Evaluation of Popular Stains for the Diagnosis of American Cutaneous Leishmaniasis

Castillo, Carmen Manuela; Rojas, Carlos

doi:10.1590/s0074-02761997000400016

Cited by 5 publications

(4 citation statements)

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“…The Field's staining method was used in this study following the protocol of Sivanandam and Mak (1974) with a slight modification of timing where the original 10 to 12 s for malarial staining was extended to 15 to 20 s in the study by Afzan et al, (2010) to optimize staining results for detecting T. gallinae. Castillo and Rojas (1997) reported that Field's stain was more sensitive than Giemsa (78.7% versus70.5%), to diagnosis of American cutaneous leishmaniasis. While Afzan et al, (2010) reported that both Giemsa and Field's stain are equally good for detecting T. vaginalis.…”

Section: Resultsmentioning

confidence: 99%

Isolation, Diagnosis and Cultivation of Trichomonas gallinae from Domestic Pigeon in Kirkuk City, Iraq

Hamad¹,

Hassan²

2017

Int.J.Curr.Res.Aca.Rev.

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Article InfoThis research was conducted to study the spread of an important unicellular protozoan Trichomonas gallinae isolated from the mouth and esophagus of three groups of domestic pigeon Colomba livia domestica by wet mount preparation. A total of 609 domestic pigeons were tested in Kirkuk city, Iraq from August 2015 to April 2016, the total infectious rate of the prevalence were 49.26%, distributed to 64.92%, 50.98% and 30.41% in Alzajel, pakistani and zubaeri pigeon respectively. The present study compared three different stains to characterize the shape of T. gallinae namely Giemz, Gram and for first time Field's stain, the Giemza stain showed efficient to stain T. gallinae more than other two stains, although Field's stain requires less time for staining compared to Giemza and Gram's stain. The parasites are cultivated in three prepared culture media (In-tube culture system), which are modified Diamond media (TYM), Cysteine Peptone Maltose media (CPLM) and Trichomonas CM0161 media (CM 161) with the initial inoculation of 2X105 cell/ml, the fourth media was In-pouch culture system that is commercially available with the initial inoculation of 105 cell/ml. In-pouch TV culture media is used for the first time to diagnose and to grow T. gallinae which is a special media for diagnosis of the vaginal trichomoniasis caused by Trichomonas vaginalis. The result showed the best growth of T. gallinae in In-pouch media compared with In-tube culture system, modified diamond media and CM 161 gave the high growth to 48 hours while CPLM gave the high growth from 24 hours, the CM 161 media showed acute decrease in the number of T. gallinae after 48 hour.

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Section: Resultsmentioning

confidence: 99%

Isolation, Diagnosis and Cultivation of Trichomonas gallinae from Domestic Pigeon in Kirkuk City, Iraq

Hamad¹,

Hassan²

2017

Int.J.Curr.Res.Aca.Rev.

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“…On the other hand, histopathology allows one to identify other diseases that show clinical features similar to those of leishmaniasis (14,20). Touch preparations in general give a fair degree of positivity and are less expensive methods, but they can be time-consuming (3).…”

Section: Discussionmentioning

confidence: 99%

Use of PCR in Diagnosis of Human American Tegumentary Leishmaniasis in Rio de Janeiro, Brazil

Pirmez¹,

Trajano²,

Neto

et al. 1999

J Clin Microbiol

102

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In Brazil, the most common etiological agent of American tegumentary leishmaniasis is Leishmania(Viannia) braziliensis. In general, diagnostic techniques envisage the visualization of the parasite, but that technique has a low sensitivity. The main purpose of the present work was to evaluate the PCR as a routine tool for the diagnosis of leishmaniasis. Biopsy specimens from cutaneous or mucosal lesions were taken from 230 individuals from areas where Leishmania is endemic: 216 patients who had a clinical picture suggestive ofl leishmaniasis and 14 individuals with cutaneous lesions due to other causes. Each specimen was processed for histopathologic examination, culture, touch preparation, and DNA isolation. Oligonucleotides that amplify the conserved region of the minicircle molecules ofLeishmania were used in a hot-start PCR. While at least one conventional technique was positive for Leishmania for 62% (134 of 216) of the patients, PCR coupled to hybridization was positive for 94% (203 of 216) of the patients. The 14 patients whose clinical picture was not suggestive of leishmaniasis had negative results by all techniques. The impact of the PCR was striking in mucosal disease. While the disease in only 17% (4 of 24) of the patients could be diagnosed by conventional techniques, PCR was positive for 71% (17 of 24) of the patients. Hybridization showed that all cases of disease were caused by parasites belonging to the Viannia subgenus. Altogether, the results indicate that PCR is a valuable tool for the diagnosis of leishmaniasis on a routine basis and is likely to provide valuable epidemiological information about the disease in countries where it is endemic.

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“…Giemsa staining has largely replaced H&E as the diagnostic standard, whereby amastigotes show an intense blue staining pattern 5 . In areas where Giemsa use is limited, such as the endemic region of Colombia, a rapid field stain may serve as a fast and cheap alternative 6 …”

Section: Discussionmentioning

confidence: 99%

“…5 In areas where Giemsa use is limited, such as the endemic region of Colombia, a rapid field stain may serve as a fast and cheap alternative. 6 Even with H&E and Giemsa stains, CL may show parasites on histopathology in only 67% of patients. 7 Because of the presence of non-caseating granulomas, cases may also be mistaken for sarcoidosis or other granulomatous processes when amastigotes are scarce or absent in later stages of disease.…”

Section: Discussionmentioning

confidence: 99%

Diffuse cutaneous leishmaniasis and HIV co‐infection: A case report and review of the literature

et al. 2021

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Leishmaniasis is a parasitic infection classified into Old World and New World species of sandfly vectors, classically Phlebotomus and Lutzomyia, respectively. 1 Old World leishmaniasis, seen in the Eastern hemisphere, typically manifests as cutaneous and visceral disease. New World leishmaniasis, arising in Latin and South America, results in cutaneous, mucocutaneous, and visceral disease. The incidence of

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Evaluation of Popular Stains for the Diagnosis of American Cutaneous Leishmaniasis

Cited by 5 publications

References 0 publications

Isolation, Diagnosis and Cultivation of Trichomonas gallinae from Domestic Pigeon in Kirkuk City, Iraq

Isolation, Diagnosis and Cultivation of Trichomonas gallinae from Domestic Pigeon in Kirkuk City, Iraq

Use of PCR in Diagnosis of Human American Tegumentary Leishmaniasis in Rio de Janeiro, Brazil

Diffuse cutaneous leishmaniasis and HIV co‐infection: A case report and review of the literature

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