Evaluation of salivary total oxidant-antioxidant status and DNA damage of children undergoing fixed orthodontic therapy

Güler, Çiğdem; Toy, Ebubekir; Öztürk, Feral; Güneş, Dilek; Karabulut, Aysun Bay; Otlu, Önder

doi:10.2319/110113-798.1

Cited by 15 publications

(10 citation statements)

References 29 publications

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“…However, this decrease in cell viability was not statistically significant. A similar result was found in studies by Guler et al, [22] wherein no cytotoxic effect was seen in children undergoing orthodontic treatment during 3 months of the evaluation period. However, a study by Faccioni et al [13] and Hafez et al [21] showed decreased cellular viability among patients with orthodontic appliances as compared to the control group.…”

Section: Discussionsupporting

confidence: 89%

In vivo Study on Release of Nickel, Chromium, and Zinc and DNA Damage in Buccal Mucosa Cells from Patients Treated with Fixed Orthodontic Appliances

Quadras¹,

Nayak

Kumari³

et al. 2018

J Indian Orthod Soc

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Background: Fixed orthodontic appliances release metal ions such as nickel (Ni), chromium (Cr), and zinc (Zn), which can initiate DNA damage in oral mucosa cells. Aims and Objectives: Assessment of metal ion levels and possible DNA damage in buccal mucosa cells in patients undergoing fixed orthodontic treatment. Materials and Methods: The present study was undertaken on fifty participants in the age range of 15-40 years visiting the department of orthodontics and dentofacial orthopedics, ABSMIDS, selected after applying inclusion and exclusion criteria. Buccal mucosa cells were scraped using interdental brush and subjected to atomic absorption spectrometry and comet assay to assess the amount of metal ions and DNA damage, respectively, before insertion and at 6 months, 1 year, and 1.5 years after insertion of appliance. Data were analyzed using SPSS software version 17. The mean metal ion levels and cell damage before and after treatment were compared using paired t-test. P < 0.05 was considered statistically significant. Results: Ni ions increased to 62.85 ± 4.81 ppb at the end of 1.5 years from 22 ± 2.3 ppb at baseline before the insertion of appliance, Cr ion levels reached 58.3 ± 5.1 ppb from 20 ± 3.8 ppb, and Zn ion levels reached 562.8 ± 66.5 ppb from 197.4 ± 68.5 ppb, respectively. This result was significant with P < 0.01. A decrease in cell viability and an increase in head diameter, % DNA in tail, and tail length were found at the end of 1.5 years as compared to baseline with P < 0.05. Conclusion: Fixed orthodontic appliances showed an increased metal ion levels and DNA damage to a certain extent which has no clinical impact on buccal mucosa cells in patients with fixed orthodontic appliances.

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Section: Discussionsupporting

confidence: 89%

In vivo Study on Release of Nickel, Chromium, and Zinc and DNA Damage in Buccal Mucosa Cells from Patients Treated with Fixed Orthodontic Appliances

Quadras¹,

Nayak

Kumari³

et al. 2018

J Indian Orthod Soc

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“…Our results were in agreement with the results obtained by Guler et al, who reported similar findings in their study. 16 In one of the previous study conducted by Gonçalves et al, authors assessed the cytotoxic and genotoxic effect of non-soldered bands (NSB) and silver soldered bands (SSB) on the HepG 2 and HOK cell lines. They observed higher cytotoxicity and genotoxicity in SSB eluates in comparison to NSB samples.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic and Genotoxic effect on the Buccal Mucosa Cells of Patients Undergoing Fixed Orthodontic Treatment

