Evaluation of the use of microcrystalline chitosan and collagen membranes as carriers for the platelet derived growth factor (PDGF-BB) in the presence of amoxicillin

Abstract

In re cent years tis sue en gi neer ing (TI) has seen wide use in the treat ment of in jured bone, nerve and skin tissues. Natu ral, bio de grad able poly mers such as fi brin, gela tin, col la gen, chi to san ap peared to be the most use ful, pro vid ing fully con trolled bio com pati ble fac tor re lease [6,9,11]. Growth fac tors be long to the group of cy to ki nes, which bind with spe cific cel lu lar mem brane re cep tors. A sig nifi cant role in TI is played by the plate let de rived growth fac tor (PDGF), which is a di meric gly co pro tein en gaged in the regu la tion of cel lu lar di vi sion, mi gra tion or cel lu lar growth dur ing angio gene sis [5]. Cur rently available evi dence sup ports the use of PDGF-enhanced ma tri ces to pro mote perio don tal and peri-implant bone regen era tion. The use of growth fac tors such as PDGF-BB with bio com pati ble ma tri ces to pro mote tis sue re generation rep re sent a prom is ing ap proach in the dis ci plines of oral and max il lo fa cial sur gery. The re sults of pre clini cal and clini cal hu man stud ies evalu at ing the ef fec tive ness of growth-factor-enhanced ma tri ces con firmed the use fulness of PDGF in skele tal sur gery, such as oral sur gery [7,12,14].The pre ven tion of in fec tion oc cur rence and patho logical le sions re sult ing from mem brane im plan ta tion in side an or gan ism still re mains an im por tant prob lem. Pre viously data in di cated that the pro phy laxis of an ti bi otic us age in mem branes de creases the risk of in fec tion by even 81%. The drugs most fre quently used in mem branes are an ti bi ot ics and che mo thera peu tics [2].Col la gen and chi to san are highly bio com pati ble and pos sess fa vor able phys ico chemi cal prop er ties for this pur pose. Mo lecu lar in ter ac tions be tween col la gen and chi to san have the po ten tial to pro duce bio com pos ites with novel prop er ties [13]. Col la gen and chi to san do not ex ist to gether as blends in na ture, but the spe cific prop er ties of

Evaluation of Microcrystalline Chitosan and Fibrin Membranes as Platelet-Derived Growth Factor-BB Carriers with Amoxicillin

“…Analysis of the diagrams depicted reveals that two different release phases may be found. The obtained data indicates (Figures 8 and 9) that the release process of PDGF-BB from the membranes as described previously [43] can be written as the first order equation with two exponential functions:…”

“…Profiles of PDGF-BB release (Figures 8 and 9) revealed that the growth factor release is two-stage process with the initial faster effect and slower second stage [6,43,49].…”

“…The varied release of growth factors (b-FGF, TGF-, and PDGF-BB) from polymer carriers observed in our earlier studies [41][42][43] has been the reason of the interest in the kinetics of the release of PDGF-BB selected from natural biodegradable polymers (MCCh and Fb) in MCCh-Fb membranes. PDGF-BB loaded chitosan may be beneficial to enhance, for example, periodontal bone regeneration [6].…”

“…In our previous research Fb, MCCh, and methylcellulose (MC) hydrogels for bFGF in the presence of ketoprofen [41], Fb, and MCCh membranes for TGF- [42], MCCh, and collagen (Coll) membranes for PDGF-BB in the presence of amoxicillin (Am) [43] as carriers were studied. Previous studies did not include the impact of membrane structure on the sorption property and degree of release of growth factors.…”

Influence of General Mineral Condition on Collagen-Guided Alveolar Crest Augmentation

“…It requires scaffolding made of biomaterial on which a new bone is formed, gradually filling the bone defect, e.g., the alveolus. Scaffolding can be built of amorphous calcium phosphate, calcium carbonate, biologically active glass, crystalline calcium phosphate, fluorohydroxyapatite, collagen, or a mixture of collagen and calcium phosphate [10][11][12]. These are usually highly porous granules that are easily spilled in the operating field, but collagen is available in the form of a sponge that can easily be stabilized in the alveolus; hence, there is interest in the collagen-guided augmentation of the alveolar crest.…”