Evaluation of the Measurement of Serum Cystatin C by an Enzyme-Linked Immunosorbent Assay for Humans as a Marker of the Glomerular Filtration Rate in Dogs

Miyagawa, Yuichi; Takemura, Naoyuki; Hirose, Hisashi

doi:10.1292/jvms.71.1169

Cited by 42 publications

(61 citation statements)

References 27 publications

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“…However, the cut off value is 1.05 μg mL -1 which means the immuno-pillar has enough sensitivity. 7 The absorbance in a 96-well microtiter plate also increased with increasing concentration of cCys-C.…”

Section: Resultsmentioning

confidence: 93%

“…Despite various techniques that have been developed to explore symptoms of renal failure in human serum, detecting these failures in cats still has not attracted much attention. 7 The causes of CKD are heterogeneous and rarely identified; however the commonly used indirect GFR markers, serum creatinine (sCr) and blood urea nitrogen (BUN), are not sufficiently sensitive or specific to detect early renal dysfunction. 2 The serum concentrations of these markers increase when approximately 75% of the functional renal mass is lost.…”

Section: Introductionmentioning

confidence: 99%

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Rapid Detection of Cat Cystatin C (cCys-C) Using Immuno-Pillar Chips

et al. 2016

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We demonstrated a rapid immunoassay for detection of cat cystatin C (cCys-C) which is an important marker for chronic kidney disease in cats, using immuno-pillar chips. The required amount of reagent solution is 200 times smaller than that for the conventional ELISA in the 96-well microplate (0.5 mu L versus 100 mu L). In addition, the total assay time in the proposed method is more than 12 times shorter than in the conventional method (20 min versus 240 min). The limit of detection in the new method of 3 ng mL(-1) is comparable to that of the conventional method (1 ng mL(-1)) and it is in the clinically relevant range

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Section: Resultsmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Rapid Detection of Cat Cystatin C (cCys-C) Using Immuno-Pillar Chips

et al. 2016

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“…Neoplasias não interferem no resultado da cistatina C (MIYAGAWA et al 2009). Os poucos fatores extras renais, descritos em humanos, que interferem na sua mensuração, ocorre nas desordens da tireóide.…”

Section: Cistatina Cunclassified

Conceitos E Aplicabilidade Dos Principais Biomarcadores Na Nefrologia Veterinária – Revisão De Literatura

et al. 2017

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Concepts and applicability of main biomarkers in veterinary nephrology -literature review RESUMO ABSTRACTOs biomarcadores são substâncias específicas de um órgão ou tecido sob investigação, que são produzidas ou liberadas proporcionalmente à injúria ou doença intercorrente. Na nefrologia a análise dos biomarcadores renais permitiu melhor entendimento das doenças, diagnóstico precoce, além de respostas à terapia. Porém, na veterinária ainda existem muitas lacunas não respondidas ou com resultados questionáveis. O objetivo desta revisão de literatura foi abordar temas referentes aos principais biomarcadores renais visando sua realização técnica e aplicabilidade clínica. Biomarcadores, como a cistina C, a lipocalina associada com gelatinase de neutrófilos (NGAL), N-acetil-β-d-glucosaminidase (NAG), a gama glumatiltransferase urinária (GGT urinária), interleucina 18 (IL-18) e a dimetilarginina simétrica (SDMA) serão abordadas nessa revisão. Conclui-se que apesar dos biomarcadores renais serem uma ferramenta promissora para diagnóstico precoce, prognóstico e auxiliar no tratamento de nefropatias são ainda necessários maiores estudos na medicina veterinária buscando identificar suas particularidades clínicas.Palavras-chave: Doença renal crônica, lesão renal aguda, diagnóstico precoce.TBiomarkers are specific substances of an organ or tissue under investigation that are produced or released proportionally to the injury or disease. In nephrology the renal biomarkers allowed a better understanding of the disease, early diagnosis, and response to the therapy. However, there are still many unanswered or questionable gaps in veterinary medicine. The objective of this literature review was to discuss topics related to the renal biomarkers and their technical realization and clinical applicability. New biomarkers, such as cystine C, lipocalin associated with neutrophil gelatinase (NGAL), N-acetyl-β-d-glucosaminidase (NAG), urinary glutathyltransferase (urinary GGT), interleukin 18 (IL-18) and Symmetrical dimethylarginine (SDMA) will be addressed in this review. It is concluded that although renal biomarkers are a promising tool for early diagnosis, prognosis and help in the treatment of nephropathies, further studies in veterinary medicine are still necessary to identify their clinical characteristics and relevance.

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“…In veterinary clinical practice, serum urea and Cre concentrations are widely used as endogenous markers for evaluating RF in dogs and cats because they are easy and inexpensive to perform [15]. Serum Urea is more likely to increase due to pre-renal factors than serum Cre, whereas both parameters are equally likely to increase due to renal diseases [16].…”

Section: Introductionmentioning

confidence: 99%

Assessment Of Renal Function Using Canine Cystatin-C Levels In Canine Babesiosis And Ehrlichiosis

Pekmezci

Kerem²,

Aysul³

et al. 2015

Acta Veterinaria

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The purpose of the present study was to evaluate serum canine Cystatin-C (Cys-C) concentration for the prediction of renal function (RF) in dogs naturally infected with Babesia canis vogeli and Ehrlichia canis. A total of 46 dogs were enrolled into three groups. Group B included 16 dogs naturally infected with B. c. vogeli, group E 10 dogs naturally infected with E. canis [dogs diagnosed as canine monocytic ehrlichiosis (CME)], and group H involved 20 healthy controls (negative for B. c. vogeli and E. canis). Solely dogs presenting uncomplicated babesiosis caused by B. c. vogeli were enrolled. Serum urea and serum creatinine (Cre) levels with serum Urea:Cre ratios were analyzed. Canine serum Cys-C was determined with a species-specific commercially available and validated ELISA assay as a reference. Mean serum Cys-C levels were 5.28 mg/L, 3.02 mg/L, and 2.30 mg/L for groups B, E and H, respectively. RF in uncomplicated CB caused by B. c. vogeli seems to be affected based on serum Cys-C concentrations. To the author's knowledge, this is the first study reporting that mean serum canine Cys-C levels in dogs with CME demonstrated no elevation. Serum canine Cys-C as a novel RF marker could be used for early detection of renal injury in both babesiosis and CME.

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Evaluation of the Measurement of Serum Cystatin C by an Enzyme-Linked Immunosorbent Assay for Humans as a Marker of the Glomerular Filtration Rate in Dogs

Cited by 42 publications

References 27 publications

Rapid Detection of Cat Cystatin C (cCys-C) Using Immuno-Pillar Chips

Rapid Detection of Cat Cystatin C (cCys-C) Using Immuno-Pillar Chips

Conceitos E Aplicabilidade Dos Principais Biomarcadores Na Nefrologia Veterinária – Revisão De Literatura

Assessment Of Renal Function Using Canine Cystatin-C Levels In Canine Babesiosis And Ehrlichiosis

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