The Use of Single-Cell Comet Assay on Oral Cells: A Critical Review

Souza, Ana Carolina Flygare; Yujra, Veronica Quispe; Pisani, Luciana Pellegrini; Viana, Milena de Barros; Castro, Gláucia Monteiro de; Ribeiro, Daniel Araki

doi:10.21873/anticanres.13556

Cited by 5 publications

(1 citation statement)

References 32 publications

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“…Migrating DNA helixes appear like 'comets'. The size and shape of the comet and the distribution of DNA within the comet are associated with the extent of DNA damage [13].…”

Section: Materyalmentioning

confidence: 99%

Bilgisayarlı Tomografi İle Yapılan Perkütan Torasik Kitle Biyopsilerinde Lenfositlerdeki DNA Hasarının Değerlendirilmesi

Ayyildiz

ASLANKOÇ

Saygın

et al. 2023

Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi

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Purpose: The increase in the medical and industrial uses of radiation in the last century has caused people to be exposed to higher doses of radiation. Although the harms of high-dose radiation on human health are known, the effects of low-dose radiation on health have not yet been fully elucidated. In this study, we aimed to investigate DNA damage in lymphocytes in patients with lung, pleura/thoracic wall masses planned for percutaneous thoracic mass biopsy with computed tomography (CT). Methods: Sixteen patients referred to the Radiology Clinic of a public institution hospital with lung, pleura/thoracic wall masses and scheduled to undergo a CT-guided percutaneous biopsy were included in the study. All the biopsies were performed with a 128-slice CT device (Definition AS, Siemens Medical Solutions, Forchheim, Germany). Lymphocytes were analyzed using the comet assay in the venous blood samples taken from the patients before and after the biopsy procedure. DNA damage was quantitatively evaluated with the imaging analysis method. Results: In the CT analysis data of the study group, the mean scan distance was found to be 19.92±3.60 sec, the mean total milliampere-seconds was 807.43±304.51, and the mean dose-length product was 765.44±278.36 mGy.cm. The mean comet score was 200.50±40.54 for the cells that migrated before the procedure and 237.37±27.85 for those migrating after the procedure. The post-procedure comet scores significantly increased compared to the pre-procedure comet scores (p=0.038). Conclusion: Post-procedure DNA damage was detected in patients who underwent CT-guided percutaneous biopsy.

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“…Migrating DNA helixes appear like 'comets'. The size and shape of the comet and the distribution of DNA within the comet are associated with the extent of DNA damage [13].…”

Section: Materyalmentioning

confidence: 99%