Abstract:Aim:To evaluate the presence of metal ions and deoxyribonucleic acid damage on the cells of buccal mucosa in subjects scheduled to undergo fixed orthodontic treatment.
Materials and methods:Eighty patients scheduled to undergo orthodontic treatment were included in the present study. Samples were collected from buccal mucosa of the subjects at five different intervals: before the starting of the fixed appliance therapy, 5 months after the insertion of the appliance, 10 months after insertion of the appliance, … Show more
“…For example, Faccioni et al demonstrated DNA damage in oral mucosa cells in patients undergoing orthodontic treatment (14,15). Others also detected DNA damage in oral mucosal cells as a result of metals released by orthodontic devices in the oral cavity (16,17). However, Westhphalen et al were not able to detect genetic damage in oral mucosal cells following orthodontic therapy (18).…”
Background/Aim: Genotoxicity is the capacity of an agent to induce damage to DNA. Given the close relationship between genotoxicity and carcinogenesis, several assays have been developed for detecting genetic damage. Among them, the single-cell gel (comet) assay plays an important role for evaluating DNA damage in mammalian cells, including those of the oral cavity. The purpose of this article was to provide a critical review of the application of single-cell gel comet assay to buccal cells. Material and Methods: A search of the scientific literature was conducted of published studies available on single-cell gel comet assay and oral cells. Results: The results showed that the majority of studies were conducted on humans, whereas few were designed for use in rodents and in vitro. Conclusion: Further studies within the field are relevant for better understanding the underlying mechanisms of genotoxicity in oral cells, especially since the use of humans is quite complicated due to issues of ethics.
“…For example, Faccioni et al demonstrated DNA damage in oral mucosa cells in patients undergoing orthodontic treatment (14,15). Others also detected DNA damage in oral mucosal cells as a result of metals released by orthodontic devices in the oral cavity (16,17). However, Westhphalen et al were not able to detect genetic damage in oral mucosal cells following orthodontic therapy (18).…”
Background/Aim: Genotoxicity is the capacity of an agent to induce damage to DNA. Given the close relationship between genotoxicity and carcinogenesis, several assays have been developed for detecting genetic damage. Among them, the single-cell gel (comet) assay plays an important role for evaluating DNA damage in mammalian cells, including those of the oral cavity. The purpose of this article was to provide a critical review of the application of single-cell gel comet assay to buccal cells. Material and Methods: A search of the scientific literature was conducted of published studies available on single-cell gel comet assay and oral cells. Results: The results showed that the majority of studies were conducted on humans, whereas few were designed for use in rodents and in vitro. Conclusion: Further studies within the field are relevant for better understanding the underlying mechanisms of genotoxicity in oral cells, especially since the use of humans is quite complicated due to issues of ethics.
“…Due to this long contact time, conventional braces can cause changes in DNA. A study by Kapadia et al [38] showed that due to the content of metal ions, fixed orthodontic appliances reduce cell viability by altering DNA and promoting the process of apoptosis. Another category of orthodontic appliances is polycarbonate-based orthodontic brackets.…”
Malocclusion and teething problems are common health problems globally, affecting people of all ages, especially children and adolescents. In addition to the pathophysiological complications associated with orthodontic problems, they also affect the well-being of the individual. Orthodontic appliances are frequently used, even from an early age, and their activity in different biological environments is very varied and incompletely described. Due to these considerations, the purpose of the study was to evaluate the toxicological profile of the biological environment (saliva at three pH values: 3, 7, and 10) of two elastodontic orthodontic appliances: Myobrace (MB) and LM TrainerTM 2 (LMD). In vitro techniques applied were conducted on human keratinocytes to evaluate cell viability (Alamar blue assay) and gene expression real-time reverse transcription–polymerase chain reaction (RT-PCR technique). In addition, it was assessed the irritating effect on the vascular plexus using as a biological model the chorioallantoic membrane of the hen’s egg by applying the hen’s egg-chorioallantoic membrane (HET-CAM) method. The obtained results showed a decrease in cell viability up to 82% in the case of LMD at pH = 3, a slight increase in mRNA expression for the anti-apoptotic marker (Bcl-2 and Bcl-xL), and a decrease in mRNA expression for the pro-apoptotic marker (Bad), and any type of toxic change at the capillary level (irritation score being below 0.9). Based on the data obtained, it can be stated that MB and LMD biological environments, at different pH values, present a safe toxicological profile.
“…[ 3 4 ] Orthodontic appliances may release amounts of metal ions;[ 5 6 7 8 9 10 ] this can lead to diverse toxic effects such as DNA damages and oral lesions. [ 11 12 ] Some studies have already shown the release of metal ions into saliva and the cytotoxicity and genotoxicity of silver solder in oral cells. [ 13 14 15 16 17 ] In addition to the metallic elements of stainless steel, such as nickel (Ni), chromium (Cr), and iron (Fe), silver solder alloys contain silver (Ag), copper (Cu), and zinc (Zn).…”
OBJECTIVE:
To evaluate the surface roughness and ion release of silver-soldered joints by using two polishing methods.
METHODS:
174 orthodontic bands with and without silver-soldered joints were evaluated and divided into three groups: two experimental, with different polishing methods (SP1 and SP2), and one control (SS) composed of bands without silver solder. For ionic release, 50 bands of each group were immersed in saline solution and submitted to atomic absorption spectrophotometry to quantify the amount of Fe, Ni, Cr (in all the three groups), Ag, Cu, Cd, and Zn (in the two experimental groups). A rugosimeter was employed to verify the surface roughness.
RESULTS:
Ni and Cr were released in higher amounts after soldering. Cd, Ag, Zn, and Cu may be released from silver-soldered bands independently of the polishing method employed. Ag was released in higher amounts from the soldered bands that presented higher surface roughness.
CONCLUSIONS:
Differences exist in relation to the surface roughness of silver-soldered bands when distinct polishing methods are used. Toxic ions may be released from silver soldered joints and higher surface roughness may cause higher ionic release.
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