Objective: Today, cosmetics sector is one of the thousands of sectors that are inspected within the scope of protection of people's health, preventive health measures, investments made for health rather than illness, and projects made in the name of raising awareness of people without getting sick. Cosmetics need to be produced and maintained in healthy conditions to minimize unwanted effects. The aim of this study is to evaluate the aerobic bacterial contamination of used cosmetic products and the resistance profile (carbapenem and extended-spectrum beta-lactamase) of isolated bacteria.Material and Method: Five hundred samples were made from used cosmetic products. Microbiological evaluation was performed using culture, biochemical tests, Vitek 2 and polymerase chain reaction (PCR) from the samples.Result and Discussion: In this study, used cosmetic products were collected from 500 cosmetic users. A total of 101 (20.2%) bacteria were isolated from used cosmetic products (n = 500). As a result of identification
Infectious diseases have been one of the most important problems affecting all societies since the existence of mankind. Streptococci, one of the causes of these diseases, can be found in the normal flora of the mouth, nose, throat, skin, digestion and genital system in humans. Streptococci are an important cause of diseases in humans such as meningitis, acute otitis media, rhinosinusitis and community-acquired pneumonia. In this study, various clinical samples belonging to the disease were taken between January and Septe mber 2015. All necessary permission documents and the ethics committee approval numbered 2 and dated 13.11.2014 for clinical investigations were obtained. The collected samples were brought to the Microbiology Laboratory of Van Yüzüncü Yıl University Medical Faculty. Analyzes of determined and identified antibiotic resistance genes of isolated and identified streptococci strains were carried out in the Microbiology laboratory of Yüzüncü Yıl University Faculty of Pharmacy by PCR. The detection of pbp1a for penicillin G antibiotic, pbp2x for cefotaxime antibiotic, and gyrA resistance genes for quinolone antibiotics were targeted. In this study, the primers used in Table-2 were taken as reference. A total of 30 streptococcus strains were isolated and identified from various clinical samples including 8 sputum, 6 urine, 5 BOS, 4 blood, 4 nasopharyngeal swab, 2 ear effusion and 1 ab scess. As a result of the PCR analysis, the resistance of pbp1a was found in 6 of the streptococcal isolates, pbp2x in 8, and resistan ce in the gyrA gene region in 5. In our study, no three resistance genes were found in 22 isolates and the presence of all three (pbp1a, pbp2x and gyrA) resistance genes in 5 isolates was determined. It is expected that the data obtained from this research will contribute to national and international knowledge accumulation.
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