Evaluation of in Vitro Immunomodulatory Activity of Aqueous and Ethanolic Extract of Eulophia Nuda Lindl

Abstract

Objective: The aim of this study was to evaluate the in vitro immunomodulatory activity of aqueous and ethanolic extract of dried tubers of Eulophia nuda.Methods: Effect of both the extracts was evaluated at various concentrations (832–6.5 μg/ml) for secretion of mediators such as nitric oxide (NO), superoxide, lysosomal enzyme, and myeloperoxidase activity of isolated murine peritoneal macrophages.Results: The extracts showed stimulation of NO, statistically significant at 832 μg/ml (SI 1.739) for ENA and at 832 μg/ml (stimulation index [SI] 1.662) for ENE; significant stimulation on lysosomal enzyme release for ENA at 832 μg/ml (SI 1.404) and ENE at 832 μg/ml (SI 1.513); myeloperoxidase activity was statistically significant for ENA at 832 μg/ml (SI 1.728) and ENE at 832 μg/ml (SI 1.770).Conclusion: In vitro phagocytic index showed significant results and thus proving the need for confirmation through in vivo studies.

Evaluation of in Vivo Immunomodulatory Activity of Aqueous and Ethanolic Extract of Eulophia Nuda L.

“…Higher reduction of nitroblue tetrazolium (NBT) dye by ENA and ENE extract represents a higher activity of oxidase enzyme, reflecting stimulation of phagocytes in proportion to intracellular killing. For lysosomal enzyme activity, the transformation of para-nitrophenylphosphate to colored compound by the acid phosphatase of the stimulated macrophages correlates to the extent of degranulation in phagocytosis [6,29]. Phagocytosis of particles by macrophages is usually accompanied by a burst of oxidative metabolism allowing the generation of reactive oxygen species which can be detected through an assay based on the reduction of NBT [30].…”

“…Development of agents capable of moving "patients" immune system from a state of immune deficiency to one or more normal function would likely to have a significant impact on disease and the patient it affects. Such agent would not be a cure but would control the manifestation and course of disease [5][6][7].…”