Evaluation of microvessel density in central and peripheral giant cell granulomas

Hallikeri, Kaverı; Acharya, Swetha; Koneru, Anila; Trivedi, Dhiraj J.

doi:10.15713/ins.jcri.36

Cited by 6 publications

(9 citation statements)

References 20 publications

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“…Our results are in accordance with the results of previous studies as that of Hallikeri et al, 2015 andSouza et al, 2000 in which the expression of Ki-67 was mainly restricted to mononuclear cells while few giant cells showed positive reactivity. They suggested that mononuclear cells are the proliferative components of PGCG 10,49 .…”

Section: Discussionsupporting

confidence: 93%

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Evaluation of Development and Growth of Peripheral Giant Cell Granuloma Using Osteocalcin, Cd68, Cd34, and Ki-67 Markers

Kamal

Behairy

2017

Egyptian Dental Journal

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Peripheral giant cell granuloma of jaws is considered as a reactive lesion. This lesion is characterized by the presence of multiple multinucleated giant cells (MNGCs) in addition to mononuclear cells. The origin of the multinucleated giant cells is controversial.Objective: Assessment of the expression of CD34, CD68 and osteocalcin in peripheral giant cell granulomas to clarify the origin of MNGCs and to determine Ki-67 positive cells which are responsible for the growth of the lesion. Materials and Methods:In this study, nineteen cases of peripheral giant cell granuloma presented in Oral and Maxillofacial Surgery Department and were treated by surgical excision.Five sections were prepared from a paraffin-embedded specimen of each case and stained with Haematoxylin and Eosin (H&E), CD68, CD34, Ki-67, and osteocalcin.Results: Ki-67, showed positive expression in mononuclear cells and few multinucleated giant cells.CD34 showed negative expression in mononuclear cells and multinucleated cells, while it showed positive results in endothelial cells of the blood vessels. CD 68 showed positive expression in multinucleated cells and few stromal cells. Osteocalcin (OC); bone formation marker showed positive expression in MNGCs and bone trabeculae.Conclusions: These results suggest that multinucleated giant cells are osteoclastic in nature and may derive from monocyte/macrophage lineage and not the endothelial cells. Additionally, we underlined the importance of mononuclear cells in the growth of these lesions while multinucleated cells showed no role in their growth and their presence is considered reactive.

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Section: Discussionsupporting

confidence: 93%

“…To provide appropriate treatment, the pathogenesis of the lesions is very important 10 . However, the origin of giant cell lesions of oral cavity has been subjected to controversy for several years.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Development and Growth of Peripheral Giant Cell Granuloma Using Osteocalcin, Cd68, Cd34, and Ki-67 Markers

Kamal

Behairy

2017

Egyptian Dental Journal

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“…Mean vascular density is commonly used as a critical prognostic marker of neoplasms; therefore our findings indicate that central giant cell granuloma is more aggressive and invasive than AM, OKC and PG (Maeda et al, 1995). In the present study, mean vascular density for Central Giant Cell Lesion was 32.99±0.77, this is in line with the findings of , who used CD34 as a marker of angiogenesis (Hallikeri et al, 2015). Furthermore, macrophages are associated with the angiogenesis in tumor stroma, thereby stimulating and modulating angiogenic process.…”

Section: Discussionsupporting

confidence: 91%

“…Furthermore, macrophages are associated with the angiogenesis in tumor stroma, thereby stimulating and modulating angiogenic process. This is supported by the high presence of macrophages in giant cell lesions with comparative ratios to blood vessels (Hallikeri et al, 2015).…”

Section: Discussionmentioning

confidence: 89%

Assessment of Tumor Angiogenesis by Expression of CD 105 in Ameloblastoma, Odontogenic Keratocyst and Central Giant Cell Lesion

