Evaluation of the Sensitivity of Freshwater Organisms Used in Toxicity Tests of Wastewater from Explosives Company

Ribeiro, Elaine Nolasco; Silva, Flávio Teixeira da; Paiva, Teresa Cristina Brazil de

doi:10.1007/s00128-012-0772-y

Cited by 4 publications

(2 citation statements)

References 12 publications

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“…Nevertheless, there are still concerns regarding the environmental and health impacts from exposure as well as the harmful by-products of these explosives. For example, long-term exposure to TNT in water and soil potentially affects the development of Daphnia similis and the annelid Enchytraeus crypticus (potworm) (Kuperman et al 2013 ; Ribeiro et al 2012a , b ). In addition, TNT and RDX are also toxic to mammals and have been regarded as dangerous chemical mutagens and potential human carcinogens (Chatterjee et al 2017 ; Serrano-González et al 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of a novel bacterium, Pseudomonas sp. ZyL-01, involved in the biodegradation of CL-20

Dang

Li³

et al. 2020

AMB Expr

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Hexanitrohexaazaisowurtzitane (CL-20) is a compound with a polycyclic cage and an N-nitro group that has been shown to play an unfavorable role in environmental fate, biosafety, and physical health. The aim of this study was to isolate the microbial community and to identify a single microbial strain that can degrade CL-20 with desirable efficiency. Metagenomic sequencing methods were performed to investigate the dynamic changes in the composition of the community diversity. The most varied genus among the microbial community was Pseudomonas, which increased from 1.46% to 44.63% during the period of incubation (MC0–MC4). Furthermore, the new strain was isolated and identified from the activated sludge by bacterial morphological and 16s rRNA sequencing analyses. The CL-20 concentrations decreased by 75.21 μg/mL and 74.02 μg/mL in 48 h by MC4 and Pseudomonas sp. ZyL-01, respectively. Moreover, ZyL-01 could decompose 98% CL-20 of the real effluent in 14 day’s incubation with the glucose as carbon source. Finally, a draft genome sequence was obtained to predict possible degrading enzymes involved in the biodegradation of CL-20. Specifically, 330 genes that are involved in energy production and conversion were annotated by Gene Ontology functional enrichment analysis, and some of these candidates may encode enzymes that are responsible for CL-20 degradation. In summary, our studies indicate that microbes might be a valuable biological resource for the treatment of environmental contamination caused by CL-20 and that Pseudomonas sp. ZyL-01 might be a promising candidate for eradicating CL-20 to achieve a more biosafe environment and improve public health.

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Section: Introductionmentioning

confidence: 99%

Isolation and identification of a novel bacterium, Pseudomonas sp. ZyL-01, involved in the biodegradation of CL-20

Dang

Li³

et al. 2020

AMB Expr

View full text Add to dashboard Cite

show abstract

“…For example, long-term exposure to TNT in water and soil potentially affects the development of Daphnia similis and the annelid Enchytraeus crypticus (potworm) (Kuperman et al 2013;Ribeiro et al 2012a; Ribeiro et al 2012b). In addition, TNT and RDX are also toxic to mammals and have been regarded as dangerous chemical mutagens and potential human carcinogens (Chatterjee et al 2017;Serrano-González et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of a novel bacterium, Pseudomonas sp. ZyL-01, involved in the biodegradation of CL-20

Dang

et al. 2020

Preprint

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Hexanitrohexaazaisowurtzitane (CL-20) is a compound with a polycyclic cage and an N-nitro group that has been shown to play an unfavorable role in several aspects, including environmental fate, biosafety, and physical health. The aim of this study was to isolate the microbial community and to identify a single microbial strain that can degrade CL-20 with desirable efficiency. Metagenomic sequencing methods were performed to investigate the dynamic changes in the composition of the community diversity. The most varied genus among the microbial community was Pseudomonas , which increased from 1.46% to 44.63% during the period of incubation (MC0–MC4). Furthermore, the new strain was isolated and identified from the activated sludge by bacterial morphological and 16s rRNA sequencing analyses. The CL-20 concentrations decreased by 75.21 μg/mL and 74.02 μg/mL in 48 h by MC4 and Pseudomonas sp. ZyL-01, respectively. However, the initial speed of CL-20 degradation by MC4 was more rapid than that by ZyL-01. Finally, a draft genome sequence was obtained to predict possible degrading enzymes involved in the biodegradation of CL-20. Specifically, 330 genes that are involved in energy production and conversion were annotated by Gene Ontology functional enrichment analysis, and some of these candidates may encode enzymes that are responsible for CL-20 degradation. In summary, our studies indicate that microbes might be a valuable biological resource for the treatment of environmental contamination caused by CL-20 and that Pseudomonas sp. ZyL-01 might be a promising candidate for eradicating CL-20 to achieve a more biosafe environment and improve public health.

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Chironomus sancticaroli (Diptera, Chironomidae) as a Sensitive Test Species: Can We Rely on Its Use After Repeated Generations, Under Laboratory Conditions?

Corbi

Bernegossi

Moura

et al. 2019

Bull Environ Contam Toxicol

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Evaluation of the Sensitivity of Freshwater Organisms Used in Toxicity Tests of Wastewater from Explosives Company

Cited by 4 publications

References 12 publications

Isolation and identification of a novel bacterium, Pseudomonas sp. ZyL-01, involved in the biodegradation of CL-20

Isolation and identification of a novel bacterium, Pseudomonas sp. ZyL-01, involved in the biodegradation of CL-20

Isolation and identification of a novel bacterium, Pseudomonas sp. ZyL-01, involved in the biodegradation of CL-20

Chironomus sancticaroli (Diptera, Chironomidae) as a Sensitive Test Species: Can We Rely on Its Use After Repeated Generations, Under Laboratory Conditions?

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