Evaluation of in Vivo Immunomodulatory Activity of Aqueous and Ethanolic Extract of Eulophia Nuda L.

Abstract

Objective: The aim of this study was to evaluate the in vivo immunomodulatory activity of an aqueous and ethanolic extract of dried tubers of Eulophia nuda (ENA and ENE).Methods: Effect of both the extracts was evaluated on delayed-type hypersensitivity (DTH) response, serum antibody response, and cyclophosphamideinduced myelosuppression in Swiss albino mice. Results:The extracts showed stimulation of DTH reaction in mice in response to T-cell dependent antigen by both ENE () and ENA (); significant increase in serum antibody titer at of ENA (768.01) and ENE (768.33) extract at 200 mg/kg, compared to control group (213.33); Combined treatment of ENA+Cytochromes P450 [CYP]-25 mg/kg and ENE+CYP-25mg/kg (50, 100, and 200 mg/kg) doses of ENA and ENE each with 25 mg/kg resulted in restoration of bone marrow activity as compared with CYP treatment alone. Conclusion:Both specific and non-specific immunostimulating properties of the ENE and ENA tubers in in vivo experimental methods suggest its therapeutic usefulness in immunocompromised conditions.

Evaluation of in Vivo Immunomodulatory Activity of Aqueous and Ethanolic Extract of Capparis Moonii

“…Phytohemagglutinin-M (PHA) and fetal bovine serum were obtained from Sigma Aldrich at Saint Louis present in the United States of America. All other chemicals used were of grade use for analytical purposes [16].…”

“…On 17-10 days of study, all the mice except in the control category were administered with CYP (25 mg/kg, intraperitoneal) 1 h after administration of the extract or vehicle. Blood samples were collected on day 20 and total count of cells in blood was estimated utilizing a hematology analyzer from Arcus, Diatron, Wien, Austria [16,19,20].…”