Assessment Of Renal Function Using Canine Cystatin-C Levels In Canine Babesiosis And Ehrlichiosis

Abstract

The purpose of the present study was to evaluate serum canine Cystatin-C (Cys-C) concentration for the prediction of renal function (RF) in dogs naturally infected with Babesia canis vogeli and Ehrlichia canis. A total of 46 dogs were enrolled into three groups. Group B included 16 dogs naturally infected with B. c. vogeli, group E 10 dogs naturally infected with E. canis [dogs diagnosed as canine monocytic ehrlichiosis (CME)], and group H involved 20 healthy controls (negative for B. c. vogeli and E. canis). Solely dogs presenting uncomplicated babesiosis caused by B. c. vogeli were enrolled. Serum urea and serum creatinine (Cre) levels with serum Urea:Cre ratios were analyzed. Canine serum Cys-C was determined with a species-specific commercially available and validated ELISA assay as a reference. Mean serum Cys-C levels were 5.28 mg/L, 3.02 mg/L, and 2.30 mg/L for groups B, E and H, respectively. RF in uncomplicated CB caused by B. c. vogeli seems to be affected based on serum Cys-C concentrations. To the author's knowledge, this is the first study reporting that mean serum canine Cys-C levels in dogs with CME demonstrated no elevation. Serum canine Cys-C as a novel RF marker could be used for early detection of renal injury in both babesiosis and CME.

Cistatina C sérica em cães criticamente enfermos em UTI

“…Considerando-se isso, discretas alterações devem ser valorizadas, pois podem representar mudanças significativas no quadro clínico e exercerem influência no prognóstico, da mesma forma que Neri (2007) e Grubb (2011) mencionaram em seus estudos. A precocidade e a correlação entre a concentração sérica de cistatina C e TFG foram demonstradas anteriormente em cães com disfunção renal (Jensen et al 2001, Braun et al 2002, Wehner et al 2008, Miyagawa et al 2009, Pasa et al 2009, Didem et al 2015. Kavitha et al (2011) ao avaliarem cães com suspeita de lesão renal observaram aumento da cistatina C em 35%, e corroboraram sua superioridade comparada à creatinina, dados semelhantes foram encontrados por Choi et al (2017) ao avaliarem cães cardiopatas.…”

Clinical Role, Diagnostic and Prognostic Value of Human Cystatin C Through the Body

“…Serum Cystatin C is a relatively new endogenous marker that offers the advantages that its produced at a constant rate by all nucleated cells, it is freely filtered by the renal glomerulus, and entirely catabolized in the proximal tubule 19 20 21 22 . Its filtration is unchanged in kidney tubular diseases where its urinary excretion is increased 20 . The multi-ligand endocytic receptors megalin and cubilin are heavily expressed in the proximal tubule brush border membrane and essential for the reabsorption of a large number of filtered proteins in the proximal tubule 23 .…”