Evaluation of MecA Gene Occurrence of Gram Positive Bacteria Isolated from Patients with Otitis

Akgül, Ömer; Çetin, Yunus; Bora, Gülhan; Çetin, Yaser; Kaya, M.

doi:10.9734/mrji/2018/45565

Cited by 2 publications

(2 citation statements)

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“…It has been reported that more than $2 billion has been spent over the management of these infections 1 . Approximately 7% cases have been diagnosed with chronic ear infections and the rate is relatively huge with respect to developed countries 2 . Generally, hearing loss is considered as one of the major health related issue despite of the fact that ear infections can lead to lethal outcomes in long run [3][4] .…”

Section: Introductionmentioning

confidence: 99%

Analysis of Sensorineural Hearing Loss by Pure Tone Audiometry in Chronic Suppurative Otitis Media

Khatri

Siddiqui

Rahim

et al. 2021

ashkmdc

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Objective: To determine the frequency of sensorineural hearing loss in patients presenting with Chronic Suppurative Otitis Media.Methods: A total of 121 patients who fulfilled the inclusion criteria and visited the ENT Department of Civil Hospital, Karachi were included in the study. Informed consent was taken after explaining the procedure, risks, and benefits of the study. Bone conduction thresholds were measured at frequencies (500, 1000, 2000, 4000 H2) utilizing diagnostic audiometer model TA 155. Air conduction and bone conduction thresholds >30 decibel and no air-bone gap were considered as SNHL. The opposite ear was masked while obtaining bone conduction results. All the collected data were entered into the proforma.Results: Mean ± SD of age was 35.48 ± 7.24 years. The mean ± SD of bone conduction was 29.42 ± 4.28 db. Out of 121 patients, 85 (70.24%) were male and 36 (29.76%) were female. Sensorineural hearing loss was noted in 20 (16.52%) patients. SNHL is found to be associated with age, gender, duration of symptoms, and level of bone conduction with p <0.05.Conclusion: It is concluded that CSOM is associated with SNHL. Aging can act as a precipitating factor in this pathological process. It is therefore recommended that all ENT surgeons should implement early medical and surgical management of any form of CSOM to prevent SNHL which is irreversible in most patients.

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Section: Introductionmentioning

confidence: 99%

Analysis of Sensorineural Hearing Loss by Pure Tone Audiometry in Chronic Suppurative Otitis Media

Khatri

Siddiqui

Rahim

et al. 2021

ashkmdc

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“…MRSA, as we have come to accept, brought about a tumultuous antibiotic's resistance era in hospital and community sceneries worldwide, owing to its increased intransigency to several βlactam antibiotics engendered by the acquisition mecA gene that codes for the production of modified penicillin binding protein 2a (PBP2a) [3].…”

Section: Introductionmentioning

confidence: 99%

Genetic Profiling of Methicillin-resistant Staphylococcus aureus in An African Hospital by Multiplex-PCR

Adeiza

Onaolapo

Olayinka

2020

Journal of Microbiology and Infectious Diseases

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Objectives: Methicillin resistant Staphylococcus aureus (MRSA) nasal carriage often precedes infection. This work aims to genetically profile MRSA carriage of resistant genes (mecA, blaZ and tetM) and certain virulence genes (nuc, pvl, spa and scn). Methods: Phenotypically confirmed Staphylococcus aureus were Screened for oxacillin resistance and assessed for β-lactamase production. Further confirmation of identity was performed by multiplex PCR of the 16SrRNA, mecA, tetM, pvl, nuc, spa and scn genes. DNA gel fingerprint analysis was carried out and the bands were clustered in a dendrogram. Results: The study analysed 81 Staphylococcus aureus isolates for Methicillin resistance out of which 38 (46.9%) were MRSA. The DNA extraction method was effective for all MRSA isolates evaluated. All phenotypic MRSA isolates (100%) were positive for the S. aureus specific 16SrRNA gene confirming that these isolates were S. aureus strains. The nuc gene was detected in 19/38 (50%) of the MRSA isolates, blaZ gene in 14/38 (36.8%) and tetM in 18/38 (47.4%). Conclusions: The need for improved vigilance to recognize MRSA strains cannot be overemphasized. If MRSA control is not tracked, these strains will increase exponentially.

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Evaluation of MecA Gene Occurrence of Gram Positive Bacteria Isolated from Patients with Otitis

Cited by 2 publications

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Analysis of Sensorineural Hearing Loss by Pure Tone Audiometry in Chronic Suppurative Otitis Media

Analysis of Sensorineural Hearing Loss by Pure Tone Audiometry in Chronic Suppurative Otitis Media

Genetic Profiling of Methicillin-resistant Staphylococcus aureus in An African Hospital by Multiplex-PCR

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