Kapadia¹,

Agarwal

Mishra

et al. 2018

The Journal of Contemporary Dental Practice

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Aim:To evaluate the presence of metal ions and deoxyribonucleic acid damage on the cells of buccal mucosa in subjects scheduled to undergo fixed orthodontic treatment. Materials and methods:Eighty patients scheduled to undergo orthodontic treatment were included in the present study. Samples were collected from buccal mucosa of the subjects at five different intervals: before the starting of the fixed appliance therapy, 5 months after the insertion of the appliance, 10 months after insertion of the appliance, 15 months after insertion of the appliance and 20 months after insertion of the appliance. Flow cytometry was further used for assessment of apoptosis. Comet assay was used for evaluating the metal ions associated deoxyribonucleic acid ((DNA) damage of buccal epithelial cells. Atomic absorption spectrometry was used for measuring the nickel (Ni), chromium (Cr) and zinc (Zn) levels in the cells of the buccal mucosa. Analysis of data was done by SPSS software version 16.0.Results: A significant increase in the Ni, Cr and Zn concentration during orthodontic treatment was observed. A progressive non-significant decrease in the percentage of viable cells from a baseline value to the end of the treatment was observed. A significant increase in the head diameter, DNA in tail and tail length, starting from the pretreatment value to the end of orthodontic treatment, was also observed. Conclusion:Timely checking of deoxyribonucleic acid (DNA) damage and nuclear changes should be done for detecting earlier adverse changes. Clinical significance:In patients wearing orthodontic appliances, no clinical impact occurs by wearing fixed appliances.

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“…On the other hand, 66.7% of the studies that have investigated the salivary TAC in more serious oral diseases, including recurrent aphthous stomatitis (RAS) (the most common oral ulcerative condition) [ 36 , 88 ], stem cell transplantation- (SCT-) related salivary gland injury due to graft versus host disease (GVHD) [ 92 ], oral mucositis induced by high-dose therapy of melphalan in myeloma patients treated with autologous SCT [ 22 ], cleft lip and palate [ 17 ], oral premalignant lesions (leukoplakia, lichen planus, and erythroplakia) [ 13 – 15 , 23 , 29 , 49 , 83 , 113 , 128 ], and oral cancers [ 14 , 24 ], found decreased levels of salivary TAC in these patients ( Table 1 ). Furthermore, salivary TAC was decreased also in patients with peri-implant disease [ 81 ] and in children undergoing fixed orthodontic therapy [ 54 ].…”

Section: Infection and Oral And Renal Statusmentioning

confidence: 99%

Salivary and Urinary Total Antioxidant Capacity as Biomarkers of Oxidative Stress in Humans

Peluso

Raguzzini

2016

Pathology Research International

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Total Antioxidant Capacity (TAC) is a biomarker often used in order to investigate oxidative stress in many pathological conditions. Saliva and urine can be collected noninvasively and represent attractive diagnostic fluids for detecting biomarkers of various pathological conditions. The reviewed case-control and intervention studies that measured salivary or urinary TAC revealed that diseases, antioxidant foods, or supplements and age, gender, and lifestyle factors influenced salivary or urinary TAC. Salivary and urinary TAC were particularly affected by oral or renal status, respectively, as well as by infection; therefore these factors must be taken into account in both case-control and intervention studies. Furthermore, some considerations on sample collection and normalization strategies could be made. In particular, unstimulated saliva could be the better approach to measure salivary TAC, whereas 24 h or spontaneous urine collection should be chosen on the basis of the study outcome and of the creatinine clearance. Finally, the uric acid-independent TAC could be the better approach to evaluate red-ox status of body, in particular after nutritional interventions and in diseases associated with hyperuricaemia.

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Evaluation of salivary total oxidant-antioxidant status and DNA damage of children undergoing fixed orthodontic therapy

Abstract: Fixed orthodontic appliances bonded with the tested composites did not increase the cytotoxicity markers in saliva.

Cited by 15 publications

References 29 publications

In vivo Study on Release of Nickel, Chromium, and Zinc and DNA Damage in Buccal Mucosa Cells from Patients Treated with Fixed Orthodontic Appliances

In vivo Study on Release of Nickel, Chromium, and Zinc and DNA Damage in Buccal Mucosa Cells from Patients Treated with Fixed Orthodontic Appliances

Cytotoxic and Genotoxic effect on the Buccal Mucosa Cells of Patients Undergoing Fixed Orthodontic Treatment

Salivary and Urinary Total Antioxidant Capacity as Biomarkers of Oxidative Stress in Humans

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