five types of polymer carriers. Composites in the form of a film containing physiologically clotted fibrin (Fb) and microcrystalline chitosan (MCCh) were prepared and then crosslinked with calcium chloride. The films were characterized by Infrared (IR) spectroscopy, mechanical tests (film thickness, maximal tensile force, breaking strength, and elongation at break), and SEM images. The results reveal that Ch film demonstrates higher efficiency in binding TGF‐β1 and, at the same time, is less effective in its release—1.25% of the total amount between 6 h and 14 days. However, the Fb membrane binds TGF‐β1 not as strongly, which leads to more effective release of the compound—25% after 6 h and 28.98% of the total amount after 14 days. The factor TGF‐β1 is released in vitro from Fb–Ch membranes with different kinetics. The most efficient release of TGF‐β1 was observed in the case of the layered Fb–Ch (M4 L) membrane (after 14 days it reached a maximal value of 14.08% of the total amount). The release was lower with increasing Ch concentrations in the film, suggesting a high affinity of TGF‐β1 with the Fb–Ch component. The Fb–Ch membrane with incorporated TGF‐β1 may prove to be a very useful scaffold in the tissue regeneration process. This study demonstrates that Fb and MCCh gels could be used as carrier matrices for the controlled release of bioactive TGF‐β1. It was found that the degree of TGF‐β1 release from the membrane is influenced by the physiochemical and mechanical characteristics of the films and by its affinity to growth factor TGF‐β1. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013

Evaluation of Microcrystalline Chitosan and Fibrin Membranes as Platelet-Derived Growth Factor-BB Carriers with Amoxicillin

“…For comparison, one set of polymer membranes was prepared with MCCh (M1) in the absence of Fb, whereas another was prepared with Fb (M2) in the absence of MCCh. The method of film preparation was modified in comparison with our previous publication [42]. The polymer hydrogel was poured into the middle of the round metal disc ( = 40 mm, ℎ = 2 mm) placed on a Teflon plate.…”

“…The varied release of growth factors (b-FGF, TGF-, and PDGF-BB) from polymer carriers observed in our earlier studies [41][42][43] has been the reason of the interest in the kinetics of the release of PDGF-BB selected from natural biodegradable polymers (MCCh and Fb) in MCCh-Fb membranes. PDGF-BB loaded chitosan may be beneficial to enhance, for example, periodontal bone regeneration [6].…”

“…In our previous research Fb, MCCh, and methylcellulose (MC) hydrogels for bFGF in the presence of ketoprofen [41], Fb, and MCCh membranes for TGF- [42], MCCh, and collagen (Coll) membranes for PDGF-BB in the presence of amoxicillin (Am) [43] as carriers were studied. Previous studies did not include the impact of membrane structure on the sorption property and degree of release of growth factors.…”

Peptide-Directed Self-Assembly of Functionalized Polymeric Nanoparticles. Part II: Effects of Nanoparticle Composition on Assembly Behavior and Multiple Drug Loading Ability

“…Self‐assembled peptide nanofiber scaffolds have gained in popularity compared to scaffolding from other polymers, possibly because they are perceived as non‐toxic and biodegradable, and the porous fibril structures are thought to be similar to extracellular matrix (ECM) and so will aid cell attachment . Also, these materials can assemble in situ into a hydrogel at physiological environment .…”

Evaluation of the use of microcrystalline chitosan and collagen membranes as carriers for the platelet derived growth factor (PDGF-BB) in the presence of amoxicillin

“…Chi to san was cho sen as a bio medi cine be cause of its bio com pati bil ity, bio de grad abil ity, and non tox ic ity [4,12]. In our study, we used mi cro crys tal line chi to san (MCCh) in the form of hy dro gel at a neu tral pH of 6.8, which is used in the mem brane as a car rier for growth factors [10]. MCCh is a spe cial mul ti func tional poly meric ma te rial pre pared by the ag gre ga tion of glu co samine mac ro mole cules from an aque ous so lu tion of or ganic acid [15].…”