Rapid Detection of Cat Cystatin C (cCys-C) Using Immuno-Pillar Chips

Abstract

We demonstrated a rapid immunoassay for detection of cat cystatin C (cCys-C) which is an important marker for chronic kidney disease in cats, using immuno-pillar chips. The required amount of reagent solution is 200 times smaller than that for the conventional ELISA in the 96-well microplate (0.5 mu L versus 100 mu L). In addition, the total assay time in the proposed method is more than 12 times shorter than in the conventional method (20 min versus 240 min). The limit of detection in the new method of 3 ng mL(-1) is comparable to that of the conventional method (1 ng mL(-1)) and it is in the clinically relevant range

Rapid Enzyme-linked Immunosorbent Assays for Diagnosis of Diabetes in a Compact Disc-shaped Microfluidic Device

“…Therefore, in order to enable rapid detection, several research groups have developed ELISA systems that apply microfluidic technology. [5][6][7] These microfluidic systems have some advantages, such as reducing the amount of reagent, shortening the reaction time, and miniaturizing the apparatus. Especially, some compact disc (CD)-shaped microfluidic devices aiming for field diagnosis at point-of-care-testing (POCT) have been developed, because parallel reactions by a single motor and an automated system can be realized.…”