Evaluation of in Vivo Immunomodulatory Activity of Aqueous and Ethanolic Extract of Eulophia Nuda L.

Kanase, Vanita; Patil, Diptesh T

doi:10.22159/ajpcr.2018.v11i12.27051

Cited by 3 publications

(3 citation statements)

References 15 publications

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“…Sera samples recovered each time were used for the determination of host primary and secondary humoral responses using Enzyme Linked Immunosorbent Assay (ELISA) as described in the preceding section. 28 Determination of antibody titre by ELISA The antibody titres elicited against OVA in the treated and control groups were estimated by enzyme linked immunosorbent assay. Flat bottom polystyrene plates were coated with 2 µg of OVA or TT in 100 µl bicarbonate-coating buffer (pH 9.6) and incubated overnight at 4 o C. Unbound OVA or TT was then washed off with ELISA wash buffer (PBS-T; containing 0.05% Tween-20 in 0.15 M phosphate-buffered saline [PBS, pH 7.2]).…”

Section: The Effect Of Oral Agl Supplementation Of Agl On Phagocytic ...mentioning

confidence: 99%

Modulatory Effects of Aqueous Leaf Extract of Gongronema latifolium (asclepiadaceae) on Some Natural and Adaptive Immune Responses in Rodents

2023

TJNPR

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In traditional medicine across various ethnic groups, extracts and decoctions of G. latifolium leaves are utilized for diverse purposes. For instance, the Ikale community in Nigeria reportedly uses it to treat malaria, nausea, and anorexia. 4,5 The Efik and Quas tribes of Cross River State in Nigeria also utilize the leaves for treating malaria, diabetes, hypertension, and constipation. 6 In Senegal and Ghana, G. latifolium leaves are applied on children's joints to aid walking, while the boiled fruits are consumed as a laxative. 7 According to another report, it is use in some West African communities to treat cough, intestinal worms, dysentery, dyspepsia and malaria 7 . Traditionally, asthmatic patients have been reported to get relieved when they chew the fresh leaves of G. latifolium while a cold maceration of the roots of the plant can be consumed as treatment for the asthmatic conditions. 7 My other uses have also been reported, hepatitis, bilharzia infection, microbial infections and dyspepsia. [8][9] They have also been scientific efforts to investigate the pharmacological properties responsible for the claimed benefits of G. latifolium extract and fractions. Antiinflammatory, 3,5,10 antimicrobial, 3,5,11 antidiabetic activity, 6,12 antioxidant, 13-14 anticancer, 15 hepato-and nephroprotective activities 16 are among the many reported pharmacological effects of various extracts. Many medicinal plants have been utilized to treat infections and other diseases due to their different effects on animal immune systems. We have previously shown that various versatile medicinal plants, at least partly, owe their medicinal properties to the modulation of specific and non-specific immune responses to antigenic challenges and

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Section: The Effect Of Oral Agl Supplementation Of Agl On Phagocytic ...mentioning

confidence: 99%

Modulatory Effects of Aqueous Leaf Extract of Gongronema latifolium (asclepiadaceae) on Some Natural and Adaptive Immune Responses in Rodents

2023

TJNPR

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“…Phytohemagglutinin-M (PHA) and fetal bovine serum were obtained from Sigma Aldrich at Saint Louis present in the United States of America. All other chemicals used were of grade use for analytical purposes [16].…”

Section: Chemicalsmentioning

confidence: 99%

“…On 17-10 days of study, all the mice except in the control category were administered with CYP (25 mg/kg, intraperitoneal) 1 h after administration of the extract or vehicle. Blood samples were collected on day 20 and total count of cells in blood was estimated utilizing a hematology analyzer from Arcus, Diatron, Wien, Austria [16,19,20].…”

Section: Cyp-induced Myelosuppressionmentioning

confidence: 99%

Evaluation of in Vivo Immunomodulatory Activity of Aqueous and Ethanolic Extract of Capparis Moonii

Kanase

SHETTIGAR

2020

Asian J Pharm Clin Res

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Objective: The present study was intended to evaluate the in vivo immunomodulatory activity of aqueous and ethanolic extract of dried fruits of Capparis moonii Wight. Methods: The effect of both the extracts was evaluated by carbon clearance assay in mice, and they showed a significant increase in the phagocytic index at 50, 100, and 200 mg/kg dose. The effect of the extracts on delayed-type hypersensitivity (DTH) and antibody titer assay was evaluated in ovalbumin immunized mice. Results: Both extracts showed a significant effect on DTH response and stimulation of antibody titer at 200 mg/kg dose. The effects of the extracts in cyclophosphamide-induced myelosuppression mice were also statistically significant. Conclusion: The studies demonstrated specific and non-specific immunostimulating properties of both the ethanol and aqueous extract of C. moonii fruits in various in vivo experimental methods suggesting its therapeutic usefulness in immunocompromised conditions.

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A Review on Eulophia nuda Immunomodulatory activity in Peritoneal macrophages and an assessment of its in-vivo activity in mice

R. Thakre,

Paithnakar,

Vyas

et al. 2023

AJPR

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The purpose of this review is to highlight the findings of research on immunomodulatory activity of eulophia nuda tubers. Taxonomically, the genus Eulophia and the genus Dioscorea are represented in Amarkand. Pharmacologically, this plant has cytotoxic, antioxidant, antiglycation, and DNA protective activities. The selection of papers was made using the most relevant databases for the pharmacological activity on the basis of their phytochemical constituents. The review also goes over different in-vitro assay techniques and animal study conformation. The effect of Eulophia nuda extracts on the release of mediators such as nitric oxide (NO), superoxide, lysosomal enzyme, and myeloperoxidase activity of isolated murine peritoneal macrophages was studied at different doses. Eulophia nuda extracts were tested biochemically, hematologically, serologically, and histopathologically. The effect of eulophia nuda extracts on delayed-type hypersensitivity (DTH), serum antibody response, and cyclophosphamide-induced myelosuppression in Swiss albino mice was studied. The current work reviews the histopathological, biological, and statistical analysis of eulophia nuda extract on animal models.

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Evaluation of in Vivo Immunomodulatory Activity of Aqueous and Ethanolic Extract of Eulophia Nuda L.

Cited by 3 publications

References 15 publications

Modulatory Effects of Aqueous Leaf Extract of Gongronema latifolium (asclepiadaceae) on Some Natural and Adaptive Immune Responses in Rodents

Modulatory Effects of Aqueous Leaf Extract of Gongronema latifolium (asclepiadaceae) on Some Natural and Adaptive Immune Responses in Rodents

Evaluation of in Vivo Immunomodulatory Activity of Aqueous and Ethanolic Extract of Capparis Moonii

A Review on Eulophia nuda Immunomodulatory activity in Peritoneal macrophages and an assessment of its in-vivo activity in mice

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