1999
DOI: 10.1128/jcm.37.6.1819-1823.1999
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Use of PCR in Diagnosis of Human American Tegumentary Leishmaniasis in Rio de Janeiro, Brazil

Abstract: In Brazil, the most common etiological agent of American tegumentary leishmaniasis is Leishmania(Viannia) braziliensis. In general, diagnostic techniques envisage the visualization of the parasite, but that technique has a low sensitivity. The main purpose of the present work was to evaluate the PCR as a routine tool for the diagnosis of leishmaniasis. Biopsy specimens from cutaneous or mucosal lesions were taken from 230 individuals from areas where Leishmania is endemic: 216 patients who had a clinical pictu… Show more

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Cited by 102 publications
(46 citation statements)
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“…Furthermore, sensitivity reports vary from 0.2% for direct examination to 69% when various parasite identification methods are combined (direct examination, biopsy, culture, and histopathology), due to low parasite numbers in tissue. [1][2][3][4][5][6] Clinical and histopathologic findings are similar to those observed in other infectious diseases, such as histoplasmosis, leprosy, blastomycosis, syphilis, and tuberculosis. Lymphoma and Wegener's disease are included in the differential diagnosis.…”
Section: Introductionsupporting
confidence: 60%
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“…Furthermore, sensitivity reports vary from 0.2% for direct examination to 69% when various parasite identification methods are combined (direct examination, biopsy, culture, and histopathology), due to low parasite numbers in tissue. [1][2][3][4][5][6] Clinical and histopathologic findings are similar to those observed in other infectious diseases, such as histoplasmosis, leprosy, blastomycosis, syphilis, and tuberculosis. Lymphoma and Wegener's disease are included in the differential diagnosis.…”
Section: Introductionsupporting
confidence: 60%
“…[24][25][26] Ideally, a diagnosis of mucocutaneous leishmaniasis should be based upon the demonstration of Leishmania 23 Serology may have false positive results due to crossed reactions with other diseases, i.e., trypanosomiases, malaria, erythematous lupus, and toxoplasmosis. 5,30 On clinical practice, the diagnosis is made using clinical, histopathologic, and laboratory criteria. We used a composite reference standard that included these criteria, which we used in our previous study with satisfactory results.…”
Section: Discussionmentioning
confidence: 99%
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“…Using PCR, genetic investigations can be performed with a broad range of DNA sources, such as amber-conserved insects, mummies, slides, blood or semen [8]. A number of reports have described the use of PCR for amplifying DNA from Plasmodium [9,10], Mycobacterium tuberculosis [11], Cryptosporidium parvum [12] and Leishmania [5][6][7][13][14][15][16][17]. The purpose of the present study was to investigate whether extraction and amplification of Leishmania DNA could be achieved from tissue lesion imprints on Giemsa-stained slides that had been stored for up to 36 years.…”
mentioning
confidence: 99%