Microfluidic electrochemical immunosensor for the determination of cystatin C in human serum

Devi, K. S. Shalini; Krishnan, Uma Maheswari

doi:10.1007/s00604-020-04503-4

Cited by 27 publications

(10 citation statements)

References 31 publications

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“…After Mucin1 was bound to the immunoaffinity layer, the signal was measured using DPV, and the LOD and linear range were calculated to be 0.04 U/mL and 0.1-50 U/mL, respectively. Devi et al developed a microfluidic CysC immunosensor based on a CS-GO nanocomposite [45]. The electrode was modified using a nanocomposite and the antibodies against CysC were immobilized using EDC/NHS chemistry.…”

Section: Chemical Immobilization Of Antibodiesmentioning

confidence: 99%

Recent Progress in Electrochemical Immunosensors

Kim

Park

2021

Biosensors

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Biosensors used for medical diagnosis work by analyzing physiological fluids. Antibodies have been frequently used as molecular recognition molecules for the specific binding of target analytes from complex biological solutions. Electrochemistry has been introduced for the measurement of quantitative signals from transducer-bound analytes for many reasons, including good sensitivity. Recently, numerous electrochemical immunosensors have been developed and various strategies have been proposed to detect biomarkers. In this paper, the recent progress in electrochemical immunosensors is reviewed. In particular, we focused on the immobilization methods using antibodies for voltammetric, amperometric, impedimetric, and electrochemiluminescent immunosensors.

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Section: Chemical Immobilization Of Antibodiesmentioning

confidence: 99%

Recent Progress in Electrochemical Immunosensors

Kim

Park

2021

Biosensors

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“…Compared with the modified GCE, this paper-based sensor shows a LOD of 2.6 pg mL À1 with a sensitivity of around 15.5 mA ng À1 . 133 Yang and his team with the concept of a laser-engraved (LEG) wearable bio-sensor that could be mass-produced to detect tyrosine levels (TYR) and uric acid (UA). A chemical sensor (LEG-CS) over polyimide for measuring low-level TYR and UA, a physical sensor (LEG-PS) for temperature and respiration monitoring and a multi-inlet micro-fluidic module for dynamic sweat sampling are all included in this device.…”

Section: God(fad) + Glucose God (Fadhmentioning

confidence: 99%

“…over a wax-coated paper following screen printing of Ag/AgCl paste. Compared with the modified GCE, this paper-based sensor shows a LOD of 2.6 pg/μL with a sensitivity of around 15.5 μA/ng133 . Yang and his team with the concept of a laser-engraved (LEG) wearable bio-sensor that could be mass-produced to detect tyrosine levels (TYR) and uric acid (UA).…”

mentioning

confidence: 98%

Microfluidic sensors based on two-dimensional materials for chemical and biological assessments

2022

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“…In the eye, CysC is secreted by the retinal pigment epithelium and could contribute to the progression of macular degeneration (Zurdel et al., 2002; Paraoan et al., 2010), thus explaining the correlation between serum CysC and DR. Notably, CysC has been analysed in serum using an electrochemical immunosensor with high sensitivity (Devi & Krishnan, 2020). Using 1.2 mg/L as a cut‐off value the device showed 85% accuracy in predicting DR in a small ( n = 10) cohort of clinical samples.…”

Section: Markersmentioning

confidence: 99%

Diagnostic circulating biomarkers to detect vision‐threatening diabetic retinopathy: Potential screening tool of the future?

Frudd

Sivaprasad

Raman

et al. 2021

Acta Ophthalmologica

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With the increasing prevalence of diabetes in developing and developed countries, the socio‐economic burden of diabetic retinopathy (DR), the leading complication of diabetes, is growing. Diabetic retinopathy (DR) is currently one of the leading causes of blindness in working‐age adults worldwide. Robust methodologies exist to detect and monitor DR; however, these rely on specialist imaging techniques and qualified practitioners. This makes detecting and monitoring DR expensive and time‐consuming, which is particularly problematic in developing countries where many patients will be remote and have little contact with specialist medical centres. Diabetic retinopathy (DR) is largely asymptomatic until late in the pathology. Therefore, early identification and stratification of vision‐threatening DR (VTDR) is highly desirable and will ameliorate the global impact of this disease. A simple, reliable and more cost‐effective test would greatly assist in decreasing the burden of DR around the world. Here, we evaluate and review data on circulating protein biomarkers, which have been verified in the context of DR. We also discuss the challenges and developments necessary to translate these promising data into clinically useful assays, to detect VTDR, and their potential integration into simple point‐of‐care testing devices.

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Microfluidic electrochemical immunosensor for the determination of cystatin C in human serum

Cited by 27 publications

References 31 publications

Recent Progress in Electrochemical Immunosensors

Recent Progress in Electrochemical Immunosensors

Microfluidic sensors based on two-dimensional materials for chemical and biological assessments

Diagnostic circulating biomarkers to detect vision‐threatening diabetic retinopathy: Potential screening tool of the future?

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