Ali

Khan

Sultana

et al. 2020

Asian Pac J Cancer Prev

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Background: Angiogenesis is critical for tumor growth and reflects the aggressive behavior of invasive odontogenic lesions [like amelogenesis (AM) (AM), Odontogenic Keratocyst (OKC) and Central giant cell lesion (CGCL)]. Mean vascular density (MVD) shows the angiogenic potential and CD105 is an ideal endothelial biomarker due to its specificity to new blood vessels for MVD detection. The aim of the study was to compare the MVD (angiogenic potential) among AM, OKC and CGCL in comparison to Pyogenic Granuloma (PG) using CD105 biomarker. Methods: Sixty-four primary cases of odontogenic invasive tumors (AM, OKC and CGCL) and PG, diagnosed clinically and histologically were included in the study, with 16 samples in each group. Tissue samples of peripheral AM, Peripheral GCL of jaws, malignant AM, and specimen with insufficient tissue were excluded. Tissue sections were embedded, processed and stained using Hematoxylin and Eosin (H and E). Immunohistochemistry was performed using antibodies against CD105, with positive brown cytoplasmic staining in the endothelial cells of neo-vasculature. Distinct countable, positively stained endothelial cell or clusters were evaluated under light microscope for identification of MVD. ANOVA and t-test were applied for statistical analysis of data. Results: Highest MVD was displayed in CGCL (32.99±0.77) and the minimum was observed in OKC (7.21± 0.75) respectively. CGCL showed significantly higher MVD to AM, OKC and PG lesions (p<0.05). AM (8.07± 0.36) and Odontogenic Keratocyst (7.21± 0.75) showed comparable MVD, which was lower than PG (14.7± 0.96) and CGCL vascular density (p < 0.01) respectively. Conclusion: CGCL was most aggressive, with highest MVD among the investigated odontogenic lesions (OKC, AM and PG). The proliferative aggressive behavior of Odontogenic Keratocyst is comparable to AM due to comparable mean vascular density.

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“…On the contrary to the findings of the present study, N Mohtasham et al (13) , Kumar et al (14) ,HJ Sherlin et al (15) and Hallikeri K et al (16) supposed a macrophage origin for MNGCs of CGCG and PGCG lesions .…”

Section: Ghaly Amcontrasting

confidence: 99%

Comparing proliferative activity of Giant Cell Granuloma Lesions with Osteoclastic and Histiocytic derivation using PCNA,TRAP and CD163

2020

Mansoura Journal of Dentistry

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Background: Giant cell granulomas of the jaws are lesions that arise either peripherally in periodontal ligament and mucoperiosteum or centrally in the bone. The aim of this study was to evaluate expression of tartrate-resistant acid phosphatase (TRAP) and CD163 proteins in multinucleated giant cells and mononuclear cells and compare proliferative activity between central giant cell granuloma (CGCG) and peripheral giant cell granuloma (PGCG) by immunohistochemistry of PCNA. Methods: The study was conducted on 40 formalin fixed paraffin embedded tissue blocks (20 CGCG ,20 PGCG). Five micron thick sections were cut from the paraffin blocks; two for hematoxylin and eosin to confirm diagnosis, while three sections were prepared for the immunohistochemical detection of TRAP, CD163 and PCNA by using Avidin-Biotin complex according to the manufacturer's instructures. Results: There was statistically significant difference regarding CD163 between CGCG and PGCG, while there was no statistically significant differences of TRAP and PCNA between CGCG and PGCG. Also, there was high negative correlation between immunoexpression of TRAP and CD163 in CGCG and PGCG. There was no correlation between TRAP and PCNA and between CD163 and PCNA. Conclusions: From the findings of the present study, it can be concluded that the histogenesis of MNGs of CGCG and PGCG was suggested to be of the osteoclastic origin, while there was no differences between proliferative activity between these two lesions.

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Evaluation of microvessel density in central and peripheral giant cell granulomas

Cited by 6 publications

References 20 publications

Evaluation of Development and Growth of Peripheral Giant Cell Granuloma Using Osteocalcin, Cd68, Cd34, and Ki-67 Markers

Evaluation of Development and Growth of Peripheral Giant Cell Granuloma Using Osteocalcin, Cd68, Cd34, and Ki-67 Markers

Assessment of Tumor Angiogenesis by Expression of CD 105 in Ameloblastoma, Odontogenic Keratocyst and Central Giant Cell Lesion

Comparing proliferative activity of Giant Cell Granuloma Lesions with Osteoclastic and Histiocytic derivation using PCNA,TRAP and CD163

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